Strain identifier

BacDive ID: 7089

Type strain: No

Species: Methanosarcina sp.

Strain Designation: 3

Strain history: <- H. Hippe, 3

NCBI tax ID(s): 2213 (species)

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General

@ref: 461

BacDive-ID: 7089

DSM-Number: 805

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina sp. 3 is an anaerobe, mesophilic archaeon that was isolated from ditch mud.

NCBI tax id

  • NCBI tax id: 2213
  • Matching level: species

strain history

  • @ref: 461
  • history: <- H. Hippe, 3

doi: 10.13145/bacdive7089.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina sp.
  • full scientific name: Methanosarcina Kluyver and van Niel 1936 (Approved Lists 1980)

@ref: 461

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina sp.

full scientific name: Methanosarcina sp.

strain designation: 3

type strain: no

Culture and growth conditions

culture medium

  • @ref: 461
  • name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/120a
  • composition: Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) Composition: Methanol 15.4688 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NaHCO3 0.830078 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

culture temp

  • @ref: 461
  • growth: positive
  • type: growth
  • temperature: 35
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 461
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 461
  • sample type: ditch mud
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Mud (Sludge)

Sequence information

16S sequences

  • @ref: 20218
  • description: Enterococcus faecalis clone OCM084 16S ribosomal RNA gene, partial sequence
  • accession: GU417253
  • length: 454
  • database: ena
  • NCBI tax ID: 1351

External links

@ref: 461

culture collection no.: DSM 805, ATCC 29786, OCM 84

straininfo link

  • @ref: 76544
  • straininfo: 39719

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
461Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 805)https://www.dsmz.de/collection/catalogue/details/culture/DSM-805
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
76544Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39719.1StrainInfo: A central database for resolving microbial strain identifiers