Strain identifier

BacDive ID: 7137

Type strain: Yes

Species: Methanothermus fervidus

Strain Designation: V24 S

Strain history: DSM 2088 <-- K. O. Stetter V24S.

NCBI tax ID(s): 523846 (strain), 2180 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 717

BacDive-ID: 7137

DSM-Number: 2088

keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic, Gram-negative

description: Methanothermus fervidus V24 S is an anaerobe, hyperthermophilic, Gram-negative archaeon that was isolated from hot solfataric spring.

NCBI tax id

NCBI tax idMatching level
523846strain
2180species

strain history

@refhistory
717<- K. O. Stetter, Univ. Regensburg, Germany; V24 S <- K. O. Stetter {1980}
67770DSM 2088 <-- K. O. Stetter V24S.

doi: 10.13145/bacdive7137.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanothermaceae
  • genus: Methanothermus
  • species: Methanothermus fervidus
  • full scientific name: Methanothermus fervidus Stetter 1982

@ref: 717

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanothermaceae

genus: Methanothermus

species: Methanothermus fervidus

full scientific name: Methanothermus fervidus Stetter 1982

strain designation: V24 S

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.986

multimedia

  • @ref: 66793
  • multimedia content: EM_DSM_2088_1.jpg
  • caption: electron microscopic image
  • intellectual property rights: © HZI/Manfred Rohde

Culture and growth conditions

culture medium

  • @ref: 717
  • name: METHANOTHERMUS MEDIUM (DSMZ Medium 203)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/203
  • composition: Name: METHANOTHERMUS MEDIUM (DSMZ Medium 203) Composition: Na2SO4 3.36967 g/l Trypticase peptone 1.98216 g/l Yeast extract 1.98216 g/l Na2CO3 0.99108 g/l L-Cysteine HCl x H2O 0.49554 g/l Na2S x 9 H2O 0.49554 g/l K2HPO4 0.222993 g/l (NH4)2SO4 0.222993 g/l KH2PO4 0.222993 g/l MgSO4 x 7 H2O 0.0297324 g/l Nitrilotriacetic acid 0.0148662 g/l NaCl 0.0099108 g/l MnSO4 x H2O 0.0049554 g/l CoSO4 x 7 H2O 0.00178394 g/l ZnSO4 x 7 H2O 0.00178394 g/l FeSO4 x 7 H2O 0.00099108 g/l CaCl2 x 2 H2O 0.00099108 g/l Sodium resazurin 0.00049554 g/l NiCl2 x 6 H2O 0.000297324 g/l AlK(SO4)2 x 12 H2O 0.000198216 g/l CuSO4 x 5 H2O 9.9108e-05 g/l H3BO3 9.9108e-05 g/l Na2MoO4 x 2 H2O 9.9108e-05 g/l Pyridoxine hydrochloride 9.9108e-05 g/l p-Aminobenzoic acid 4.9554e-05 g/l Calcium D-(+)-pantothenate 4.9554e-05 g/l Nicotinic acid 4.9554e-05 g/l Riboflavin 4.9554e-05 g/l Thiamine HCl 4.9554e-05 g/l (DL)-alpha-Lipoic acid 4.9554e-05 g/l Folic acid 1.98216e-05 g/l Biotin 1.98216e-05 g/l Na2WO4 x 2 H2O 3.96432e-06 g/l Na2SeO3 x 5 H2O 2.97324e-06 g/l Vitamin B12 9.9108e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
717positivegrowth83hyperthermophilic
67770positivegrowth80hyperthermophilic
69480thermophilic99.999

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
717anaerobe
69480anaerobe99.604

spore formation

@refspore formationconfidence
69481no95
69480no99.095

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
717hot solfataric springIcelandISLEurope
67770Hot springIcelandISLEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Sulfuric
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 717
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Blautia coccoides strain DSM 2088 16S ribosomal RNA gene, partial sequenceM590901478ena1532
717Methanothermus fervidus 16S ribosomal RNAM591451479ena2180

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanothermus fervidus DSM 2088GCA_000166095completencbi523846
66792Methanothermus fervidus V24S, DSM 2088649633067completeimg523846
66792Methanothermus fervidus DSM 2088523846.9completepatric523846

GC content

@refGC-contentmethod
71733.0
6777033thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno95no
motileno86.67no
flagellatedno95.864no
gram-positiveno96no
anaerobicyes99.088yes
aerobicno96.555yes
halophileno90.194no
spore-formingno95.554no
thermophileyes99.445no
glucose-utilyes70.411no
glucose-fermentno57.812no

External links

@ref: 717

culture collection no.: DSM 2088, ATCC 43054, JCM 10308

straininfo link

  • @ref: 76591
  • straininfo: 47544

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
717Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2088)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2088
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76591Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47544.1StrainInfo: A central database for resolving microbial strain identifiers