Strain identifier

BacDive ID: 7122

Type strain: No

Species: Methanosarcina thermophila

Strain Designation: MST-A1

Strain history: <- J. P. Touzel, INSERM, Villeneuve d'Ascq, France; MST-A1 <- E. Samain {1981}

NCBI tax ID(s): 2210 (species)

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General

@ref: 1238

BacDive-ID: 7122

DSM-Number: 2905

keywords: Archaea, anaerobe, thermophilic

description: Methanosarcina thermophila MST-A1 is an anaerobe, thermophilic archaeon that was isolated from digester slugde.

NCBI tax id

  • NCBI tax id: 2210
  • Matching level: species

strain history

  • @ref: 1238
  • history: <- J. P. Touzel, INSERM, Villeneuve d'Ascq, France; MST-A1 <- E. Samain {1981}

doi: 10.13145/bacdive7122.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina thermophila
  • full scientific name: Methanosarcina thermophila Zinder et al. 1985

@ref: 1238

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina thermophila

full scientific name: Methanosarcina thermophila Zinder et al. 1985

strain designation: MST-A1

type strain: no

Culture and growth conditions

culture medium

  • @ref: 1238
  • name: METHANOSARCINA THERMOPHILA (BCYT) MEDIUM (DSMZ Medium 318)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/318
  • composition: Name: METHANOSARCINA THERMOPHILA (BCYT) MEDIUM (DSMZ Medium 318) Composition: Methanol 7.7571 g/l KHCO3 1.95886 g/l NH4Cl 0.979432 g/l Yeast extract 0.489716 g/l Trypticase peptone 0.489716 g/l Na2S x 9 H2O 0.29383 g/l KH2PO4 0.29383 g/l L-Cysteine HCl x H2O 0.29383 g/l Nitrilotriacetic acid 0.125367 g/l MgCl2 x 6 H2O 0.0979432 g/l NaCl 0.00979432 g/l FeCl2 x 4 H2O 0.00979432 g/l CaCl2 x 2 H2O 0.000979432 g/l MnCl2 x 4 H2O 0.000979432 g/l NiCl2 x 6 H2O 0.000979432 g/l ZnCl2 0.000979432 g/l Sodium resazurin 0.000489716 g/l Na2WO4 x 2 H2O 0.000391773 g/l Na2MoO4 x 2 H2O 0.00029383 g/l CoCl2 x 6 H2O 0.00029383 g/l Na2SeO3 x 5 H2O 0.00029383 g/l CuCl2 0.000195886 g/l H3BO3 9.79432e-05 g/l Pyridoxine hydrochloride 9.79432e-05 g/l Calcium D-(+)-pantothenate 4.89716e-05 g/l (DL)-alpha-Lipoic acid 4.89716e-05 g/l p-Aminobenzoic acid 4.89716e-05 g/l Nicotinic acid 4.89716e-05 g/l Riboflavin 4.89716e-05 g/l Thiamine HCl 4.89716e-05 g/l Folic acid 1.95886e-05 g/l Biotin 1.95886e-05 g/l Vitamin B12 9.79432e-07 g/l Distilled water

culture temp

  • @ref: 1238
  • growth: positive
  • type: growth
  • temperature: 55
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1238
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1238
  • sample type: digester slugde
  • geographic location: Lille
  • country: France
  • origin.country: FRA
  • continent: Europe

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Sewage sludge

Safety information

risk assessment

  • @ref: 1238
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 1238

culture collection no.: DSM 2905, OCM 96

straininfo link

  • @ref: 76577
  • straininfo: 47668

Reference

@idauthorscataloguedoi/urltitle
1238Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2905)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2905
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
76577Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47668.1StrainInfo: A central database for resolving microbial strain identifiers