Archive BacDiveID:10.13145/bacdive6985.20230509.8.1

Strain identifier

BacDive ID: 6985

Type strain:

Species: Methanocaldococcus indicus

Strain Designation: SL43

Strain history: S. L'Haridon and C. Jeanthon SL43.

NCBI tax ID(s): 213231 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 5678

BacDive-ID: 6985

DSM-Number: 15027

keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Methanocaldococcus indicus SL43 is an anaerobe, hyperthermophilic archaeon that was isolated from deep-sea hydrothermal chimney sample, 2420m depth.

NCBI tax id

NCBI tax id: 213231
Matching level: species

strain history

@ref	history
5678	<- S. L'Haridon, Centre National de la Recherche Scientifique, and Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Plouzané, France; SL43 <- S. L'Haridon {2001}
67770	S. L'Haridon and C. Jeanthon SL43.

doi: 10.13145/bacdive6985.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/methanobacteriota
domain: Archaea
phylum: Methanobacteriota
class: Methanococci
order: Methanococcales
family: Methanocaldococcaceae
genus: Methanocaldococcus
species: Methanocaldococcus indicus
full scientific name: Methanocaldococcus indicus L'Haridon et al. 2003

@ref: 5678

domain: Archaea

phylum: Euryarchaeota

class: Methanococci

order: Methanococcales

family: Methanocaldococcaceae

genus: Methanocaldococcus

species: Methanocaldococcus indicus

full scientific name: Methanocaldococcus indicus L'Haridon et al. 2003

strain designation: SL43

type strain: yes

Culture and growth conditions

culture medium

@ref: 5678
name: METHANOCALDOCOCCUS MEDIUM (DSMZ Medium 282)
growth: yes
link: https://mediadive.dsmz.de/medium/282
composition: Name: METHANOCALDOCOCCUS MEDIUM (DSMZ Medium 282) Composition: MgCl2 x 6 H2O 4.05138 g/l NaHCO3 0.988142 g/l Na2S x 9 H2O 0.494071 g/l L-Cysteine HCl x H2O 0.494071 g/l KCl 0.326087 g/l NH4Cl 0.247036 g/l K2HPO4 0.13834 g/l MgSO4 x 7 H2O 0.0296443 g/l Nitrilotriacetic acid 0.0148221 g/l NaCl 0.00988142 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00988142 g/l MnSO4 x H2O 0.00494071 g/l ZnSO4 x 7 H2O 0.00177866 g/l CoSO4 x 7 H2O 0.00177866 g/l CaCl2 x 2 H2O 0.000988142 g/l FeSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l NiCl2 x 6 H2O 0.000296443 g/l AlK(SO4)2 x 12 H2O 0.000197628 g/l Na2MoO4 x 2 H2O 9.88142e-05 g/l CuSO4 x 5 H2O 9.88142e-05 g/l H3BO3 9.88142e-05 g/l Pyridoxine hydrochloride 9.88142e-05 g/l Thiamine HCl 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l p-Aminobenzoic acid 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l Nicotinic acid 4.94071e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Biotin 1.97628e-05 g/l Folic acid 1.97628e-05 g/l Na2WO4 x 2 H2O 3.95257e-06 g/l Na2SeO3 x 5 H2O 2.96443e-06 g/l Vitamin B12 9.88142e-07 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
5678	positive	growth	85	hyperthermophilic
67770	positive	growth	80	hyperthermophilic

Physiology and metabolism

oxygen tolerance

@ref: 5678
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	latitude	longitude
5678	deep-sea hydrothermal chimney sample, 2420m depth	Central Indian Ridge, Kairei vent field	International waters	-25.3167	70.0333
67770	Deep-sea hydrothermal chimney on the Central Indian Ridge at a depth of 2,420 m

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Marine
#Condition	#Thermophilic (>45°C)

taxonmaps

@ref: 69479
File name: preview.99_190992.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17891;96_70513;97_91572;98_124449;99_190992&stattab=map
Last taxonomy: Methanocaldococcus indicus subclade
16S sequence: AF547621
Sequence Identity:
Total samples: 3
aquatic counts: 3

Safety information

risk assessment

@ref: 5678
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 5678
description: Methanocaldococcus indiensis 16S ribosomal RNA gene, partial sequence
accession: AF547621
length: 1409
database: ena
NCBI tax ID: 213231

GC content

@ref: 5678
GC-content: 30.7
method: high performance liquid chromatography (HPLC)

External links

@ref: 5678

culture collection no.: DSM 15027, JCM 11886

straininfo link

@ref: 76446
straininfo: 127999

literature

topic: Phylogeny
Pubmed-ID: 14657125
title: Methanocaldococcus indicus sp. nov., a novel hyperthermophilic methanogen isolated from the Central Indian Ridge.
authors: L'Haridon S, Reysenbach AL, Banta A, Messner P, Schumann P, Stackebrandt E, Jeanthon C
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.02700-0
year: 2003
mesh: Flagella/ultrastructure, Methanococcus/*classification/*genetics/isolation & purification/ultrastructure, Microscopy, Electron, Molecular Sequence Data, *Phylogeny, Seawater/microbiology, Water Microbiology
topic2: Genetics

Reference

@id	authors	catalogue	doi/url	title
5678	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15027)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-15027
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
76446	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID127999.1	StrainInfo: A central database for resolving microbial strain identifiers