Strain identifier

BacDive ID: 7016

Type strain: Yes

Species: Methanoculleus bourgensis

Strain Designation: MS2

Strain history: <- B. Ollivier, Université de Provence, Marseille, France; MS2 <- B. Ollivier {1982}

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1284

BacDive-ID: 7016

DSM-Number: 3045

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanoculleus bourgensis MS2 is an anaerobe, mesophilic archaeon that was isolated from sewage sludge digester.

NCBI tax id

NCBI tax idMatching level
1201294strain
83986species

strain history

  • @ref: 1284
  • history: <- B. Ollivier, Université de Provence, Marseille, France; MS2 <- B. Ollivier {1982}

doi: 10.13145/bacdive7016.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanomicrobiales
  • family: Methanomicrobiaceae
  • genus: Methanoculleus
  • species: Methanoculleus bourgensis
  • full scientific name: Methanoculleus bourgensis corrig. (Ollivier et al. 1986) Maestrojuán et al. 1990
  • synonyms

    @refsynonym
    20215Methanogenium olentangyi
    20215Methanoculleus oldenburgensis
    20215Methanoculleus olentangyi
    20215Methanoculleus bourgense
    20215Methanogenium bourgense

@ref: 1284

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanomicrobiaceae

genus: Methanoculleus

species: Methanoculleus bourgensis

full scientific name: Methanoculleus bourgensis (Ollivier et al. 1986) Maestrojuán et al. 1990 emend. Asakawa and Nagaoka 2003

strain designation: MS2

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 1284
  • name: METHANOCULLEUS BOURGENSE MEDIUM (DSMZ Medium 332)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/332
  • composition: Name: METHANOCULLEUS BOURGENSIS MEDIUM (DSMZ Medium 332) Composition: Na-formate 5.0 g/l Na2CO3 1.5 g/l Yeast extract 1.0 g/l Trypticase peptone 1.0 g/l Na-acetate 1.0 g/l NH4Cl 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l K2HPO4 x 3 H2O 0.4 g/l Na2S x 9 H2O 0.2 g/l MgCl2 x 6 H2O 0.1 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

  • @ref: 1284
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1284
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1284
  • sample type: sewage sludge digester
  • geographic location: Bourg en Bresse
  • country: France
  • origin.country: FRA
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation
#Engineered#Waste#Sewage sludge

Safety information

risk assessment

  • @ref: 1284
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Methanoculleus bourgensis 16S small subunit ribosomal RNA gene, partial sequenceAF0952691411ena1201294
20218Enterococcus faecalis clone OCM015 16S ribosomal RNA gene, partial sequenceGU417216460ena1351
1284Methanoculleus bourgensis 16S ribosomal RNA gene, partial sequenceAY1966741255ena1201294

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanoculleus bourgensis MS22540341105completeimg1201294
66792Methanoculleus bourgensis MS21201294.9completepatric1201294

GC content

  • @ref: 1284
  • GC-content: 60.6
  • method: sequence analysis

External links

@ref: 1284

culture collection no.: DSM 3045, ATCC 43281, OCM 15

straininfo link

  • @ref: 76476
  • straininfo: 45124

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1284Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3045)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3045
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
76476Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45124.1StrainInfo: A central database for resolving microbial strain identifiers