Archive BacDiveID:10.13145/bacdive7076.20230509.8.1

Strain identifier

BacDive ID: 7076

Type strain:

Species: Methanolobus tindarius

Strain history: <- K.O. Stetter, Tindari 3

NCBI tax ID(s): 1090322 (strain), 2221 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 1013

BacDive-ID: 7076

DSM-Number: 2278

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanolobus tindarius DSM 2278 is an anaerobe, mesophilic archaeon that was isolated from sediment.

NCBI tax id

NCBI tax id	Matching level
1090322	strain
2221	species

strain history

@ref: 1013
history: <- K.O. Stetter, Tindari 3

doi: 10.13145/bacdive7076.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/methanobacteriota
domain: Archaea
phylum: Methanobacteriota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanolobus
species: Methanolobus tindarius
full scientific name: Methanolobus tindarius König and Stetter 1983

@ref: 1013

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanolobus

species: Methanolobus tindarius

full scientific name: Methanolobus tindarius König and Stetter 1983

type strain: yes

Culture and growth conditions

culture medium

@ref: 1013
name: METHANOLOBUS TINDARIUS MEDIUM (DSMZ Medium 233)
growth: yes
link: https://mediadive.dsmz.de/medium/233
composition: Name: METHANOLOBUS TINDARIUS MEDIUM (DSMZ Medium 233) Composition: Methanol 7.74194 g/l MgCl2 x 6 H2O 3.91007 g/l NaHCO3 0.977517 g/l Na2S x 9 H2O 0.488759 g/l L-Cysteine HCl x H2O 0.488759 g/l KCl 0.332356 g/l NH4Cl 0.244379 g/l K2HPO4 0.136852 g/l MgSO4 x 7 H2O 0.0293255 g/l Nitrilotriacetic acid 0.0146628 g/l NaCl 0.00977517 g/l MnSO4 x H2O 0.00488759 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195503 g/l CoSO4 x 7 H2O 0.00175953 g/l ZnSO4 x 7 H2O 0.00175953 g/l CaCl2 x 2 H2O 0.000977517 g/l FeSO4 x 7 H2O 0.000977517 g/l Sodium resazurin 0.000488759 g/l NiCl2 x 6 H2O 0.000293255 g/l AlK(SO4)2 x 12 H2O 0.000195503 g/l CuSO4 x 5 H2O 9.77517e-05 g/l Na2MoO4 x 2 H2O 9.77517e-05 g/l Pyridoxine hydrochloride 9.77517e-05 g/l H3BO3 9.77517e-05 g/l (DL)-alpha-Lipoic acid 4.88759e-05 g/l Calcium D-(+)-pantothenate 4.88759e-05 g/l p-Aminobenzoic acid 4.88759e-05 g/l Nicotinic acid 4.88759e-05 g/l Riboflavin 4.88759e-05 g/l Thiamine HCl 4.88759e-05 g/l Folic acid 1.95503e-05 g/l Biotin 1.95503e-05 g/l Na2WO4 x 2 H2O 3.91007e-06 g/l Na2SeO3 x 5 H2O 2.93255e-06 g/l Vitamin B12 9.77517e-07 g/l Distilled water

culture temp

@ref: 1013
growth: positive
type: growth
temperature: 25
range: mesophilic

Physiology and metabolism

oxygen tolerance

@ref: 1013
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref: 1013
sample type: sediment
geographic location: Lakes of Marinello near Tindari
country: Italy
origin.country: ITA
continent: Europe

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Sediment

Safety information

risk assessment

@ref: 1013
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 1013
description: Methanolobus tindarius 16S ribosomal RNA gene, partial sequence
accession: M59135
length: 1422
database: ena
NCBI tax ID: 2221

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Methanolobus tindarius DSM 2278	GCA_000504205	contig	ncbi	1090322
66792	Methanolobus tindarius DSM 2278	2515075008	draft	img	1090322
66792	Methanolobus tindarius DSM 2278	1090322.3	wgs	patric	1090322

GC content

@ref: 1013
GC-content: 46.0

External links

@ref: 1013

culture collection no.: DSM 2278, ATCC 35996, OCM 150, Tindari 3

straininfo link

@ref: 76532
straininfo: 41410

literature

topic: Phylogeny
Pubmed-ID: 19329594
title: Methanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field.
authors: Mochimaru H, Tamaki H, Hanada S, Imachi H, Nakamura K, Sakata S, Kamagata Y
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.001677-0
year: 2009
mesh: Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Geologic Sediments/*microbiology, Japan, Magnesium/metabolism, Methane/*metabolism, Methanol/metabolism, Methanosarcinaceae/*classification/genetics/*isolation & purification/metabolism, Methylamines/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium/metabolism
topic2: Metabolism

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
1013	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2278)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-2278
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg		10.1093/nar/gkaa1025	BRENDA, the ELIXIR core data resource in 2021: new developments and updates	Nucleic Acids Res. 49: D498-D508 2020	33211880
76532	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID41410.1	StrainInfo: A central database for resolving microbial strain identifiers