Strain identifier
BacDive ID: 7016
Type strain:
Species: Methanoculleus bourgensis
Strain Designation: MS2
Strain history: <- B. Ollivier, Université de Provence, Marseille, France; MS2 <- B. Ollivier {1982}
NCBI tax ID(s): 1201294 (strain), 83986 (species)
General
@ref: 1284
BacDive-ID: 7016
DSM-Number: 3045
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanoculleus bourgensis MS2 is an anaerobe, mesophilic archaeon that was isolated from sewage sludge digester.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1201294 | strain |
83986 | species |
strain history
- @ref: 1284
- history: <- B. Ollivier, Université de Provence, Marseille, France; MS2 <- B. Ollivier {1982}
doi: 10.13145/bacdive7016.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanomicrobiales
- family: Methanomicrobiaceae
- genus: Methanoculleus
- species: Methanoculleus bourgensis
- full scientific name: Methanoculleus bourgensis corrig. (Ollivier et al. 1986) Maestrojuán et al. 1990
synonyms
@ref synonym 20215 Methanogenium olentangyi 20215 Methanoculleus oldenburgensis 20215 Methanoculleus olentangyi 20215 Methanoculleus bourgense 20215 Methanogenium bourgense
@ref: 1284
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanomicrobiales
family: Methanomicrobiaceae
genus: Methanoculleus
species: Methanoculleus bourgensis
full scientific name: Methanoculleus bourgensis (Ollivier et al. 1986) Maestrojuán et al. 1990 emend. Asakawa and Nagaoka 2003
strain designation: MS2
type strain: yes
Culture and growth conditions
culture medium
- @ref: 1284
- name: METHANOCULLEUS BOURGENSE MEDIUM (DSMZ Medium 332)
- growth: yes
- link: https://mediadive.dsmz.de/medium/332
- composition: Name: METHANOCULLEUS BOURGENSIS MEDIUM (DSMZ Medium 332) Composition: Na-formate 5.0 g/l Na2CO3 1.5 g/l Yeast extract 1.0 g/l Trypticase peptone 1.0 g/l Na-acetate 1.0 g/l NH4Cl 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l K2HPO4 x 3 H2O 0.4 g/l Na2S x 9 H2O 0.2 g/l MgCl2 x 6 H2O 0.1 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
- @ref: 1284
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1284
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 1284
- sample type: sewage sludge digester
- geographic location: Bourg en Bresse
- country: France
- origin.country: FRA
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Biodegradation | |
#Engineered | #Waste | #Sewage sludge |
Safety information
risk assessment
- @ref: 1284
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Methanoculleus bourgensis 16S small subunit ribosomal RNA gene, partial sequence | AF095269 | 1411 | ena | 1201294 |
20218 | Enterococcus faecalis clone OCM015 16S ribosomal RNA gene, partial sequence | GU417216 | 460 | ena | 1351 |
1284 | Methanoculleus bourgensis 16S ribosomal RNA gene, partial sequence | AY196674 | 1255 | ena | 1201294 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Methanoculleus bourgensis MS2 | 2540341105 | complete | img | 1201294 |
66792 | Methanoculleus bourgensis MS2 | 1201294.9 | complete | patric | 1201294 |
GC content
- @ref: 1284
- GC-content: 60.6
- method: sequence analysis
External links
@ref: 1284
culture collection no.: DSM 3045, ATCC 43281, OCM 15
straininfo link
- @ref: 76476
- straininfo: 45124
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1284 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3045) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3045 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
76476 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45124.1 | StrainInfo: A central database for resolving microbial strain identifiers |