Strain identifier

BacDive ID: 7043

Type strain: Yes

Species: Methanolacinia petrolearia

Strain history: <- B. Ollivier, Université de Provence, Marseille, France; SEBR 4847 <- B. Ollivier {1991}

NCBI tax ID(s): 679926 (strain), 54120 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4325

BacDive-ID: 7043

DSM-Number: 11571

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic, Gram-negative

description: Methanolacinia petrolearia DSM 11571 is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from offshore oil field.

NCBI tax id

NCBI tax idMatching level
54120species
679926strain

strain history

  • @ref: 4325
  • history: <- B. Ollivier, Université de Provence, Marseille, France; SEBR 4847 <- B. Ollivier {1991}

doi: 10.13145/bacdive7043.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanomicrobiales
  • family: Methanomicrobiaceae
  • genus: Methanolacinia
  • species: Methanolacinia petrolearia
  • full scientific name: Methanolacinia petrolearia (Ollivier et al. 1998) Göker et al. 2015
  • synonyms

    • @ref: 20215
    • synonym: Methanoplanus petrolearius

@ref: 4325

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanomicrobiaceae

genus: Methanolacinia

species: Methanolacinia petrolearia

full scientific name: Methanolacinia petrolearia (Ollivier et al. 1998) Göker et al. 2015

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.993

multimedia

  • @ref: 66793
  • multimedia content: EM_DSM_11571_1.jpg
  • caption: electron microscopic image
  • intellectual property rights: © HZI/Manfred Rohde

Culture and growth conditions

culture medium

  • @ref: 4325
  • name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/141
  • composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

  • @ref: 4325
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
4325anaerobe
69480anaerobe99.448

spore formation

@refspore formationconfidence
69481no100
69480no99.779

Isolation, sampling and environmental information

isolation

  • @ref: 4325
  • sample type: offshore oil field
  • geographic location: Western African Coast, Gulf of Guinea

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Oil reservoir
#Environmental#Aquatic#Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_187192.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18124;96_68849;97_89495;98_121775;99_187192&stattab=map
  • Last taxonomy: Methanolacinia
  • 16S sequence: U76631
  • Sequence Identity:
  • Total samples: 285
  • soil counts: 47
  • aquatic counts: 198
  • animal counts: 4
  • plant counts: 36

Safety information

risk assessment

  • @ref: 4325
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4325
  • description: Methanoplanus petrolearius 16S ribosomal RNA gene, complete sequence
  • accession: U76631
  • length: 1429
  • database: ena
  • NCBI tax ID: 679926

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanolacinia petrolearia DSM 11571GCA_000147875completencbi679926
66792Methanolacinia petrolearia DSM 11571648028040completeimg679926
66792Methanoplanus petrolearius DSM 11571679926.6completepatric679926

GC content

@refGC-contentmethod
432547sequence analysis
432550high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno90.936no
flagellatedno97.024no
gram-positiveno96.342no
anaerobicyes99.278yes
aerobicno95.289yes
halophileno82.218no
spore-formingno95.872no
thermophileno91.296yes
glucose-utilyes74.233no
glucose-fermentno68.81no

External links

@ref: 4325

culture collection no.: DSM 11571, OCM 486, SEBR 4847

straininfo link

  • @ref: 76502
  • straininfo: 49139

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9037763Methanoplanus petrolearius sp. nov., a novel methanogenic bacterium from an oil-producing well.Ollivier B, Cayol JL, Patel BK, Magot M, Fardeau ML, Garcia JLFEMS Microbiol Lett10.1111/j.1574-6968.1997.tb10219.x1997Bacteriological Techniques, Classification, Culture Media, Cytosine, DNA, Bacterial/analysis, Euryarchaeota/*classification/cytology/*isolation & purification, Guanine, Guinea, Molecular Sequence Data, Oils, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, Sequence Analysis, RNA, *Soil MicrobiologyCultivation
Genetics21304750Complete genome sequence of Methanoplanus petrolearius type strain (SEBR 4847).Brambilla E, Djao OD, Daligault H, Lapidus A, Lucas S, Hammon N, Nolan M, Tice H, Cheng JF, Han C, Tapia R, Goodwin L, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Rohde M, Spring S, Sikorski J, Goker M, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HPStand Genomic Sci10.4056/sigs.11831432010
Genetics25197484Genome sequence of the mud-dwelling archaeon Methanoplanus limicola type strain (DSM 2279(T)), reclassification of Methanoplanus petrolearius as Methanolacinia petrolearia and emended descriptions of the genera Methanoplanus and Methanolacinia.Goker M, Lu M, Fiebig A, Nolan M, Lapidus A, Tice H, Del Rio TG, Cheng JF, Han C, Tapia R, Goodwin LA, Pitluck S, Liolios K, Mavromatis K, Pagani I, Ivanova N, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Mayilraj S, Rohde M, Detter JC, Bunk B, Spring S, Wirth R, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HPStand Genomic Sci10.4056/sigs.51389682014Phylogeny
Phylogeny27553654Methanomicrobium antiquum sp. nov., a hydrogenotrophic methanogen isolated from deep sedimentary aquifers in a natural gas field.Mochimaru H, Tamaki H, Katayama T, Imachi H, Sakata S, Kamagata YInt J Syst Evol Microbiol10.1099/ijsem.0.0014442016Base Composition, DNA, Archaeal/genetics, Groundwater/*microbiology, Japan, Methane, Methanomicrobiaceae/*classification/genetics/isolation & purification, *Natural Gas, Oil and Gas Fields/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4325Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11571)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11571
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76502Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49139.1StrainInfo: A central database for resolving microbial strain identifiers