Strain identifier
BacDive ID: 7109
Type strain: 
Species: Methanosarcina sp.
Strain Designation: MTP4
Strain history: <- F. Bak, MTP4
NCBI tax ID(s): 2213 (species)
version 9.1 (current version)
General
@ref: 2641
BacDive-ID: 7109
DSM-Number: 6636
keywords: genome sequence, Archaea, anaerobe, Gram-negative
description: Methanosarcina sp. MTP4 is an anaerobe, Gram-negative archaeon that was isolated from salt marsh sediment.
NCBI tax id
- NCBI tax id: 2213
- Matching level: species
strain history
- @ref: 2641
- history: <- F. Bak, MTP4
doi: 10.13145/bacdive7109.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina sp.
- full scientific name: Methanosarcina Kluyver and van Niel 1936 (Approved Lists 1980)
@ref: 2641
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina sp.
full scientific name: Methanosarcina sp.
strain designation: MTP4
type strain: no
Morphology
colony morphology
- @ref: 2641
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 2641
- name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141c
- composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Methanol 3.87476 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
- @ref: 2641
- growth: positive
- type: growth
- temperature: 28
Physiology and metabolism
oxygen tolerance
- @ref: 2641
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 2641
- sample type: salt marsh sediment
- geographic location: Bordeaux
- country: France
- origin.country: FRA
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Salt marsh |
| #Environmental | #Terrestrial | #Sediment |
| #Condition | #Saline |
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanosarcina sp. MTP4 | GCA_000970045 | complete | ncbi | 1434100 |
| 66792 | Methanosarcina sp. MTP4 | 2651869869 | complete | img | 1434100 |
| 66792 | Methanosarcina sp. MTP4 | 1434100.4 | complete | patric | 1434100 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 66.176 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 86.929 | yes |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 88.147 | yes |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 84.265 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 86.43 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 82.708 | no |
External links
@ref: 2641
culture collection no.: DSM 6636
straininfo link
- @ref: 76564
- straininfo: 48258
literature
- Pubmed-ID: 16534921
- title: Methanogenic conversion of 3-s-methylmercaptopropionate to 3-mercaptopropionate.
- authors: van der Maarel M, Jansen M, Hansen TA
- journal: Appl Environ Microbiol
- DOI: 10.1128/aem.61.1.48-51.1995
- year: 1995
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 2641 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6636) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6636 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76564 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48258.1 | StrainInfo: A central database for resolving microbial strain identifiers |