Strain identifier

BacDive ID: 7000

Type strain: No

Species: Methanococcus aeolicus

Strain Designation: PL-15/H

Strain history: <- D. Boone and M. Kendall, Portland State Univ., USA; PL-15/H <- K. Stetter {1980}

NCBI tax ID(s): 42879 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6853

BacDive-ID: 7000

DSM-Number: 17251

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanococcus aeolicus PL-15/H is an anaerobe, mesophilic archaeon that was isolated from marine sediment.

NCBI tax id

  • NCBI tax id: 42879
  • Matching level: species

strain history

  • @ref: 6853
  • history: <- D. Boone and M. Kendall, Portland State Univ., USA; PL-15/H <- K. Stetter {1980}

doi: 10.13145/bacdive7000.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanococci
  • order: Methanococcales
  • family: Methanococcaceae
  • genus: Methanococcus
  • species: Methanococcus aeolicus
  • full scientific name: Methanococcus aeolicus Kendall et al. 2006

@ref: 6853

domain: Archaea

phylum: Euryarchaeota

class: Methanococci

order: Methanococcales

family: Methanococcaceae

genus: Methanococcus

species: Methanococcus aeolicus

full scientific name: Methanococcus aeolicus Kendall et al. 2006

strain designation: PL-15/H

type strain: no

Culture and growth conditions

culture medium

  • @ref: 6853
  • name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/141
  • composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

  • @ref: 6853
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 6853
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 6853
  • sample type: marine sediment
  • geographic location: Lipari Islands near Sicily
  • country: Italy
  • origin.country: ITA
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_190190.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17939;96_70207;97_91176;98_123904;99_190190&stattab=map
  • Last taxonomy: Methanococcus
  • 16S sequence: DQ136171
  • Sequence Identity:
  • Total samples: 15
  • aquatic counts: 8
  • animal counts: 7

Safety information

risk assessment

  • @ref: 6853
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6853
  • description: Methanococcus aeolicus strain PL-15/HP 16S ribosomal RNA gene, partial sequence
  • accession: DQ136171
  • length: 1393
  • database: ena
  • NCBI tax ID: 42879

External links

@ref: 6853

culture collection no.: DSM 17251, OCM 836

straininfo link

  • @ref: 76461
  • straininfo: 399706

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology6324124Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus.Schmid K, Thomm M, Laminet A, Laue FG, Kessler C, Stetter KO, Schmitt RNucleic Acids Res10.1093/nar/12.6.26191984Base Sequence, DNA Restriction Enzymes/*isolation & purification/metabolism, *Deoxyribonucleases, Type II Site-Specific, Euryarchaeota/*enzymology, Kinetics, Substrate SpecificityPhylogeny
Phylogeny16825624Methanococcus aeolicus sp. nov., a mesophilic, methanogenic archaeon from shallow and deep marine sediments.Kendall MM, Liu Y, Sieprawska-Lupa M, Stetter KO, Whitman WB, Boone DRInt J Syst Evol Microbiol10.1099/ijs.0.64216-02006Archaeal Proteins/analysis, Base Composition, DNA, Archaeal/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Genes, rRNA, Geologic Sediments/*microbiology, Mediterranean Sea, Methanococcus/chemistry/*classification/*isolation & purification/physiology, Molecular Sequence Data, Pacific Ocean, Phylogeny, Proteome/analysis, RNA, Archaeal/genetics, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Temperature, Water MicrobiologyEnzymology

Reference

@idauthorscataloguedoi/urltitle
6853Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17251)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17251
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76461Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID399706.1StrainInfo: A central database for resolving microbial strain identifiers