General

@ref: 719

BacDive-ID: 7002

DSM-Number: 2095

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic

description: Methanothermococcus thermolithotrophicus SN-1 is an anaerobe, thermophilic archaeon that was isolated from geothermally heated sea floor.

NCBI tax id

strain history

doi: 10.13145/bacdive7002.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanococci
• order: Methanococcales
• family: Methanococcaceae
• genus: Methanothermococcus
• species: Methanothermococcus thermolithotrophicus
• full scientific name: Methanothermococcus thermolithotrophicus (Huber et al. 1984) Whitman 2002

• synonyms

  • @ref: 20215
  • synonym: Methanococcus thermolithotrophicus

@ref: 719

domain: Archaea

phylum: Euryarchaeota

class: Methanococci

order: Methanococcales

family: Methanococcaceae

genus: Methanothermococcus

species: Methanothermococcus thermolithotrophicus

full scientific name: Methanothermococcus thermolithotrophicus (Huber et al. 1984) Whitman 2002

strain designation: SN-1

type strain: yes

Culture and growth conditions

culture medium

• @ref: 719
• name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141
• composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

Physiology and metabolism

oxygen tolerance

• @ref: 719
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

isolation source categories

Safety information

risk assessment

• @ref: 719
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 719
• description: Methanothermococcus thermolithotrophicus DSM 2095 16S ribosomal RNA gene, partial sequence
• accession: M59128
• length: 1452
• database: ena
• NCBI tax ID: 523845

Genome sequences

Genome-based predictions

predictions

External links

@ref: 719

culture collection no.: DSM 2095, ATCC 35097, JCM 10549, OCM 138

straininfo link

• @ref: 76463
• straininfo: 44996

literature

• Pubmed-ID: 36409126
• title: Comparative Transcriptomics Sheds Light on Remodeling of Gene Expression during Diazotrophy in the Thermophilic Methanogen Methanothermococcus thermolithotrophicus.
• authors: Maslac N, Sidhu C, Teeling H, Wagner T
• journal: mBio
• DOI: 10.1128/mbio.02443-22
• year: 2022

Reference