Strain identifier

BacDive ID: 7041

Type strain: Yes

Species: Methanoplanus endosymbiosus

Strain Designation: MC1

Strain history: <- G.D. Vogels, MC1

NCBI tax ID(s): 33865 (species)

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General

@ref: 1421

BacDive-ID: 7041

DSM-Number: 3599

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanoplanus endosymbiosus MC1 is an anaerobe, mesophilic archaeon that was isolated from from marine ciliate Metopus contortus.

NCBI tax id

  • NCBI tax id: 33865
  • Matching level: species

strain history

  • @ref: 1421
  • history: <- G.D. Vogels, MC1

doi: 10.13145/bacdive7041.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanomicrobiales
  • family: Methanomicrobiaceae
  • genus: Methanoplanus
  • species: Methanoplanus endosymbiosus
  • full scientific name: Methanoplanus endosymbiosus van Bruggen et al. 1986

@ref: 1421

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanomicrobiaceae

genus: Methanoplanus

species: Methanoplanus endosymbiosus

full scientific name: Methanoplanus endosymbiosus van Bruggen et al. 1986

strain designation: MC1

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 1421
  • name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/141
  • composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

  • @ref: 1421
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1421
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1421
  • sample type: from marine ciliate Metopus contortus
  • host species: Metopus contortus
  • geographic location: Country of origin unknown, Wadden Sea

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Host#Protozoa

taxonmaps

  • @ref: 69479
  • File name: preview.99_187309.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18124;96_68922;97_89575;98_121867;99_187309&stattab=map
  • Last taxonomy: Methanoplanus
  • 16S sequence: FR733674
  • Sequence Identity:
  • Total samples: 189
  • soil counts: 31
  • aquatic counts: 153
  • animal counts: 1
  • plant counts: 4

Safety information

risk assessment

  • @ref: 1421
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Methanoplanus endosymbiosus gene for 16S ribosomal RNA, partial sequence, strain: DSM 3599AB3702481399ena33865
20218M.endosymbiosus (DSM 3599) mRNA for 16S ribosomal RNAZ29435949ena33865
1421Methanoplanus endosymbiosus partial 16S rRNA gene, type strain DSM3599TFR7336741464ena33865

Genome sequences

  • @ref: 66792
  • description: Methanoplanus endosymbiosus DSM 3599
  • accession: GCA_024662215
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 33865

GC content

  • @ref: 1421
  • GC-content: 38.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno96.976no
gram-positiveno96.767no
anaerobicyes99.282yes
aerobicno97.233yes
halophileno84.743no
spore-formingno93.805no
glucose-fermentno57.146no
thermophileno93.97yes
glucose-utilyes74.506no
motileno91.335no

External links

@ref: 1421

culture collection no.: DSM 3599, OCM 102

straininfo link

  • @ref: 76500
  • straininfo: 47732

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1421Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3599)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3599
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76500Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47732.1StrainInfo: A central database for resolving microbial strain identifiers