Strain identifier
BacDive ID: 7081
Type strain:
Species: Methanolobus profundi
Strain Designation: MobM
Strain history: <- H. Mochimaru, Natl. Inst. Advanced Industrial Sci. and Technol., Tsukuba, Japan; MobM <- H. Mochimaru {2003}
NCBI tax ID(s): 487685 (species)
General
@ref: 15580
BacDive-ID: 7081
DSM-Number: 21213
keywords: 16S sequence, Archaea, anaerobe, mesophilic
description: Methanolobus profundi MobM is an anaerobe, mesophilic archaeon that was isolated from deep subsurface sediments from a natural gas field filled with ancient seawater.
NCBI tax id
- NCBI tax id: 487685
- Matching level: species
strain history
- @ref: 15580
- history: <- H. Mochimaru, Natl. Inst. Advanced Industrial Sci. and Technol., Tsukuba, Japan; MobM <- H. Mochimaru {2003}
doi: 10.13145/bacdive7081.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanolobus
- species: Methanolobus profundi
- full scientific name: Methanolobus profundi Mochimaru et al. 2009
@ref: 15580
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanolobus
species: Methanolobus profundi
full scientific name: Methanolobus profundi Mochimaru et al. 2009
strain designation: MobM
type strain: yes
Culture and growth conditions
culture medium
- @ref: 15580
- name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141c
- composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Methanol 3.87476 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
- @ref: 15580
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 15580
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 15580
- sample type: deep subsurface sediments from a natural gas field filled with ancient seawater
- geographic location: Mobara
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Sediment
taxonmaps
- @ref: 69479
- File name: preview.99_188632.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_69178;97_89866;98_122820;99_188632&stattab=map
- Last taxonomy: Methanolobus profundi
- 16S sequence: AB370245
- Sequence Identity:
- Total samples: 5
- soil counts: 1
- aquatic counts: 3
- plant counts: 1
Safety information
risk assessment
- @ref: 15580
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15580
- description: Methanolobus profundi gene for 16S ribosomal RNA, partial sequence
- accession: AB370245
- length: 1403
- database: ena
- NCBI tax ID: 487685
GC content
- @ref: 15580
- GC-content: 42.4
- method: high performance liquid chromatography (HPLC)
External links
@ref: 15580
culture collection no.: DSM 21213, NBRC 104158
straininfo link
- @ref: 76537
- straininfo: 397727
literature
- topic: Phylogeny
- Pubmed-ID: 19329594
- title: Methanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field.
- authors: Mochimaru H, Tamaki H, Hanada S, Imachi H, Nakamura K, Sakata S, Kamagata Y
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.001677-0
- year: 2009
- mesh: Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Geologic Sediments/*microbiology, Japan, Magnesium/metabolism, Methane/*metabolism, Methanol/metabolism, Methanosarcinaceae/*classification/genetics/*isolation & purification/metabolism, Methylamines/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium/metabolism
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
15580 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21213) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21213 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
76537 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID397727.1 | StrainInfo: A central database for resolving microbial strain identifiers |