Strain identifier

BacDive ID: 7166

Type strain: Yes

Species: Methylobacterium fujisawaense

Strain Designation: O-31

Strain history: CIP <- 1993, NCIMB <- K. Kouno, Fujisawa, Japan: strain O-31

NCBI tax ID(s): 107400 (species)

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General

@ref: 2283

BacDive-ID: 7166

DSM-Number: 5686

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Methylobacterium fujisawaense O-31 is an aerobe, mesophilic, motile bacterium of the family Methylobacteriaceae.

NCBI tax id

  • NCBI tax id: 107400
  • Matching level: species

strain history

@refhistory
2283<- NCIMB <- K. Kouno
67770NCIMB 12417 <-- K. Kouno 0-31.
119480CIP <- 1993, NCIMB <- K. Kouno, Fujisawa, Japan: strain O-31

doi: 10.13145/bacdive7166.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylobacteriaceae
  • genus: Methylobacterium
  • species: Methylobacterium fujisawaense
  • full scientific name: Methylobacterium fujisawaense Green et al. 1988

@ref: 2283

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylobacteriaceae

genus: Methylobacterium

species: Methylobacterium fujisawaense

full scientific name: Methylobacterium fujisawaense Green et al. 1988

strain designation: O-31

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes93.764
69480100negative
119480yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2283COLBY AND ZATHMAN MEDIUM (DSMZ Medium 606)yeshttps://mediadive.dsmz.de/medium/606Name: COLBY AND ZATHMAN MEDIUM (DSMZ Medium 606) Composition: Agar 15.0 g/l Methanol 1.584 g/l K2HPO4 1.2 g/l KH2PO4 0.62 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l NaCl 0.1 g/l CaCl2 x 6 H2O 0.05 g/l FeCl3 x 6 H2O 0.001 g/l ZnSO4 x 7 H2O 7e-05 g/l Na2MoO4 x 2 H2O 1e-05 g/l H3BO3 1e-05 g/l MnSO4 x 5 H2O 1e-05 g/l CuSO4 x 5 H2O 5e-06 g/l CoCl2 x 6 H2O 5e-06 g/l Distilled water
40785MEDIUM 53 - for Marinomonas and Methylobacterium (except Methylobacterium organophilum)yesDistilled water make up to (1000.000 ml);Methanol (5.000 ml);Brain heartinfusion (37.000 g)
119480CIP Medium 53yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=53

culture temp

@refgrowthtypetemperaturerange
2283positivegrowth30mesophilic
40785positivegrowth30mesophilic
53488positivegrowth30mesophilic
67770positivegrowth30mesophilic
119480positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 53488
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.997

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11948016947citrate-carbon source
11948017632nitrate-reduction
11948016301nitrite-reduction

metabolite production

  • @ref: 119480
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 119480
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
119480oxidase+
119480beta-galactosidase-3.2.1.23
119480alcohol dehydrogenase-1.1.1.1
119480amylase-
119480DNase-
119480caseinase-3.4.21.50
119480catalase+1.11.1.6
119480tween esterase-
119480lecithinase-
119480lysine decarboxylase-4.1.1.18
119480ornithine decarboxylase-4.1.1.17
119480urease+3.5.1.5

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119480+-+-----------------++------+--+-------+---++----+---+------+--------------+---++-+-----+-+-------+

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsampling date
40785JapanJPNAsia
53488JapanJPNAsia1975
119480JapanJPNAsia

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
22831Risk group (German classification)
1194801Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Methylobacterium fujisawaense gene for 16S ribosomal RNA, partial sequence, strain:DSM 5686AB1756341433ena107400
20218Methylobacterium fujisawaense 16S rRNA gene, strain DSM 5686AJ2508011440ena107400
20218Methylobacterium sp. OS-16.b partial 16S rRNA gene, isolate OS-16.bAM2373441435ena378774

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methylobacterium fujisawaense DSM 5686GCA_014138435scaffoldncbi107400
66792Methylobacterium fujisawaense strain DSM 5686107400.3wgspatric107400
66792Methylobacterium fujisawaense DSM 56862829760844draftimg107400

GC content

  • @ref: 67770
  • GC-content: 71.8
  • method: Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes88.549no
flagellatedno71.717no
gram-positiveno97.586no
anaerobicno98.45yes
aerobicyes88.562no
halophileno90.985no
spore-formingno93.719no
glucose-utilyes87.641no
thermophileno99.378yes
glucose-fermentno88.165no

External links

@ref: 2283

culture collection no.: DSM 5686, ATCC 43884, NCIMB 12417, CCUG 36914, CIP 103775, IFO 15843, JCM 10890, BCRC 17093, NBRC 15843

straininfo link

  • @ref: 76619
  • straininfo: 42104

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18175690Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea.Weon HY, Kim BY, Joa JH, Son JA, Song MH, Kwon SW, Go SJ, Yoon SHInt J Syst Evol Microbiol10.1099/ijs.0.65047-02008*Air Microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Korea, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny20495043Methylobacterium marchantiae sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the thallus of a liverwort.Schauer S, Kampfer P, Wellner S, Sproer C, Kutschera UInt J Syst Evol Microbiol10.1099/ijs.0.021915-02010Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fimbriae, Bacterial/physiology, Hepatophyta/*microbiology, Locomotion, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pigments, Biological/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2283Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5686)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5686
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40785Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15609
53488Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 36914)https://www.ccug.se/strain?id=36914
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76619Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42104.1StrainInfo: A central database for resolving microbial strain identifiers
119480Curators of the CIPCollection of Institut Pasteur (CIP 103775)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103775