Strain identifier

BacDive ID: 7099

Type strain: No

Species: Methanosarcina mazei

Strain Designation: C16

Strain history: <- K.H. Blotevogel, C16

NCBI tax ID(s): 2209 (species)

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General

@ref: 1355

BacDive-ID: 7099

DSM-Number: 3318

keywords: genome sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina mazei C16 is an anaerobe, mesophilic archaeon that was isolated from marine sediment.

NCBI tax id

  • NCBI tax id: 2209
  • Matching level: species

strain history

  • @ref: 1355
  • history: <- K.H. Blotevogel, C16

doi: 10.13145/bacdive7099.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina mazei
  • full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

  • synonyms

    @refsynonym
    20215Methanosarcina frisia
    20215Methanococcus frisius
    20215Methanosarcina mazeii
    20215Methanococcus mazei

@ref: 1355

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: C16

type strain: no

Culture and growth conditions

culture medium

  • @ref: 1355
  • name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/141c
  • composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Methanol 3.87476 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

  • @ref: 1355
  • growth: positive
  • type: growth
  • temperature: 35
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1355
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1355
  • sample type: marine sediment
  • geographic location: Greetsiel, North Sea
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

Safety information

risk assessment

  • @ref: 1355
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanosarcina mazei C16GCA_000970245completencbi1434113
66792Methanosarcina mazei C162636415905completeimg1434113
66792Methanosarcina mazei C161434113.4completepatric1434113

GC content

  • @ref: 1355
  • GC-content: 38.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno91.442no
flagellatedno97.205no
gram-positiveno95.818no
anaerobicyes97.538yes
aerobicno95.038no
halophileno89.293no
spore-formingno95.435no
thermophileno97.6yes
glucose-utilyes69.008no
glucose-fermentno71.785no

External links

@ref: 1355

culture collection no.: DSM 3318, ATCC 43340, OCM 98

straininfo link

  • @ref: 76554
  • straininfo: 132872

Reference

@idauthorscataloguedoi/urltitle
1355Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3318)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3318
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76554Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132872.1StrainInfo: A central database for resolving microbial strain identifiers