Strain identifier
BacDive ID: 7054
Type strain: 
Species: Methanomethylovorans thermophila
Strain Designation: L2FAW
Strain history: <- B. Jiang, Wageningen University, Laboratory of Microbiology, Netherlands; L2FAW <- B. Jiang {2003}
NCBI tax ID(s): 286152 (species)
version 8.1 (current version)
General
@ref: 6828
BacDive-ID: 7054
DSM-Number: 17232
keywords: 16S sequence, Archaea, anaerobe, thermophilic
description: Methanomethylovorans thermophila L2FAW is an anaerobe, thermophilic archaeon that was isolated from thermophilic sludge of pilot bioreactor.
NCBI tax id
- NCBI tax id: 286152
- Matching level: species
strain history
- @ref: 6828
- history: <- B. Jiang, Wageningen University, Laboratory of Microbiology, Netherlands; L2FAW <- B. Jiang {2003}
doi: 10.13145/bacdive7054.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanomethylovorans
- species: Methanomethylovorans thermophila
- full scientific name: Methanomethylovorans thermophila Jiang et al. 2005
@ref: 6828
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanomethylovorans
species: Methanomethylovorans thermophila
full scientific name: Methanomethylovorans thermophila Jiang et al. 2005
strain designation: L2FAW
type strain: yes
Morphology
colony morphology
- @ref: 6828
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 6828
- name: SYNTROPHOBACTER (MPOB) MEDIUM (DSMZ Medium 684)
- growth: yes
- link: https://mediadive.dsmz.de/medium/684
- composition: Name: SYNTROPHOBACTER (MPOB) MEDIUM (DSMZ Medium 684; with strain-specific modifications) Composition: Methanol 1.57926 g/l Na2CO3 1.49551 g/l Na2HPO4 x 2 H2O 0.528415 g/l Na2S x 9 H2O 0.498504 g/l KH2PO4 0.408774 g/l NH4Cl 0.299103 g/l NaCl 0.299103 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.109671 g/l MgCl2 x 6 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
- @ref: 6828
- growth: positive
- type: growth
- temperature: 50
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 6828
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 6828
- sample type: thermophilic sludge of pilot bioreactor
- geographic location: Balk
- country: Netherlands
- origin.country: NLD
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Bioreactor | |
| #Environmental | #Terrestrial | #Mud (Sludge) |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_188529.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_68856;97_89796;98_122108;99_188529&stattab=map
- Last taxonomy: Methanomethylovorans thermophila
- 16S sequence: AY672821
- Sequence Identity:
- Total samples: 11
- aquatic counts: 10
- animal counts: 1
Safety information
risk assessment
- @ref: 6828
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6828
- description: Methanomethylovorans thermophila 16S ribosomal RNA gene, partial sequence
- accession: AY672821
- length: 1363
- database: ena
- NCBI tax ID: 286152
GC content
- @ref: 6828
- GC-content: 37.6
External links
@ref: 6828
culture collection no.: DSM 17232, ATCC BAA 1173, NBRC 107638
straininfo link
- @ref: 76511
- straininfo: 281453
literature
- topic: Phylogeny
- Pubmed-ID: 16280511
- title: Methanomethylovorans thermophila sp. nov., a thermophilic, methylotrophic methanogen from an anaerobic reactor fed with methanol.
- authors: Jiang B, Parshina SN, van Doesburg W, Lomans BP, Stams AJM
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63818-0
- year: 2005
- mesh: Anaerobiosis, Base Sequence, Bioreactors/microbiology, Methanol/*metabolism, Methanosarcinaceae/genetics/growth & development/*isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis/genetics, Sulfides/*metabolism, Temperature
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 6828 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17232) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17232 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 76511 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID281453.1 | StrainInfo: A central database for resolving microbial strain identifiers |