General

@ref: 1712

BacDive-ID: 7021

DSM-Number: 4552

keywords: Archaea, anaerobe, mesophilic

description: Methanoculleus marisnigri AN8 is an anaerobe, mesophilic archaeon that was isolated from pig-manure digestor.

NCBI tax id

• NCBI tax id: 2198
• Matching level: species

strain history

• @ref: 1712
• history: <- D. R. Boone, Oregon Graduate Center, Department of Environmental Science & Engineering, Beaverton, Oregon, USA; OCM 51 <- G. M. Maestrojuan; AN8

doi: 10.13145/bacdive7021.20241212.9.2

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanomicrobiales
• family: Methanomicrobiaceae
• genus: Methanoculleus
• species: Methanoculleus marisnigri
• full scientific name: Methanoculleus marisnigri (Romesser et al. 1981) Maestrojuán et al. 1990

• synonyms

  • @ref: 20215
  • synonym: Methanogenium marisnigri

@ref: 1712

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanomicrobiaceae

genus: Methanoculleus

species: Methanoculleus marisnigri

full scientific name: Methanoculleus marisnigri (Romesser et al. 1981) Maestrojuán et al. 1990

strain designation: AN8

type strain: no

Morphology

colony morphology

• @ref: 1712
• incubation period: 3-7 days

Culture and growth conditions

culture medium

• @ref: 1712
• name: METHANOCULLEUS SP. MEDIUM (DSMZ Medium 141b)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141b
• composition: Name: METHANOCULLEUS SP. MEDIUM (DSMZ Medium 141b) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

• @ref: 1712
• growth: positive
• type: growth
• temperature: 37

Physiology and metabolism

oxygen tolerance

• @ref: 1712
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 1712
• sample type: pig-manure digestor
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

Safety information

risk assessment

• @ref: 1712
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

GC content

• @ref: 1712
• GC-content: 62.0

External links

@ref: 1712

culture collection no.: DSM 4552, OCM 51

straininfo link

• @ref: 76481
• straininfo: 47853

Reference