Strain identifier
BacDive ID: 6977
Type strain: 
Species: Methanobrevibacter olleyae
Strain Designation: 1H5-1P
Strain history: <- S. Rea, CSIRO Minerals, Bentley, Australia; 1H5-1P <- K. Munyard
NCBI tax ID(s): 294671 (species)
version 8.1 (current version)
General
@ref: 6536
BacDive-ID: 6977
DSM-Number: 16632
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanobrevibacter olleyae 1H5-1P is an anaerobe, mesophilic archaeon that was isolated from ovine rumen fluid.
NCBI tax id
- NCBI tax id: 294671
- Matching level: species
strain history
- @ref: 6536
- history: <- S. Rea, CSIRO Minerals, Bentley, Australia; 1H5-1P <- K. Munyard
doi: 10.13145/bacdive6977.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanobrevibacter
- species: Methanobrevibacter olleyae
- full scientific name: Methanobrevibacter olleyae Rea et al. 2007
@ref: 6536
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobrevibacter
species: Methanobrevibacter olleyae
full scientific name: Methanobrevibacter olleyae Rea et al. 2007
strain designation: 1H5-1P
type strain: yes
Culture and growth conditions
culture medium
- @ref: 6536
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119; with strain-specific modifications) Composition: NaHCO3 3.98804 g/l Trypticase peptone 1.99402 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l KH2PO4 0.498504 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l NH4Cl 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid H2SO4 DL-2-Methylbutyric acid Isovaleric acid Valeric acid Sludge Distilled water
culture temp
- @ref: 6536
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 6536
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 6536
- sample type: ovine rumen fluid
- geographic location: Perth
- country: Australia
- origin.country: AUS
- continent: Australia and Oceania
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Caprinae (Sheep/Goat) |
| #Host Body Product | #Fluids | #Rumen fluid |
Safety information
risk assessment
- @ref: 6536
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6536
- description: Methanobrevibacter olleyae strain KM1H5-1P 16S ribosomal RNA gene, partial sequence
- accession: AY615201
- length: 1266
- database: ena
- NCBI tax ID: 294671
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanobrevibacter olleyae DSM 16632 | GCA_900114585 | scaffold | ncbi | 294671 |
| 66792 | Methanobrevibacter olleyae DSM 16632 | 2593339150 | draft | img | 294671 |
| 66792 | Methanobrevibacter olleyae strain DSM 16632 | 294671.4 | wgs | patric | 294671 |
GC content
- @ref: 6536
- GC-content: 27-29
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 92.611 | no |
| flagellated | no | 97.202 | no |
| gram-positive | no | 94.461 | no |
| anaerobic | yes | 99.067 | yes |
| aerobic | no | 96.33 | yes |
| halophile | no | 80.149 | no |
| spore-forming | no | 95.619 | no |
| glucose-ferment | no | 53.792 | no |
| thermophile | no | 95.976 | yes |
| glucose-util | yes | 60.454 | no |
External links
@ref: 6536
culture collection no.: DSM 16632, OCM 841
straininfo link
- @ref: 76438
- straininfo: 402392
literature
- topic: Phylogeny
- Pubmed-ID: 17329767
- title: Methanobrevibacter millerae sp. nov. and Methanobrevibacter olleyae sp. nov., methanogens from the ovine and bovine rumen that can utilize formate for growth.
- authors: Rea S, Bowman JP, Popovski S, Pimm C, Wright AG
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63984-0
- year: 2007
- mesh: Animals, Cattle/*microbiology, DNA, Archaeal/genetics, DNA, Ribosomal/genetics, Formates/*metabolism, Methanobrevibacter/*classification/genetics/growth & development/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Rumen/*microbiology, Sheep/*microbiology
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 6536 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16632) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16632 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76438 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID402392.1 | StrainInfo: A central database for resolving microbial strain identifiers |