We are hiring!

Strain identifier

BacDive ID: 717

Type strain: No

Species: Priestia megaterium

Strain history: <- IMG 1521

NCBI tax ID(s): 592022 (strain), 1404 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 7.1 (current version):
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 7.1 (current version)

General

@ref: 105

BacDive-ID: 717

DSM-Number: 319

keywords: genome sequence, Bacteria, mesophilic, human pathogen

description: Priestia megaterium DSM 319 is a mesophilic human pathogen of the family Bacillaceae.

NCBI tax id

NCBI tax idMatching level
592022strain
1404species

strain history: <- IMG 1521

doi: 10.13145/bacdive717.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Priestia
  • species: Priestia megaterium
  • full scientific name: Priestia megaterium (de Bary 1884) Gupta et al. 2020
  • synonyms

    • @ref: 20215
    • synonym: Bacillus megaterium

@ref: 105

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Priestia

species: Priestia megaterium

full scientific name: Priestia megaterium (de Bary 1884) Gupta et al. 2020

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes86.71
6948093.396positive

Culture and growth conditions

culture medium

  • @ref: 105
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 105
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 95.83

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 70.72

Safety information

risk assessment

  • @ref: 105
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Priestia megaterium DSM 319 DSM 319GCA_000025805completencbi592022
66792Bacillus megaterium DSM 319592022.4completepatric592022
66792Bacillus megaterium DSM 319646564507completeimg592022
66792Cobetia amphilecti B2M13GCA_018860945scaffoldpatric1055104

GC content

  • @ref: 105
  • GC-content: 38.1

External links

@ref: 105

culture collection no.: DSM 319

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/156585

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1452037Cloning and sequencing of the genes encoding glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase (gap operon) from mesophilic Bacillus megaterium: comparison with corresponding sequences from thermophilic Bacillus stearothermophilus.Schlapfer BS, Zuber HGene10.1016/0378-1119(92)90031-j1992Amino Acid Sequence, Bacillus megaterium/*enzymology/genetics, Base Sequence, Cloning, Molecular, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Exons, Geobacillus stearothermophilus/*enzymology/genetics, Glyceraldehyde-3-Phosphate Dehydrogenases/*genetics/metabolism, Hot Temperature, Introns, Molecular Sequence Data, Open Reading Frames, *Operon, Phosphoglycerate Kinase/*genetics/metabolism, Restriction Mapping, Triose-Phosphate Isomerase/*genetics/metabolismMetabolism
Enzymology7764969Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319.Meinhardt F, Busskamp M, Wittchen KDAppl Microbiol Biotechnol10.1007/BF0022123019943-Isopropylmalate Dehydrogenase, Alcohol Oxidoreductases/chemistry/*genetics, Amino Acid Sequence, Bacillus megaterium/*genetics/physiology, Bacterial Proteins/chemistry/*genetics, Base Sequence, Cloning, Molecular, DNA, Bacterial, Endopeptidases/chemistry/*genetics, *Genes, Bacterial, *Membrane Proteins, Molecular Sequence Data, Mutagenesis, InsertionalGenetics
Enzymology7766087Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement.Wittchen KD, Meinhardt FAppl Microbiol Biotechnol10.1007/BF001911841995Bacillus megaterium/*enzymology/*genetics, *Bacterial Proteins, Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA, Bacterial/genetics, Endopeptidases/*genetics/metabolism, Escherichia coli/genetics, Gene Deletion, Genes, Bacterial, Genetic Techniques, Genetic Vectors, Molecular Sequence Data, Mutation, Plasmids/geneticsGenetics
Enzymology9210344Plasmids for efficient single-copy gene cloning into gdh2 and trpC of Bacillus megaterium DSM319 and QM B1551.Schmiedel D, Vary PS, Jablonski L, Hillen WAppl Microbiol Biotechnol10.1007/s0025300509701997Bacillus megaterium/*genetics, Chromosomes, Bacterial, Cloning, Molecular, *Plasmids
Metabolism10322036Regulation of beta-galactosidase expression in Bacillus megaterium DSM319 by a XylS/AraC-type transcriptional activator.Strey J, Wittchen KD, Meinhardt FJ Bacteriol10.1128/JB.181.10.3288-3292.19991999Bacillus megaterium/enzymology/*genetics, Bacterial Proteins, Base Sequence, Binding Sites, Cloning, Molecular, DNA-Binding Proteins, Gene Deletion, Gene Expression, *Gene Expression Regulation, Bacterial/drug effects, Gene Expression Regulation, Enzymologic/drug effects, Genes, Bacterial/*genetics/physiology, Glucose/metabolism/pharmacology, Lactose/metabolism/pharmacology, Models, Genetic, Molecular Sequence Data, Mutagenesis, Insertional, Nitrophenylgalactosides/metabolism, Open Reading Frames/genetics, Operon/genetics, Promoter Regions, Genetic/genetics, Sequence Analysis, DNA, Trans-Activators/genetics/*metabolism, Transcription, Genetic/drug effects, beta-Galactosidase/*genetics/metabolismGenetics
Enzymology11499932Cloning, expression, and carbon catabolite repression of the bamM gene encoding beta-amylase of Bacillus megaterium DSM319.Lee JS, Wittchen KD, Stahl C, Strey J, Meinhardt FAppl Microbiol Biotechnol10.1007/s0025301006452001Amino Acid Sequence, Bacillus megaterium/*enzymology/genetics, Base Sequence, Carbon/*metabolism, Cloning, Molecular, *Enzyme Repression, Molecular Sequence Data, Mutagenesis, Site-Directed, *Response Elements, beta-Amylase/*geneticsGenetics
12501411Molecular characterization of the operon comprising the spoIV gene of Bacillus megaterium DSM319 and generation of a deletion mutant.Wittchen KD, Strey J, Bultmann AJ Gen Appl Microbiol10.2323/jgam.44.3171998
Metabolism14593507Identification and functional characterization of a type I signal peptidase gene of Bacillus megaterium DSM319.Nahrstedt H, Wittchen K, Rachman MA, Meinhardt FAppl Microbiol Biotechnol10.1007/s00253-003-1469-22003Amino Acid Sequence, Bacillus megaterium/*enzymology/*genetics, Bacterial Proteins/genetics, Base Sequence, Cellulase/genetics/metabolism, DNA, Bacterial/chemistry/isolation & purification, Gene Deletion, Gene Dosage, Gene Expression, Gene Order, Genes, Bacterial, Genes, Essential, Genes, Reporter, Membrane Proteins/*genetics/*metabolism, Molecular Sequence Data, Mutagenesis, Insertional, Operon, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Serine Endopeptidases/*genetics/*metabolism, Transcription, GeneticEnzymology
Genetics15758224Evidence for two recA genes mediating DNA repair in Bacillus megaterium.Nahrstedt H, Schroder C, Meinhardt FMicrobiology (Reading)10.1099/mic.0.27626-02005Bacillus megaterium/*enzymology/genetics/radiation effects, Bacterial Proteins/biosynthesis/*genetics, Base Sequence, DNA Damage, *DNA Repair, *Gene Expression Regulation, Bacterial, Molecular Sequence Data, Rec A Recombinases/biosynthesis/*genetics, *SOS Response, Genetics, Ultraviolet RaysEnzymology
Genetics16567015A protein database constructed from low-coverage genomic sequence of Bacillus megaterium and its use for accelerated proteomic analysis.Sun J, Wang W, Hundertmark C, Zeng AP, Jahn D, Deckwer WDJ Biotechnol10.1016/j.jbiotec.2006.01.0332006Algorithms, Bacillus megaterium/*genetics, Bacterial Proteins/*genetics, Base Sequence, Chromosome Mapping/*methods, *Databases, Protein, Genome, Bacterial/genetics, Information Storage and Retrieval/methods, Molecular Sequence Data, Peptide Mapping/*methods, Proteome/*genetics, Proteomics/methods, Sequence Alignment/methods, Sequence Analysis, DNA/*methodsTranscriptome
Biotechnology17022804Comparative proteomic analysis of high cell density cultivations with two recombinant Bacillus megaterium strains for the production of a heterologous dextransucrase.Wang W, Hollmann R, Deckwer WDProteome Sci10.1186/1477-5956-4-192006Cultivation
Phylogeny17086410Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates.Furch T, Hollmann R, Wittmann C, Wang W, Deckwer WDBioprocess Biosyst Eng10.1007/s00449-006-0095-72006Bacillus megaterium/classification/growth & development/*metabolism, Carbon Isotopes, Citric Acid Cycle, Kinetics, Models, Theoretical, Pentose Phosphate Pathway, Species Specificity, Substrate SpecificityEnzymology
Enzymology17608626Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium.Homann A, Biedendieck R, Gotze S, Jahn D, Seibel JBiochem J10.1042/BJ200706002007Amino Acids, Bacillus megaterium/*enzymology, Catalytic Domain, Cloning, Molecular, Conserved Sequence, Escherichia coli/genetics, Hexosyltransferases/*genetics/*metabolism, Kinetics, Mutagenesis, Site-Directed, Oligosaccharides/biosynthesis, Polysaccharides/biosynthesisMetabolism
Biotechnology17657486Bacillus megaterium--from simple soil bacterium to industrial protein production host.Vary PS, Biedendieck R, Fuerch T, Meinhardt F, Rohde M, Deckwer WD, Jahn DAppl Microbiol Biotechnol10.1007/s00253-007-1089-32007*Bacillus megaterium/enzymology/genetics/metabolism, Genetic Engineering/*methods, *Industrial Microbiology, Plasmids/genetics, Recombinant Proteins/genetics/*metabolism, *Soil MicrobiologyMetabolism
Enzymology19149186[Cloning, expression and the characterization of beta-amylase from a Bacillus megaterium WS06].Wu J, Zhang SSheng Wu Gong Cheng Xue Bao2008Bacillus megaterium/*enzymology/genetics, Cloning, Molecular, Escherichia coli/genetics/*metabolism, Genetic Vectors/genetics, Recombinant Proteins/biosynthesis/genetics/*metabolism, Sequence Analysis, Protein, Sequence Homology, Amino Acid, Temperature, beta-Amylase/biosynthesis/*genetics/metabolismMetabolism
Metabolism21538108Functional analysis of the response regulator DegU in Bacillus megaterium DSM319 and comparative secretome analysis of degSU mutants.Borgmeier C, Voigt B, Hecker M, Meinhardt FAppl Microbiol Biotechnol10.1007/s00253-011-3302-72011Amino Acid Sequence, Amylases/genetics/metabolism, Bacillus megaterium/*genetics/*metabolism, Bacillus subtilis/genetics/metabolism, Bacterial Proteins/genetics/*metabolism, Extracellular Matrix Proteins, Gene Knockout Techniques, Hexosyltransferases/biosynthesis/genetics, Mass Spectrometry, Mutation, Peptide Hydrolases/genetics/metabolism, Polymerase Chain Reaction, Protein Sorting Signals/genetics, Proteome, Sequence Alignment, Sequence Deletion/geneticsProteome
Genetics21705586Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319.Eppinger M, Bunk B, Johns MA, Edirisinghe JN, Kutumbaka KK, Koenig SS, Creasy HH, Rosovitz MJ, Riley DR, Daugherty S, Martin M, Elbourne LD, Paulsen I, Biedendieck R, Braun C, Grayburn S, Dhingra S, Lukyanchuk V, Ball B, Ul-Qamar R, Seibel J, Bremer E, Jahn D, Ravel J, Vary PSJ Bacteriol10.1128/JB.00449-112011Bacillus megaterium/*classification/*genetics/metabolism, Chromosomes, Bacterial, Flagella/genetics/metabolism, Gene Expression Regulation, Bacterial/physiology, Genetic Variation, *Genome, Bacterial, Molecular Sequence Data, Phylogeny, Plasmids, Species SpecificityPhylogeny
Enzymology23155054Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway.Moore SJ, Biedendieck R, Lawrence AD, Deery E, Howard MJ, Rigby SE, Warren MJJ Biol Chem10.1074/jbc.M112.4225352012Bacillus megaterium/*metabolism, Biosynthetic Pathways, Catalysis, Cloning, Molecular, Cobalt/chemistry, Cobamides/chemistry, Dithiothreitol/chemistry, Electron Spin Resonance Spectroscopy, *Gene Expression Regulation, Enzymologic, Iron-Sulfur Proteins, Magnetic Resonance Spectroscopy/methods, Mass Spectrometry/methods, Models, Chemical, Models, Molecular, Molecular Conformation, Mutagenesis, Mutagenesis, Site-Directed, Oxygen/chemistry, Recombinant Proteins/chemistry, Solubility, Vitamin B 12/*biosynthesis/*chemistryMetabolism
Metabolism23797329A new cytochrome P450 system from Bacillus megaterium DSM319 for the hydroxylation of 11-keto-beta-boswellic acid (KBA).Brill E, Hannemann F, Zapp J, Bruning G, Jauch J, Bernhardt RAppl Microbiol Biotechnol10.1007/s00253-013-5029-02013Bacillus megaterium/*enzymology, Cytochrome P-450 Enzyme System/*metabolism, Hydroxylation, Triterpenes/*metabolismEnzymology
Enzymology23996139Extracellular L-asparaginase from a protease-deficient Bacillus aryabhattai ITBHU02: purification, biochemical characterization, and evaluation of antineoplastic activity in vitro.Singh Y, Gundampati RK, Jagannadham MV, Srivastava SKAppl Biochem Biotechnol10.1007/s12010-013-0455-02013Antineoplastic Agents/chemistry/isolation & purification/metabolism/pharmacology, Asparaginase/biosynthesis/chemistry/*isolation & purification/*pharmacology, Bacillus/*cytology/enzymology/*metabolism, Cell Line, Tumor, Enzyme Stability, Extracellular Space/*enzymology, Humans, Hydrogen-Ion Concentration, Kinetics, Peptide Hydrolases/*deficiency, Protein Structure, Secondary, Substrate Specificity, TemperaturePhylogeny
Metabolism24657453Towards a cell factory for vitamin B12 production in Bacillus megaterium: bypassing of the cobalamin riboswitch control elements.Moore SJ, Mayer MJ, Biedendieck R, Deery E, Warren MJN Biotechnol10.1016/j.nbt.2014.03.0032014Bacillus megaterium/*metabolism, Cobalt/metabolism, Vitamin B 12/*biosynthesisBiotechnology
Metabolism25904128Comparison of CYP106A1 and CYP106A2 from Bacillus megaterium - identification of a novel 11-oxidase activity.Kiss FM, Schmitz D, Zapp J, Dier TK, Volmer DA, Bernhardt RAppl Microbiol Biotechnol10.1007/s00253-015-6563-8201517-Hydroxycorticosteroids/*metabolism, Bacillus megaterium/*enzymology, Cytochrome P-450 Enzyme System/*metabolism, Magnetic Resonance Spectroscopy, Substrate SpecificityEnzymology
Enzymology26188546Identification of new substrates for the CYP106A1-mediated 11-oxidation and investigation of the reaction mechanism.Kiss FM, Khatri Y, Zapp J, Bernhardt RFEBS Lett10.1016/j.febslet.2015.07.0112015Androstenedione/*analogs & derivatives/chemistry/metabolism, Bacillus megaterium/enzymology, Cytochrome P-450 Enzyme System/*metabolism, Deuterium/chemistry, Kinetics, Oxidation-Reduction, Protein Binding, Solvents/chemistry, Substrate SpecificityMetabolism
Metabolism27686671Structural basis of steroid binding and oxidation by the cytochrome P450 CYP109E1 from Bacillus megaterium.Jozwik IK, Kiss FM, Gricman L, Abdulmughni A, Brill E, Zapp J, Pleiss J, Bernhardt R, Thunnissen AWFEBS J10.1111/febs.139112016Amino Acid Sequence, Bacillus megaterium/*enzymology/genetics, Bacterial Proteins/*chemistry/genetics/metabolism, Binding Sites/genetics, Catalytic Domain, Corticosterone/chemistry/metabolism, Crystallography, X-Ray, Cytochrome P-450 Enzyme System/*chemistry/classification/metabolism, Heme/chemistry/metabolism, Molecular Dynamics Simulation, Molecular Structure, Oxidation-Reduction, Protein Binding, Protein Domains, Sequence Homology, Amino Acid, Steroids/*chemistry/metabolism, Substrate Specificity, Testosterone/chemistry/metabolismEnzymology
Enzymology28940959Biochemical and structural characterization of CYP109A2, a vitamin D3 25-hydroxylase from Bacillus megaterium.Abdulmughni A, Jozwik IK, Brill E, Hannemann F, Thunnissen AWH, Bernhardt RFEBS J10.1111/febs.142762017Amino Acid Sequence, Bacillus megaterium/*enzymology, Binding Sites, Biocatalysis, Catalytic Domain, Cholestanetriol 26-Monooxygenase/*chemistry/*metabolism, Crystallography, X-Ray, Hydroxylation, Kinetics, Oxidation-Reduction, Phylogeny, Protein Conformation, Sequence Homology, Substrate SpecificityPhylogeny
Metabolism30398298CYP109E1 from Bacillus megaterium Acts as a 24- and 25-Hydroxylase for Cholesterol.Putkaradze N, Litzenburger M, Hutter MC, Bernhardt RChembiochem10.1002/cbic.2018005952019Adrenodoxin/metabolism, Bacillus megaterium/*metabolism, Biotransformation, Cholesterol/analogs & derivatives/metabolism, Cytochrome P-450 Enzyme System/*physiology, Escherichia coli/*metabolism, Ferredoxin-NADP Reductase/metabolism, Hydroxylation, Mixed Function Oxygenases/*physiology, Oxidation-Reduction, Substrate SpecificityEnzymology
30704007First Report of a Leaf Spot of Radermachera sinica in China Caused by Bacillus megaterium.Li YL, Zhou Z, Yuan YC, Ye JRPlant Dis10.1094/PDIS-05-14-0469-PDN2014
Genetics31822710Manually curated genome-scale reconstruction of the metabolic network of Bacillus megaterium DSM319.Aminian-Dehkordi J, Mousavi SM, Jafari A, Mijakovic I, Marashi SASci Rep10.1038/s41598-019-55041-w2019Bacillus megaterium/genetics/*metabolism, Fatty Acids/biosynthesis, Feasibility Studies, *Genome, Bacterial, Genomics, Industrial Microbiology, Lipid Metabolism/genetics, Metabolic Engineering, Metabolic Networks and Pathways/*genetics, Metabolomics, *Models, BiologicalMetabolism
Phylogeny31863264Phage vB_BmeM-Goe8 infecting Bacillus megaterium DSM319.Baena Lozada LP, Hoppert M, Hertel RArch Virol10.1007/s00705-019-04513-52019Bacillus Phages/*classification/genetics/*isolation & purification/ultrastructure, Bacillus megaterium/*virology, Base Composition, Cluster Analysis, *Genes, Viral, *Genome, Viral, Myoviridae/*classification/genetics/*isolation & purification/ultrastructure, Phylogeny, Sequence Analysis, DNA, Sequence Homology, Terminal Repeat Sequences, Virion/ultrastructureEnzymology
Biotechnology32582661A Systems-Based Approach for Cyanide Overproduction by Bacillus megaterium for Gold Bioleaching Enhancement.Aminian-Dehkordi J, Mousavi SM, Marashi SA, Jafari A, Mijakovic IFront Bioeng Biotechnol10.3389/fbioe.2020.005282020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
105Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 319)https://www.dsmz.de/collection/catalogue/details/culture/DSM-319
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)