Strain identifier
BacDive ID: 6991
Type strain: 
Species: Methanococcus maripaludis
Strain Designation: LL, S2
Strain history: DSM 14266 <-- J. A. Leigh <-- W. B. Witman S2.
NCBI tax ID(s): 39152 (species)
version 9.2 (current version)
General
@ref: 5291
BacDive-ID: 6991
DSM-Number: 14266
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanococcus maripaludis LL is an anaerobe, mesophilic archaeon that was isolated from salt marsh and intertidal sediments.
NCBI tax id
- NCBI tax id: 39152
- Matching level: species
strain history
| @ref | history |
|---|---|
| 5291 | <- J. A. Leigh, University of Washington, Seattle, Washington, USA; LL <- W. B. Whitman {1986} |
| 67770 | DSM 14266 <-- J. A. Leigh <-- W. B. Witman S2. |
doi: 10.13145/bacdive6991.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanococci
- order: Methanococcales
- family: Methanococcaceae
- genus: Methanococcus
- species: Methanococcus maripaludis
- full scientific name: Methanococcus maripaludis Jones et al. 1984
synonyms
- @ref: 20215
- synonym: Methanococcus deltae
@ref: 5291
domain: Archaea
phylum: Euryarchaeota
class: Methanococci
order: Methanococcales
family: Methanococcaceae
genus: Methanococcus
species: Methanococcus maripaludis
full scientific name: Methanococcus maripaludis Jones et al. 1984
strain designation: LL, S2
type strain: no
Culture and growth conditions
culture medium
- @ref: 5291
- name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141
- composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 5291 | positive | growth | 37 |
| 67770 | positive | growth | 37 |
Physiology and metabolism
oxygen tolerance
- @ref: 5291
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 5291 | salt marsh and intertidal sediments | Southeastern United States | USA | USA | North America |
| 67770 | Sediment on the bank of the Latham River | near Jekyll Island, GA | USA | USA | North America |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Salt marsh |
| #Environmental | #Terrestrial | #Tidal flat |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_191917.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17939;96_69441;97_90195;98_122596;99_191917&stattab=map
- Last taxonomy: Methanococcus
- 16S sequence: AF005049
- Sequence Identity:
- Total samples: 220
- soil counts: 9
- aquatic counts: 93
- animal counts: 118
Safety information
risk assessment
- @ref: 5291
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5291
- description: Methanococcus maripaludis 16S ribosomal RNA gene, complete sequence
- accession: AF005049
- length: 1392
- database: nuccore
- NCBI tax ID: 39152
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanococcus maripaludis S2 | GCA_000011585 | complete | ncbi | 267377 |
| 66792 | Methanococcus maripaludis S2 | 2563366543 | complete | img | 267377 |
| 66792 | Methanococcus maripaludis S2 | 267377.15 | complete | patric | 267377 |
GC content
- @ref: 67770
- GC-content: 34.4
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 65.371 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 88.818 | yes |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 88.474 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 88.571 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 71.963 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 83.5 | no |
External links
@ref: 5291
culture collection no.: DSM 14266, JCM 13030, NBRC 101832
straininfo link
- @ref: 76452
- straininfo: 49551
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5291 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14266) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14266 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76452 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49551.1 | StrainInfo: A central database for resolving microbial strain identifiers |