Strain identifier

BacDive ID: 7098

Type strain: No

Species: Methanosarcina mazei

Strain Designation: MC3

Strain history: <- J.P. Touzel, MC3 (INRA)

NCBI tax ID(s): 2209 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 1240

BacDive-ID: 7098

DSM-Number: 2907

keywords: Archaea, anaerobe, mesophilic

description: Methanosarcina mazei MC3 is an anaerobe, mesophilic archaeon that was isolated from digester sludge.

NCBI tax id

  • NCBI tax id: 2209
  • Matching level: species

strain history

  • @ref: 1240
  • history: <- J.P. Touzel, MC3 (INRA)

doi: 10.13145/bacdive7098.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina mazei
  • full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
  • synonyms

    @refsynonym
    20215Methanosarcina frisia
    20215Methanococcus frisius
    20215Methanosarcina mazeii
    20215Methanococcus mazei

@ref: 1240

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: MC3

type strain: no

Culture and growth conditions

culture medium

  • @ref: 1240
  • name: METHANOSARCINA THERMOPHILA (BCYT) MEDIUM (DSMZ Medium 318)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/318
  • composition: Name: METHANOSARCINA THERMOPHILA (BCYT) MEDIUM (DSMZ Medium 318) Composition: Methanol 7.7571 g/l KHCO3 1.95886 g/l NH4Cl 0.979432 g/l Yeast extract 0.489716 g/l Trypticase peptone 0.489716 g/l Na2S x 9 H2O 0.29383 g/l KH2PO4 0.29383 g/l L-Cysteine HCl x H2O 0.29383 g/l Nitrilotriacetic acid 0.125367 g/l MgCl2 x 6 H2O 0.0979432 g/l NaCl 0.00979432 g/l FeCl2 x 4 H2O 0.00979432 g/l CaCl2 x 2 H2O 0.000979432 g/l MnCl2 x 4 H2O 0.000979432 g/l NiCl2 x 6 H2O 0.000979432 g/l ZnCl2 0.000979432 g/l Sodium resazurin 0.000489716 g/l Na2WO4 x 2 H2O 0.000391773 g/l Na2MoO4 x 2 H2O 0.00029383 g/l CoCl2 x 6 H2O 0.00029383 g/l Na2SeO3 x 5 H2O 0.00029383 g/l CuCl2 0.000195886 g/l H3BO3 9.79432e-05 g/l Pyridoxine hydrochloride 9.79432e-05 g/l Calcium D-(+)-pantothenate 4.89716e-05 g/l (DL)-alpha-Lipoic acid 4.89716e-05 g/l p-Aminobenzoic acid 4.89716e-05 g/l Nicotinic acid 4.89716e-05 g/l Riboflavin 4.89716e-05 g/l Thiamine HCl 4.89716e-05 g/l Folic acid 1.95886e-05 g/l Biotin 1.95886e-05 g/l Vitamin B12 9.79432e-07 g/l Distilled water

culture temp

  • @ref: 1240
  • growth: positive
  • type: growth
  • temperature: 35
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1240
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1240
  • sample type: digester sludge
  • geographic location: Lille
  • country: France
  • origin.country: FRA
  • continent: Europe

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Sewage sludge

Safety information

risk assessment

  • @ref: 1240
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 1240

culture collection no.: DSM 2907, OCM 192

straininfo link

  • @ref: 76553
  • straininfo: 47669

Reference

@idauthorscataloguedoi/urltitle
1240Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2907)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2907
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
76553Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47669.1StrainInfo: A central database for resolving microbial strain identifiers