Strain identifier
BacDive ID: 6979
Type strain: 
Species: Methanosphaera cuniculi
Strain Designation: 1R-7
Strain history: <- B. Biavati, 1R-7
NCBI tax ID(s): 1077256 (species)
version 8.1 (current version)
General
@ref: 1589
BacDive-ID: 6979
DSM-Number: 4103
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanosphaera cuniculi 1R-7 is an anaerobe, mesophilic archaeon that was isolated from rectum of rabbit.
NCBI tax id
- NCBI tax id: 1077256
- Matching level: species
strain history
- @ref: 1589
- history: <- B. Biavati, 1R-7
doi: 10.13145/bacdive6979.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanosphaera
- species: Methanosphaera cuniculi
- full scientific name: Methanosphaera cuniculi Biavati et al. 1990
@ref: 1589
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanosphaera
species: Methanosphaera cuniculi
full scientific name: Methanosphaera cuniculi Biavati et al. 1990
strain designation: 1R-7
type strain: yes
Culture and growth conditions
culture medium
- @ref: 1589
- name: METHANOSPHAERA CUNICULI MEDIUM (DSMZ Medium 404)
- growth: yes
- link: https://mediadive.dsmz.de/medium/404
- composition: Name: METHANOSPHAERA CUNICULI MEDIUM (DSMZ Medium 404) Composition: Methanol 7.88845 g/l Na-acetate x 3 H2O 2.98805 g/l NaHCO3 2.98805 g/l Yeast extract 0.996016 g/l Trypticase peptone 0.996016 g/l L-Cysteine HCl x H2O 0.298805 g/l Na2S x 9 H2O 0.298805 g/l K2HPO4 0.239044 g/l KH2PO4 0.239044 g/l (NH4)2SO4 0.239044 g/l MgSO4 x 7 H2O 0.0298805 g/l Nitrilotriacetic acid 0.0149402 g/l NaCl 0.00996016 g/l MnSO4 x H2O 0.00498008 g/l Fe(NH4)2(SO4)2 x 7 H2O 0.00199203 g/l CoSO4 x 7 H2O 0.00179283 g/l ZnSO4 x 7 H2O 0.00179283 g/l CaCl2 x 2 H2O 0.000996016 g/l FeSO4 x 7 H2O 0.000996016 g/l Sodium resazurin 0.000498008 g/l NiCl2 x 6 H2O 0.000298805 g/l AlK(SO4)2 x 12 H2O 0.000199203 g/l Na2MoO4 x 2 H2O 9.96016e-05 g/l CuSO4 x 5 H2O 9.96016e-05 g/l H3BO3 9.96016e-05 g/l Pyridoxine hydrochloride 9.96016e-05 g/l Riboflavin 4.98008e-05 g/l Nicotinic acid 4.98008e-05 g/l Calcium D-(+)-pantothenate 4.98008e-05 g/l p-Aminobenzoic acid 4.98008e-05 g/l (DL)-alpha-Lipoic acid 4.98008e-05 g/l Thiamine HCl 4.98008e-05 g/l Biotin 1.99203e-05 g/l Folic acid 1.99203e-05 g/l Na2WO4 x 2 H2O 3.98406e-06 g/l Na2SeO3 x 5 H2O 2.98805e-06 g/l Vitamin B12 9.96016e-07 g/l Distilled water
culture temp
- @ref: 1589
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1589
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 1589
- sample type: rectum of rabbit
- geographic location: Bologna
- country: Italy
- origin.country: ITA
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Leporidae (Rabbit/Hare) |
| #Host Body-Site | #Gastrointestinal tract | #Rectum |
Safety information
risk assessment
- @ref: 1589
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 1589
- description: Methanosphaera cuniculi partial 16S rRNA gene, type strain DSM 4103T
- accession: HE582783
- length: 1486
- database: ena
- NCBI tax ID: 1077256
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanosphaera cuniculi 1R-7 | GCA_002287195 | contig | ncbi | 1077256 |
| 66792 | Methanosphaera cuniculi DSM 4103 | GCA_003149675 | contig | ncbi | 1077256 |
| 66792 | Methanosphaera cuniculi 1R-7 | 2802429591 | draft | img | 1077256 |
| 66792 | Methanosphaera cuniculi strain DSM 4103 | 1077256.4 | wgs | patric | 1077256 |
GC content
- @ref: 1589
- GC-content: 23.0
External links
@ref: 1589
culture collection no.: DSM 4103, OCM 183
straininfo link
- @ref: 76440
- straininfo: 47793
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 1589 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4103) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4103 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76440 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47793.1 | StrainInfo: A central database for resolving microbial strain identifiers |