General

@ref: 1358

BacDive-ID: 7100

DSM-Number: 3338

keywords: Archaea, anaerobe

description: Methanosarcina mazei G1 is an anaerobe archaeon that was isolated from sewage mud.

NCBI tax id

• NCBI tax id: 2209
• Matching level: species

strain history

• @ref: 1358
• history: <- M. P. Bryant, University of Illinois, Departments of Dairy Science and Microbiology, Urbana, USA; G1 <- M. P. Bryant {1966}

doi: 10.13145/bacdive7100.20240510.9

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanosarcinaceae
• genus: Methanosarcina
• species: Methanosarcina mazei
• full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

• synonyms

  @ref	synonym
  20215	Methanosarcina frisia
  20215	Methanococcus frisius
  20215	Methanosarcina mazeii
  20215	Methanococcus mazei

@ref: 1358

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: G1

type strain: no

Culture and growth conditions

culture medium

• @ref: 1358
• name: METHANOSARCINA MEDIUM (DSMZ Medium 120)
• growth: yes
• link: https://mediadive.dsmz.de/medium/120
• composition: Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

culture temp

• @ref: 1358
• growth: positive
• type: growth
• temperature: 35

Physiology and metabolism

oxygen tolerance

• @ref: 1358
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 1358
• sample type: sewage mud
• geographic location: Göttingen
• country: Germany
• origin.country: DEU
• continent: Europe

isolation source categories

Safety information

risk assessment

• @ref: 1358
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

GC content

• @ref: 1358
• GC-content: 36.1

External links

@ref: 1358

culture collection no.: DSM 3338, OCM 87

straininfo link

• @ref: 76555
• straininfo: 47717

Reference