General

@ref: 1274

BacDive-ID: 7083

DSM-Number: 3028

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina siciliae T4/M is an anaerobe, mesophilic archaeon that was isolated from lake sediment.

NCBI tax id

• NCBI tax id: 38027
• Matching level: species

strain history

• @ref: 1274
• history: <- K.O. Stetter, T4/M

doi: 10.13145/bacdive7083.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanosarcinaceae
• genus: Methanosarcina
• species: Methanosarcina siciliae
• full scientific name: Methanosarcina siciliae (Stetter and König 1989) Ni et al. 1994

• synonyms

  • @ref: 20215
  • synonym: Methanolobus siciliae

@ref: 1274

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina siciliae

full scientific name: Methanosarcina siciliae (Stetter and König 1989) Ni et al. 1994 emend. Elberson and Sowers 1997

strain designation: T4/M

type strain: yes

Culture and growth conditions

culture medium

• @ref: 1274
• name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141c
• composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Methanol 3.87476 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

• @ref: 1274
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 1274
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 1274
• sample type: lake sediment
• geographic location: Sicily, Lakes of Marinello
• country: Italy
• origin.country: ITA
• continent: Europe

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_187088.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_68776;97_89418;98_121687;99_187088&stattab=map
• Last taxonomy: Methanosarcina siciliae
• 16S sequence: FR733698
• Sequence Identity:
• Total samples: 286
• soil counts: 112
• aquatic counts: 149
• animal counts: 15
• plant counts: 10

Safety information

risk assessment

• @ref: 1274
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 1274
• description: Methanosarcina siciliae partial 16S rRNA gene, type strain DSM3028T
• accession: FR733698
• length: 1472
• database: ena
• NCBI tax ID: 38027

Genome sequences

GC content

• @ref: 1274
• GC-content: 41.5

Genome-based predictions

predictions

External links

@ref: 1274

culture collection no.: DSM 3028, OCM 156

straininfo link

• @ref: 76539
• straininfo: 47685

literature

Reference