Strain identifier

BacDive ID: 7102

Type strain: No

Species: Methanosarcina mazei

Strain history: <- D.R. Boone, LYC

NCBI tax ID(s): 2209 (species)

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General

@ref: 1716

BacDive-ID: 7102

DSM-Number: 4556

keywords: 16S sequence, Archaea, anaerobe

description: Methanosarcina mazei DSM 4556 is an anaerobe archaeon that was isolated from alkaline mud, oil exploration drilling site.

NCBI tax id

  • NCBI tax id: 2209
  • Matching level: species

strain history

  • @ref: 1716
  • history: <- D.R. Boone, LYC

doi: 10.13145/bacdive7102.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina mazei
  • full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

  • synonyms

    @refsynonym
    20215Methanosarcina frisia
    20215Methanococcus frisius
    20215Methanosarcina mazeii
    20215Methanococcus mazei

@ref: 1716

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

type strain: no

Culture and growth conditions

culture medium

  • @ref: 1716
  • name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/120a
  • composition: Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a; with strain-specific modifications) Composition: Methanol 3.86719 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

culture temp

  • @ref: 1716
  • growth: positive
  • type: growth
  • temperature: 37

Physiology and metabolism

oxygen tolerance

  • @ref: 1716
  • oxygen tolerance: anaerobe

compound production

  • @ref: 1716
  • compound: disaggregating enzyme

Isolation, sampling and environmental information

isolation

  • @ref: 1716
  • sample type: alkaline mud, oil exploration drilling site
  • geographic location: Hula swamp area of Galilee
  • country: Israel
  • origin.country: ISR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Oil reservoir
#Environmental#Terrestrial#Mud (Sludge)
#Condition#Alkaline

taxonmaps

  • @ref: 69479
  • File name: preview.99_187097.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_68776;97_89418;98_121696;99_187097&stattab=map
  • Last taxonomy: Methanosarcina
  • 16S sequence: AB065296
  • Sequence Identity:
  • Total samples: 7202
  • soil counts: 2215
  • aquatic counts: 3372
  • animal counts: 684
  • plant counts: 931

Safety information

risk assessment

  • @ref: 1716
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Methanosarcina mazei gene for 16S rRNA
  • accession: AB065296
  • length: 1439
  • database: nuccore
  • NCBI tax ID: 2209

External links

@ref: 1716

culture collection no.: DSM 4556, ATCC 43573, OCM 34

straininfo link

  • @ref: 76557
  • straininfo: 47856

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1716Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4556)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4556
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76557Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47856.1StrainInfo: A central database for resolving microbial strain identifiers