General

@ref: 7750

BacDive-ID: 7031

DSM-Number: 18860

keywords: 16S sequence, Archaea, anaerobe, thermophilic

description: Methanoculleus receptaculi ZC-2 is an anaerobe, thermophilic archaeon that was isolated from oil-production water.

NCBI tax id

• NCBI tax id: 394967
• Matching level: species

strain history

• @ref: 7750
• history: <- L. Cheng and T. Qiu; ZC-2

doi: 10.13145/bacdive7031.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanomicrobiales
• family: Methanomicrobiaceae
• genus: Methanoculleus
• species: Methanoculleus receptaculi
• full scientific name: Methanoculleus receptaculi Cheng et al. 2008

@ref: 7750

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanomicrobiaceae

genus: Methanoculleus

species: Methanoculleus receptaculi

full scientific name: Methanoculleus receptaculi Cheng et al. 2008

strain designation: ZC-2

type strain: yes

Culture and growth conditions

culture medium

• @ref: 7750
• name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141
• composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

• @ref: 7750
• growth: positive
• type: growth
• temperature: 50
• range: thermophilic

Physiology and metabolism

oxygen tolerance

• @ref: 7750
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 7750
• sample type: oil-production water
• geographic location: Shengli oil field
• country: China
• origin.country: CHN
• continent: Asia

isolation source categories

Safety information

risk assessment

• @ref: 7750
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 7750
• description: Methanoculleus receptaculi strain ZC-2 16S ribosomal RNA gene, partial sequence
• accession: DQ787476
• length: 1340
• database: ena
• NCBI tax ID: 394967

GC content

• @ref: 7750
• GC-content: 55.2
• method: thermal denaturation, midpoint method (Tm)

External links

@ref: 7750

culture collection no.: DSM 18860, CGMCC 1.5087

straininfo link

• @ref: 76491
• straininfo: 402078

literature

• topic: Phylogeny
• Pubmed-ID: 18557787
• title: Isolation and characterization of Methanoculleus receptaculi sp. nov. from Shengli oil field, China.
• authors: Cheng L, Qiu TL, Li X, Wang WD, Deng Y, Yin XB, Zhang H
• journal: FEMS Microbiol Lett
• DOI: 10.1111/j.1574-6968.2008.01212.x
• year: 2008
• mesh: China, DNA, Bacterial/chemistry/genetics, Methanomicrobiaceae/*chemistry/classification/*isolation & purification/physiology, Molecular Sequence Data, Petroleum/*microbiology, Phylogeny, *Water Microbiology
• topic2: Genetics

Reference