Strain identifier

BacDive ID: 7104

Type strain: No

Species: Methanosarcina mazei

Strain Designation: Equi

Strain history: <- OCM <- J. A. G. F. Menaia; Equi {1988}

NCBI tax ID(s): 2209 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2964

BacDive-ID: 7104

DSM-Number: 7058

keywords: Archaea, anaerobe

description: Methanosarcina mazei Equi is an anaerobe archaeon that was isolated from horse caecum.

NCBI tax id

  • NCBI tax id: 2209
  • Matching level: species

strain history

  • @ref: 2964
  • history: <- OCM <- J. A. G. F. Menaia; Equi {1988}

doi: 10.13145/bacdive7104.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina mazei
  • full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
  • synonyms

    @refsynonym
    20215Methanosarcina frisia
    20215Methanococcus frisius
    20215Methanosarcina mazeii
    20215Methanococcus mazei

@ref: 2964

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: Equi

type strain: no

Culture and growth conditions

culture medium

  • @ref: 2964
  • name: METHANOSARCINA MEDIUM (DSMZ Medium 120)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/120
  • composition: Name: METHANOSARCINA MEDIUM (DSMZ Medium 120; with strain-specific modifications) Composition: Trimethylamine-HCl 4.88281 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NaHCO3 1.95312 g/l MgSO4 x 7 H2O 0.488281 g/l NH4Cl 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l MnCl2 x 4 H2O 9.76563e-05 g/l Pyridoxine hydrochloride 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Biotin 1.95312e-05 g/l Folic acid 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

culture temp

  • @ref: 2964
  • growth: positive
  • type: growth
  • temperature: 37

Physiology and metabolism

oxygen tolerance

  • @ref: 2964
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 2964
  • sample type: horse caecum

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body-Site#Gastrointestinal tract#Large intestine

Safety information

risk assessment

  • @ref: 2964
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 2964

culture collection no.: DSM 7058, OCM 61

straininfo link

  • @ref: 76559
  • straininfo: 48352

Reference

@idauthorscataloguedoi/urltitle
2964Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7058)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7058
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
76559Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48352.1StrainInfo: A central database for resolving microbial strain identifiers