Strain identifier
BacDive ID: 7068
Type strain: 
Species: Methanohalophilus mahii
Strain Designation: SLP
Strain history: <- OCM <- I.M. Mathrani <- ATCC <- J.R. Paterek, SLP
NCBI tax ID(s): 547558 (strain), 2176 (species)
version 8.1 (current version)
General
@ref: 1965
BacDive-ID: 7068
DSM-Number: 5219
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic, Gram-negative
description: Methanohalophilus mahii SLP is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from anaerobic sediment.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 2176 | species |
| 547558 | strain |
strain history
- @ref: 1965
- history: <- OCM <- I.M. Mathrani <- ATCC <- J.R. Paterek, SLP
doi: 10.13145/bacdive7068.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanohalophilus
- species: Methanohalophilus mahii
- full scientific name: Methanohalophilus mahii Paterek and Smith 1988
@ref: 1965
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanohalophilus
species: Methanohalophilus mahii
full scientific name: Methanohalophilus mahii Paterek and Smith 1988
strain designation: SLP
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.988
multimedia
- @ref: 66793
- multimedia content: EM_DSM_5219_1.jpg
- caption: electron microscopic image
- intellectual property rights: © HZI/Manfred Rohde
Culture and growth conditions
culture medium
- @ref: 1965
- name: METHANOHALOPHILUS MEDIUM (DSMZ Medium 479)
- growth: yes
- link: https://mediadive.dsmz.de/medium/479
- composition: Name: METHANOHALOPHILUS MEDIUM (DSMZ Medium 479; with strain-specific modifications) Composition: MgCl2 x 6 H2O 6.0 g/l Trimethylamine-HCl 2.0 g/l Trypticase peptone 2.0 g/l Yeast extract 2.0 g/l KCl 1.5 g/l Na2CO3 1.5 g/l NH4Cl 1.0 g/l Casamino acids 0.5 g/l K2HPO4 x 3 H2O 0.4 g/l Na2S x 9 H2O 0.25 g/l 2-Mercaptoethanesulfonic acid 0.2 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water
culture temp
- @ref: 1965
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 1965 | anaerobe | |
| 69480 | anaerobe | 99.096 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.897 |
Isolation, sampling and environmental information
isolation
- @ref: 1965
- sample type: anaerobic sediment
- geographic location: Utah, Great Salt Lake
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Sediment |
| #Condition | #Anoxic (anaerobic) |
Safety information
risk assessment
- @ref: 1965
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Enterococcus faecalis clone OCM068 16S ribosomal RNA gene, partial sequence | GU417244 | 456 | ena | 1351 |
| 1965 | Methanohalophilus mahii DSM 5219 16S ribosomal RNA gene, partial sequence | M59133 | 1421 | ena | 547558 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanohalophilus mahii DSM 5219 | GCA_000025865 | complete | ncbi | 547558 |
| 66792 | Methanohalophilus mahii SLP, DSM 5219 | 646564550 | complete | img | 547558 |
| 66792 | Methanohalophilus mahii DSM 5219 | 547558.18 | complete | patric | 547558 |
GC content
- @ref: 1965
- GC-content: 48.5
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 92.985 | no |
| flagellated | no | 97.449 | no |
| gram-positive | no | 96.587 | no |
| anaerobic | yes | 97.909 | no |
| aerobic | no | 93.153 | yes |
| halophile | no | 66.799 | no |
| spore-forming | no | 96.254 | no |
| thermophile | no | 76.015 | no |
| glucose-util | yes | 76.761 | no |
| glucose-ferment | no | 73.631 | no |
External links
@ref: 1965
culture collection no.: DSM 5219, ATCC 35705, OCM 68
straininfo link
- @ref: 76525
- straininfo: 41239
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1965 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5219) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5219 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 76525 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41239.1 | StrainInfo: A central database for resolving microbial strain identifiers |