Strain identifier
BacDive ID: 7020
Type strain:
Species: Methanoculleus marisnigri
Strain Designation: JR 1, JR1
Strain history: <- W. E. Balch, University of Illinois, Urbana, USA; JR 1 <- J. A. Romesser and R. S. Wolfe
NCBI tax ID(s): 2198 (species)
General
@ref: 795
BacDive-ID: 7020
DSM-Number: 1498
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanoculleus marisnigri JR 1 is an anaerobe, mesophilic archaeon that was isolated from marine sediment.
NCBI tax id
- NCBI tax id: 2198
- Matching level: species
strain history
- @ref: 795
- history: <- W. E. Balch, University of Illinois, Urbana, USA; JR 1 <- J. A. Romesser and R. S. Wolfe
doi: 10.13145/bacdive7020.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanomicrobiales
- family: Methanomicrobiaceae
- genus: Methanoculleus
- species: Methanoculleus marisnigri
- full scientific name: Methanoculleus marisnigri (Romesser et al. 1981) Maestrojuán et al. 1990
synonyms
- @ref: 20215
- synonym: Methanogenium marisnigri
@ref: 795
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanomicrobiales
family: Methanomicrobiaceae
genus: Methanoculleus
species: Methanoculleus marisnigri
full scientific name: Methanoculleus marisnigri (Romesser et al. 1981) Maestrojuán et al. 1990
strain designation: JR 1, JR1
type strain: yes
Culture and growth conditions
culture medium
- @ref: 795
- name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141
- composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
- @ref: 795
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 795
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 795
- sample type: marine sediment
- geographic location: Country of origin unknown, Black Sea
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Environmental | #Aquatic | #Sediment |
Safety information
risk assessment
- @ref: 795
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Enterococcus faecalis clone OCM056 16S ribosomal RNA gene, partial sequence | GU417239 | 456 | ena | 1351 |
20218 | Methanoculleus marisnigri strain DSM 1498 16S ribosomal RNA gene, partial sequence | M59134 | 1466 | ena | 2198 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Methanoculleus marisnigri JR1 | GCA_000015825 | complete | ncbi | 368407 |
66792 | Methanoculleus marisnigri JR1, DSM 1498 | 640069318 | complete | img | 368407 |
66792 | Methanoculleus marisnigri JR1 | 368407.15 | complete | patric | 368407 |
GC content
- @ref: 795
- GC-content: 61.2
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 90.488 | no |
flagellated | no | 97.48 | no |
gram-positive | no | 97.037 | no |
anaerobic | yes | 98.6 | no |
halophile | no | 85.244 | no |
spore-forming | no | 95.364 | no |
thermophile | no | 88.258 | yes |
glucose-util | yes | 76.273 | no |
aerobic | no | 96.376 | yes |
glucose-ferment | no | 63.315 | no |
External links
@ref: 795
culture collection no.: DSM 1498, ATCC 35101, OCM 56
straininfo link
- @ref: 76480
- straininfo: 40871
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
795 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1498) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1498 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
76480 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40871.1 | StrainInfo: A central database for resolving microbial strain identifiers |