Strain identifier

BacDive ID: 7081

Type strain: Yes

Species: Methanolobus profundi

Strain Designation: MobM

Strain history: <- H. Mochimaru, Natl. Inst. Advanced Industrial Sci. and Technol., Tsukuba, Japan; MobM <- H. Mochimaru {2003}

NCBI tax ID(s): 487685 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 15580

BacDive-ID: 7081

DSM-Number: 21213

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanolobus profundi MobM is an anaerobe, mesophilic archaeon that was isolated from deep subsurface sediments from a natural gas field filled with ancient seawater.

NCBI tax id

  • NCBI tax id: 487685
  • Matching level: species

strain history

  • @ref: 15580
  • history: <- H. Mochimaru, Natl. Inst. Advanced Industrial Sci. and Technol., Tsukuba, Japan; MobM <- H. Mochimaru {2003}

doi: 10.13145/bacdive7081.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanolobus
  • species: Methanolobus profundi
  • full scientific name: Methanolobus profundi Mochimaru et al. 2009

@ref: 15580

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanolobus

species: Methanolobus profundi

full scientific name: Methanolobus profundi Mochimaru et al. 2009

strain designation: MobM

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 15580
  • name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/141c
  • composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Methanol 3.87476 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

  • @ref: 15580
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 15580
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 15580
  • sample type: deep subsurface sediments from a natural gas field filled with ancient seawater
  • geographic location: Mobara
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_188632.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_69178;97_89866;98_122820;99_188632&stattab=map
  • Last taxonomy: Methanolobus profundi
  • 16S sequence: AB370245
  • Sequence Identity:
  • Total samples: 5
  • soil counts: 1
  • aquatic counts: 3
  • plant counts: 1

Safety information

risk assessment

  • @ref: 15580
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15580
  • description: Methanolobus profundi gene for 16S ribosomal RNA, partial sequence
  • accession: AB370245
  • length: 1403
  • database: ena
  • NCBI tax ID: 487685

GC content

  • @ref: 15580
  • GC-content: 42.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 15580

culture collection no.: DSM 21213, NBRC 104158

straininfo link

  • @ref: 76537
  • straininfo: 397727

literature

  • topic: Phylogeny
  • Pubmed-ID: 19329594
  • title: Methanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field.
  • authors: Mochimaru H, Tamaki H, Hanada S, Imachi H, Nakamura K, Sakata S, Kamagata Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.001677-0
  • year: 2009
  • mesh: Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Geologic Sediments/*microbiology, Japan, Magnesium/metabolism, Methane/*metabolism, Methanol/metabolism, Methanosarcinaceae/*classification/genetics/*isolation & purification/metabolism, Methylamines/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium/metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
15580Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21213)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21213
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76537Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID397727.1StrainInfo: A central database for resolving microbial strain identifiers