Strain identifier
BacDive ID: 6983
Type strain: 
Species: Methanocaldococcus vulcanius
Strain Designation: M7
Strain history: <- C. Jeanthon, M7
NCBI tax ID(s): 73913 (species)
version 8.1 (current version)
General
@ref: 4602
BacDive-ID: 6983
DSM-Number: 12094
keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Methanocaldococcus vulcanius M7 is an anaerobe, hyperthermophilic archaeon that was isolated from hydrothermal vent chimney, 2600 m depth.
NCBI tax id
- NCBI tax id: 73913
- Matching level: species
strain history
- @ref: 4602
- history: <- C. Jeanthon, M7
doi: 10.13145/bacdive6983.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanococci
- order: Methanococcales
- family: Methanocaldococcaceae
- genus: Methanocaldococcus
- species: Methanocaldococcus vulcanius
- full scientific name: Methanocaldococcus vulcanius (Jeanthon et al. 1999) Whitman 2002
synonyms
- @ref: 20215
- synonym: Methanococcus vulcanius
@ref: 4602
domain: Archaea
phylum: Euryarchaeota
class: Methanococci
order: Methanococcales
family: Methanocaldococcaceae
genus: Methanocaldococcus
species: Methanocaldococcus vulcanius
full scientific name: Methanocaldococcus vulcanius (Jeanthon et al. 1999) Whitman 2002
strain designation: M7
type strain: yes
Culture and growth conditions
culture medium
- @ref: 4602
- name: METHANOCALDOCOCCUS MEDIUM (DSMZ Medium 282)
- growth: yes
- link: https://mediadive.dsmz.de/medium/282
- composition: Name: METHANOCALDOCOCCUS MEDIUM (DSMZ Medium 282) Composition: MgCl2 x 6 H2O 4.05138 g/l NaHCO3 0.988142 g/l Na2S x 9 H2O 0.494071 g/l L-Cysteine HCl x H2O 0.494071 g/l KCl 0.326087 g/l NH4Cl 0.247036 g/l K2HPO4 0.13834 g/l MgSO4 x 7 H2O 0.0296443 g/l Nitrilotriacetic acid 0.0148221 g/l NaCl 0.00988142 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00988142 g/l MnSO4 x H2O 0.00494071 g/l ZnSO4 x 7 H2O 0.00177866 g/l CoSO4 x 7 H2O 0.00177866 g/l CaCl2 x 2 H2O 0.000988142 g/l FeSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l NiCl2 x 6 H2O 0.000296443 g/l AlK(SO4)2 x 12 H2O 0.000197628 g/l Na2MoO4 x 2 H2O 9.88142e-05 g/l CuSO4 x 5 H2O 9.88142e-05 g/l H3BO3 9.88142e-05 g/l Pyridoxine hydrochloride 9.88142e-05 g/l Thiamine HCl 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l p-Aminobenzoic acid 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l Nicotinic acid 4.94071e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Biotin 1.97628e-05 g/l Folic acid 1.97628e-05 g/l Na2WO4 x 2 H2O 3.95257e-06 g/l Na2SeO3 x 5 H2O 2.96443e-06 g/l Vitamin B12 9.88142e-07 g/l Distilled water
culture temp
- @ref: 4602
- growth: positive
- type: growth
- temperature: 80
- range: hyperthermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4602
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 4602
- sample type: hydrothermal vent chimney, 2600 m depth
- geographic location: East Pacific Rise
- latitude: 12.8
- longitude: -103.933
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 4602
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4602
- description: Methanococcus vulcanus 16S ribosomal RNA gene, complete sequence
- accession: AF051404
- length: 1424
- database: ena
- NCBI tax ID: 579137
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanocaldococcus vulcanius M7 | GCA_000024625 | complete | ncbi | 579137 |
| 66792 | Methanocaldococcus vulcanius M7, DSM 12094 | 646311944 | complete | img | 579137 |
| 66792 | Methanocaldococcus vulcanius M7 | 579137.10 | complete | patric | 579137 |
GC content
- @ref: 4602
- GC-content: 31
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 85.021 | no |
| flagellated | no | 94.012 | no |
| gram-positive | no | 96.04 | no |
| anaerobic | yes | 99.235 | yes |
| aerobic | no | 97.159 | yes |
| halophile | no | 87.15 | no |
| spore-forming | no | 96.35 | no |
| glucose-util | yes | 63.624 | no |
| thermophile | yes | 99.502 | yes |
| glucose-ferment | no | 55.171 | no |
External links
@ref: 4602
culture collection no.: DSM 12094
straininfo link
- @ref: 76444
- straininfo: 44176
literature
- topic: Phylogeny
- Pubmed-ID: 10319479
- title: Methanococcus vulcanius sp. nov., a novel hyperthermophilic methanogen isolated from East Pacific Rise, and identification of Methanococcus sp. DSM 4213T as Methanococcus fervens sp. nov.
- authors: Jeanthon C, L'Haridon S, Reysenbach AL, Corre E, Vernet M, Messner P, Sleytr UB, Prieur D
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-49-2-583
- year: 1999
- mesh: Base Composition, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Methanococcus/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Pacific Ocean, Phylogeny, RNA, Ribosomal, 16S/genetics, *Water Microbiology
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4602 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12094) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12094 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76444 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44176.1 | StrainInfo: A central database for resolving microbial strain identifiers |