Strain identifier
BacDive ID: 6988
Type strain: 
Species: Methanotorris formicicus
Strain Designation: Mc-S-70
Strain history: K. Takai Mc-S-70.
NCBI tax ID(s): 647171 (strain), 213185 (species)
version 8.1 (current version)
General
@ref: 6590
BacDive-ID: 6988
DSM-Number: 16983
keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic
description: Methanotorris formicicus Mc-S-70 is an anaerobe, thermophilic archaeon that was isolated from black smoker vent, depth of 2421 m.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 647171 | strain |
| 213185 | species |
strain history
| @ref | history |
|---|---|
| 6590 | <- JCM <- K. Takai, Subground Animalcule Retrieval (SUGAR) Program, Japan Agency for Marine-Earth Science and Technology (JAMSTEC); Mc-S-70 |
| 67770 | K. Takai Mc-S-70. |
doi: 10.13145/bacdive6988.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanococci
- order: Methanococcales
- family: Methanocaldococcaceae
- genus: Methanotorris
- species: Methanotorris formicicus
- full scientific name: Methanotorris formicicus Takai et al. 2004
@ref: 6590
domain: Archaea
phylum: Euryarchaeota
class: Methanococci
order: Methanococcales
family: Methanocaldococcaceae
genus: Methanotorris
species: Methanotorris formicicus
full scientific name: Methanotorris formicicus Takai et al. 2004
strain designation: Mc-S-70
type strain: yes
Culture and growth conditions
culture medium
- @ref: 6590
- name: METHANOCALDOCOCCUS MEDIUM (DSMZ Medium 282)
- growth: yes
- link: https://mediadive.dsmz.de/medium/282
- composition: Name: METHANOCALDOCOCCUS MEDIUM (DSMZ Medium 282) Composition: MgCl2 x 6 H2O 4.05138 g/l NaHCO3 0.988142 g/l Na2S x 9 H2O 0.494071 g/l L-Cysteine HCl x H2O 0.494071 g/l KCl 0.326087 g/l NH4Cl 0.247036 g/l K2HPO4 0.13834 g/l MgSO4 x 7 H2O 0.0296443 g/l Nitrilotriacetic acid 0.0148221 g/l NaCl 0.00988142 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00988142 g/l MnSO4 x H2O 0.00494071 g/l ZnSO4 x 7 H2O 0.00177866 g/l CoSO4 x 7 H2O 0.00177866 g/l CaCl2 x 2 H2O 0.000988142 g/l FeSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l NiCl2 x 6 H2O 0.000296443 g/l AlK(SO4)2 x 12 H2O 0.000197628 g/l Na2MoO4 x 2 H2O 9.88142e-05 g/l CuSO4 x 5 H2O 9.88142e-05 g/l H3BO3 9.88142e-05 g/l Pyridoxine hydrochloride 9.88142e-05 g/l Thiamine HCl 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l p-Aminobenzoic acid 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l Nicotinic acid 4.94071e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Biotin 1.97628e-05 g/l Folic acid 1.97628e-05 g/l Na2WO4 x 2 H2O 3.95257e-06 g/l Na2SeO3 x 5 H2O 2.96443e-06 g/l Vitamin B12 9.88142e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6590 | positive | growth | 70 | thermophilic |
| 67770 | positive | growth | 70 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 6590
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | latitude | longitude |
|---|---|---|---|---|---|
| 6590 | black smoker vent, depth of 2421 m | Central Indian Ridge, Kairei hydrothermal field | International waters | -25.3231 | 70.045 |
| 67770 | Black smoker chimney at the Kairei field in the Central Indian Ridge |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_188708.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17708;96_69608;97_90405;98_122878;99_188708&stattab=map
- Last taxonomy: Methanocaldococcaceae
- 16S sequence: AB100884
- Sequence Identity:
- Total samples: 1
- aquatic counts: 1
Safety information
risk assessment
- @ref: 6590
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6590
- description: Methanotorris formicicus rrn gene for small subunit ribosomal RNA, partial sequence
- accession: AB100884
- length: 1374
- database: ena
- NCBI tax ID: 647171
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanotorris formicicus Mc-S-70 | 2506520039 | draft | img | 647171 |
| 67770 | Methanotorris formicicus Mc-S-70 | GCA_000243455 | contig | ncbi | 647171 |
| 66792 | Methanotorris formicicus Mc-S-70 | 647171.129 | wgs | patric | 647171 |
GC content
- @ref: 6590
- GC-content: 33.3
- method: high performance liquid chromatography (HPLC)
External links
@ref: 6590
culture collection no.: DSM 16983, ATCC BAA 687, JCM 11930
straininfo link
- @ref: 76449
- straininfo: 132786
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 15280275 | Methanotorris formicicus sp. nov., a novel extremely thermophilic, methane-producing archaeon isolated from a black smoker chimney in the Central Indian Ridge. | Takai K, Nealson KH, Horikoshi K | Int J Syst Evol Microbiol | 10.1099/ijs.0.02887-0 | 2004 | Anaerobiosis, Base Composition, Carbon Dioxide/metabolism, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry, Flagella/ultrastructure, Formates/metabolism, Genes, rRNA, Hydrogen/metabolism, Hydrogen-Ion Concentration, Indian Ocean, Methane/*metabolism, Methanococcales/*classification/cytology/growth & development/*isolation & purification, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Temperature, Water Microbiology | Metabolism |
| Phylogeny | 30843780 | Methanofervidicoccus abyssi gen. nov., sp. nov., a hydrogenotrophic methanogen, isolated from a hydrothermal vent chimney in the Mid-Cayman Spreading Center, the Caribbean Sea. | Sakai S, Takaki Y, Miyazaki M, Ogawara M, Yanagawa K, Miyazaki J, Takai K | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003297 | 2019 | Caribbean Region, DNA, Archaeal/genetics, Genes, Archaeal, Hydrothermal Vents/*microbiology, Methanococcaceae/*classification/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater, Sequence Analysis, DNA, Temperature | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 6590 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16983) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16983 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 76449 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID132786.1 | StrainInfo: A central database for resolving microbial strain identifiers |