Strain identifier

BacDive ID: 7048

Type strain: No

Species: Methanothrix thermoacetophila

Strain Designation: CALS-1

Strain history: <- S. H. Zinder, Cornell University, Ithaca, NY, USA; CALS-1

NCBI tax ID(s): 2224 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1490

BacDive-ID: 7048

DSM-Number: 3870

keywords: 16S sequence, Archaea, anaerobe

description: Methanothrix thermoacetophila CALS-1 is an anaerobe archaeon that was isolated from thermophilic anaerobic bioreactor.

NCBI tax id

  • NCBI tax id: 2224
  • Matching level: species

strain history

  • @ref: 1490
  • history: <- S. H. Zinder, Cornell University, Ithaca, NY, USA; CALS-1

doi: 10.13145/bacdive7048.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanotrichaceae
  • genus: Methanothrix
  • species: Methanothrix thermoacetophila
  • full scientific name: Methanothrix thermoacetophila corrig. Nozhevnikova and Chudina 1988
  • synonyms

    @refsynonym
    20215Methanothrix thermophila
    20215Methanosaeta thermophila
    20215Methanothrix thermoacetophilia
    20215Methanosaeta thermoacetophila

@ref: 1490

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosaetaceae

genus: Methanothrix

species: Methanothrix thermoacetophila

full scientific name: Methanosaeta thermophila (Kamagata et al. 1992) Boone and Kamagata 1998

strain designation: CALS-1

type strain: no

Morphology

colony morphology

  • @ref: 1490
  • incubation period: 3-7 days

Physiology and metabolism

oxygen tolerance

  • @ref: 1490
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1490
  • sample type: thermophilic anaerobic bioreactor
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2
#Engineered#Bioreactor
#Condition#Anoxic (anaerobic)
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 1490
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Methanothrix thermophila 16S ribosomal RNA gene, partial sequenceM591411422nuccore2224
1490Methanothrix thermoacetophila strain CALS-1 16S ribosomal RNA, partial sequenceAH003995739nuccore2224

External links

@ref: 1490

culture collection no.: DSM 3870

straininfo link

  • @ref: 76507
  • straininfo: 47769

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1380291Characterization of three thermophilic strains of Methanothrix ("Methanosaeta") thermophila sp. nov. and rejection of Methanothrix ("Methanosaeta") thermoacetophila.Kamagata Y, Kawasaki H, Oyaizu H, Nakamura K, Mikami E, Endo G, Koga Y, Yamasato KInt J Syst Bacteriol10.1099/00207713-42-3-4631992Bacterial Proteins/chemistry, Base Composition, Base Sequence, DNA, Bacterial/chemistry, Electrophoresis, Polyacrylamide Gel, Methanosarcinaceae/*classification/cytology/physiology, Molecular Sequence Data, RNA, Bacterial/chemistry, RNA, Ribosomal, 16S/chemistry, Sequence Homology, Nucleic Acid, Terminology as TopicEnzymology
8824151Methanogenesis from acetate by cell-free extracts of the thermophilic acetotrophic methanogen Methanothrix thermophila CALS-1Allen GWJ, Zinder SHArch Microbiol10.1007/s0020300503841996

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1490Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3870)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3870
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
76507Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47769.1StrainInfo: A central database for resolving microbial strain identifiers