Strain identifier
BacDive ID: 7119
Type strain: 
Species: Methanosarcina sp.
Strain Designation: Pr2
Strain history: <- S. N. Parshina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Pr2
NCBI tax ID(s): 2213 (species)
version 8.1 (current version)
General
@ref: 5721
BacDive-ID: 7119
DSM-Number: 15128
keywords: Archaea, anaerobe, mesophilic
description: Methanosarcina sp. Pr2 is an anaerobe, mesophilic archaeon that was isolated from pig's manure storage pond.
NCBI tax id
- NCBI tax id: 2213
- Matching level: species
strain history
- @ref: 5721
- history: <- S. N. Parshina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Pr2
doi: 10.13145/bacdive7119.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina sp.
- full scientific name: Methanosarcina Kluyver and van Niel 1936 (Approved Lists 1980)
@ref: 5721
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina sp.
full scientific name: Methanosarcina sp.
strain designation: Pr2
type strain: no
Morphology
colony morphology
- @ref: 5721
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 5721
- name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/120a
- composition: Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) Composition: Methanol 15.4688 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NaHCO3 0.830078 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
culture temp
- @ref: 5721
- growth: positive
- type: growth
- temperature: 25
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 5721
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 5721
- sample type: pig's manure storage pond
- geographic location: Crimea
- country: Russia
- origin.country: RUS
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Pond (small) |
| #Host | #Mammals | #Suidae (Pig,Swine) |
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
Sequence information
GC content
- @ref: 5721
- GC-content: 43.8
External links
@ref: 5721
culture collection no.: DSM 15128
straininfo link
- @ref: 76574
- straininfo: 404526
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5721 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15128) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15128 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 76574 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID404526.1 | StrainInfo: A central database for resolving microbial strain identifiers |