Strain identifier
BacDive ID: 7102
Type strain: 
Species: Methanosarcina mazei
Strain history: <- D.R. Boone, LYC
NCBI tax ID(s): 2209 (species)
version 8.1 (current version)
General
@ref: 1716
BacDive-ID: 7102
DSM-Number: 4556
keywords: 16S sequence, Archaea, anaerobe, mesophilic
description: Methanosarcina mazei DSM 4556 is an anaerobe, mesophilic archaeon that was isolated from alkaline mud, oil exploration drilling site.
NCBI tax id
- NCBI tax id: 2209
- Matching level: species
strain history
- @ref: 1716
- history: <- D.R. Boone, LYC
doi: 10.13145/bacdive7102.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina mazei
- full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
synonyms
@ref synonym 20215 Methanosarcina frisia 20215 Methanococcus frisius 20215 Methanosarcina mazeii 20215 Methanococcus mazei
@ref: 1716
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina mazei
full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984
type strain: no
Culture and growth conditions
culture medium
- @ref: 1716
- name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/120a
- composition: Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a; with strain-specific modifications) Composition: Methanol 3.86719 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
culture temp
- @ref: 1716
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1716
- oxygen tolerance: anaerobe
compound production
- @ref: 1716
- compound: disaggregating enzyme
Isolation, sampling and environmental information
isolation
- @ref: 1716
- sample type: alkaline mud, oil exploration drilling site
- geographic location: Hula swamp area of Galilee
- country: Israel
- origin.country: ISR
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Oil reservoir |
| #Environmental | #Terrestrial | #Mud (Sludge) |
| #Condition | #Alkaline |
taxonmaps
- @ref: 69479
- File name: preview.99_187097.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_68776;97_89418;98_121696;99_187097&stattab=map
- Last taxonomy: Methanosarcina
- 16S sequence: AB065296
- Sequence Identity:
- Total samples: 7202
- soil counts: 2215
- aquatic counts: 3372
- animal counts: 684
- plant counts: 931
Safety information
risk assessment
- @ref: 1716
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Methanosarcina mazei gene for 16S rRNA | AB065296 | 1439 | ena | 2209 |
| 20218 | Lactobacillus paracasei clone OCM034 16S ribosomal RNA gene, partial sequence | GU425032 | 469 | ena | 1597 |
External links
@ref: 1716
culture collection no.: DSM 4556, ATCC 43573, OCM 34
straininfo link
- @ref: 76557
- straininfo: 47856
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1716 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4556) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4556 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 76557 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47856.1 | StrainInfo: A central database for resolving microbial strain identifiers |