Strain identifier
BacDive ID: 6973
Type strain: 
Species: Methanobrevibacter gottschalkii
Strain Designation: HO
Strain history: <- T. L. Miller, New York State Department of Health, Albany, New York, USA; HO <- T. L. Miller {1983}
NCBI tax ID(s): 1122229 (strain), 190974 (species)
version 8.1 (current version)
General
@ref: 4565
BacDive-ID: 6973
DSM-Number: 11977
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic, Gram-negative
description: Methanobrevibacter gottschalkii HO is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from horse faeces.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 190974 | species |
| 1122229 | strain |
strain history
- @ref: 4565
- history: <- T. L. Miller, New York State Department of Health, Albany, New York, USA; HO <- T. L. Miller {1983}
doi: 10.13145/bacdive6973.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanobrevibacter
- species: Methanobrevibacter gottschalkii
- full scientific name: Methanobrevibacter gottschalkii Miller and Lin 2002
@ref: 4565
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobrevibacter
species: Methanobrevibacter gottschalkii
full scientific name: Methanobrevibacter gottschalkii Miller and Lin 2002
strain designation: HO
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.794
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 4565 | MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) | yes | https://mediadive.dsmz.de/medium/1523 | Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na-acetate 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water |
| 4565 | METHANOBACTERIUM MEDIUM (DSMZ Medium 119) | yes | https://mediadive.dsmz.de/medium/119 | Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water |
culture temp
- @ref: 4565
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 4565 | anaerobe | |
| 69480 | anaerobe | 97.794 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.962 |
Isolation, sampling and environmental information
isolation
- @ref: 4565
- sample type: horse faeces
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Equidae (Horse) |
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
Safety information
risk assessment
- @ref: 4565
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4565
- description: Methanobrevibacter gottschalkii strain HO 16S ribosomal RNA gene, complete sequence
- accession: U55238
- length: 1328
- database: ena
- NCBI tax ID: 190974
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanobrevibacter gottschalkii DSM 11977 | GCA_003814835 | scaffold | ncbi | 1122229 |
| 66792 | Methanobrevibacter gottschalkii DSM 11977 | 2786546169 | draft | img | 190974 |
| 66792 | Methanobrevibacter gottschalkii DSM 11977 | 2622736425 | draft | img | 190974 |
| 66792 | Methanobrevibacter gottschalkii DSM 11977 | 1122229.3 | wgs | patric | 1122229 |
GC content
- @ref: 4565
- GC-content: 29.2
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 92.438 | no |
| flagellated | no | 96.748 | no |
| gram-positive | no | 94.12 | no |
| anaerobic | yes | 98.173 | yes |
| aerobic | no | 95.034 | yes |
| halophile | no | 83.831 | no |
| spore-forming | no | 94.645 | no |
| thermophile | no | 97.891 | yes |
| glucose-util | yes | 62.217 | no |
| glucose-ferment | no | 53.696 | no |
External links
@ref: 4565
culture collection no.: DSM 11977, OCM 813
straininfo link
- @ref: 76435
- straininfo: 49212
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4565 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11977) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11977 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 76435 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49212.1 | StrainInfo: A central database for resolving microbial strain identifiers |