Strain identifier
BacDive ID: 6974
Type strain: 
Species: Methanobrevibacter thaueri
Strain Designation: CW
Strain history: <- T. L. Miller, New York State Department of Health, Albany, New York, USA; CW <- T. L. Miller {1983}
NCBI tax ID(s): 190975 (species)
version 8.1 (current version)
General
@ref: 4574
BacDive-ID: 6974
DSM-Number: 11995
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanobrevibacter thaueri CW is an anaerobe, mesophilic archaeon that was isolated from bovine faeces.
NCBI tax id
- NCBI tax id: 190975
- Matching level: species
strain history
- @ref: 4574
- history: <- T. L. Miller, New York State Department of Health, Albany, New York, USA; CW <- T. L. Miller {1983}
doi: 10.13145/bacdive6974.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanobrevibacter
- species: Methanobrevibacter thaueri
- full scientific name: Methanobrevibacter thaueri Miller and Lin 2002
@ref: 4574
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobrevibacter
species: Methanobrevibacter thaueri
full scientific name: Methanobrevibacter thaueri Miller and Lin 2002
strain designation: CW
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 4574 | MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) | yes | https://mediadive.dsmz.de/medium/1523 | Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na-acetate 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water |
| 4574 | METHANOBACTERIUM MEDIUM (DSMZ Medium 119) | yes | https://mediadive.dsmz.de/medium/119 | Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water |
culture temp
- @ref: 4574
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4574
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 4574
- sample type: bovine faeces
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Bovinae (Cow, Cattle) |
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
Sequence information
16S sequences
- @ref: 4574
- description: Methanobrevibacter thaueri strain CW 16S ribosomal RNA gene, complete sequence
- accession: U55236
- length: 1268
- database: ena
- NCBI tax ID: 190975
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanobrevibacter thaueri DSM 11995 | GCA_003111625 | contig | ncbi | 190975 |
| 66792 | Methanobrevibacter thaueri DSM 11995 | 2622736427 | draft | img | 190975 |
| 66792 | Methanobrevibacter thaueri strain DSM 11995 | 190975.3 | wgs | patric | 190975 |
GC content
- @ref: 4574
- GC-content: 37.6
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 4574
culture collection no.: DSM 11995, OCM 817
straininfo link
- @ref: 76436
- straininfo: 49220
literature
- topic: Metabolism
- Pubmed-ID: 30210264
- title: Comparative Genomic Analysis of Members of the Genera Methanosphaera and Methanobrevibacter Reveals Distinct Clades with Specific Potential Metabolic Functions.
- authors: Poehlein A, Schneider D, Soh M, Daniel R, Seedorf H
- journal: Archaea
- DOI: 10.1155/2018/7609847
- year: 2018
- mesh: Acetates/metabolism, Ethanol/metabolism, *Genomics, Metabolic Networks and Pathways/*genetics, Methane/*metabolism, Methanobacteriaceae/*classification/*genetics/metabolism, Methanol/metabolism, Nitrogen/metabolism
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4574 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11995) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11995 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76436 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49220.1 | StrainInfo: A central database for resolving microbial strain identifiers |