Strain identifier
BacDive ID: 7137
Type strain: 
Species: Methanothermus fervidus
Strain Designation: V24 S
Strain history: DSM 2088 <-- K. O. Stetter V24S.
NCBI tax ID(s): 523846 (strain), 2180 (species)
version 9.3 (current version)
General
@ref: 717
BacDive-ID: 7137
DSM-Number: 2088
keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Methanothermus fervidus V24 S is an anaerobe, hyperthermophilic archaeon that was isolated from hot solfataric spring.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 523846 | strain |
| 2180 | species |
strain history
| @ref | history |
|---|---|
| 717 | <- K. O. Stetter, Univ. Regensburg, Germany; V24 S <- K. O. Stetter {1980} |
| 67770 | DSM 2088 <-- K. O. Stetter V24S. |
doi: 10.13145/bacdive7137.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanothermaceae
- genus: Methanothermus
- species: Methanothermus fervidus
- full scientific name: Methanothermus fervidus Stetter 1982
@ref: 717
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanothermaceae
genus: Methanothermus
species: Methanothermus fervidus
full scientific name: Methanothermus fervidus Stetter 1982
strain designation: V24 S
type strain: yes
Morphology
multimedia
- @ref: 66793
- multimedia content: EM_DSM_2088_1.jpg
- caption: electron microscopic image
- intellectual property rights: © HZI/Manfred Rohde
Culture and growth conditions
culture medium
- @ref: 717
- name: METHANOTHERMUS MEDIUM (DSMZ Medium 203)
- growth: yes
- link: https://mediadive.dsmz.de/medium/203
- composition: Name: METHANOTHERMUS MEDIUM (DSMZ Medium 203) Composition: Na2SO4 3.36967 g/l Trypticase peptone 1.98216 g/l Yeast extract 1.98216 g/l Na2CO3 0.99108 g/l L-Cysteine HCl x H2O 0.49554 g/l Na2S x 9 H2O 0.49554 g/l K2HPO4 0.222993 g/l (NH4)2SO4 0.222993 g/l KH2PO4 0.222993 g/l MgSO4 x 7 H2O 0.0297324 g/l Nitrilotriacetic acid 0.0148662 g/l NaCl 0.0099108 g/l MnSO4 x H2O 0.0049554 g/l CoSO4 x 7 H2O 0.00178394 g/l ZnSO4 x 7 H2O 0.00178394 g/l FeSO4 x 7 H2O 0.00099108 g/l CaCl2 x 2 H2O 0.00099108 g/l Sodium resazurin 0.00049554 g/l NiCl2 x 6 H2O 0.000297324 g/l AlK(SO4)2 x 12 H2O 0.000198216 g/l CuSO4 x 5 H2O 9.9108e-05 g/l H3BO3 9.9108e-05 g/l Na2MoO4 x 2 H2O 9.9108e-05 g/l Pyridoxine hydrochloride 9.9108e-05 g/l p-Aminobenzoic acid 4.9554e-05 g/l Calcium D-(+)-pantothenate 4.9554e-05 g/l Nicotinic acid 4.9554e-05 g/l Riboflavin 4.9554e-05 g/l Thiamine HCl 4.9554e-05 g/l (DL)-alpha-Lipoic acid 4.9554e-05 g/l Folic acid 1.98216e-05 g/l Biotin 1.98216e-05 g/l Na2WO4 x 2 H2O 3.96432e-06 g/l Na2SeO3 x 5 H2O 2.97324e-06 g/l Vitamin B12 9.9108e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 717 | positive | growth | 83 |
| 67770 | positive | growth | 80 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 717 | anaerobe | |
| 125438 | anaerobe | 90.127 |
| 125439 | anaerobe | 97 |
spore formation
- @ref: 125438
- spore formation: no
- confidence: 91.342
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 717 | hot solfataric spring | Iceland | ISL | Europe |
| 67770 | Hot spring | Iceland | ISL | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Sulfuric | |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 717
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 717
- description: Methanothermus fervidus 16S ribosomal RNA
- accession: M59145
- length: 1479
- database: nuccore
- NCBI tax ID: 2180
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanothermus fervidus DSM 2088 | GCA_000166095 | complete | ncbi | 523846 |
| 66792 | Methanothermus fervidus V24S, DSM 2088 | 649633067 | complete | img | 523846 |
| 66792 | Methanothermus fervidus DSM 2088 | 523846.9 | complete | patric | 523846 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 717 | 33.0 | |
| 67770 | 33 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 51.743 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 90.127 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 89.335 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 91.342 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | yes | 70.134 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | no | 79.458 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 52.8 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 66 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | variable | 71.6 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | anaerobe | 97 |
External links
@ref: 717
culture collection no.: DSM 2088, ATCC 43054, JCM 10308
straininfo link
- @ref: 76591
- straininfo: 47544
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 717 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2088) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2088 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 76591 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47544.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |||
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |