Strain identifier

BacDive ID: 6970

Type strain: Yes

Species: Methanobrevibacter curvatus

Strain Designation: RFM-2

Strain history: <- J. R. Leadbetter and J. A. Breznak, Michigan State University, Center for Microbial Ecology, E. Lansing, USA; RFM-2 <- J. R. Leadbetter and J. A. Breznak {1995}

NCBI tax ID(s): 49547 (species)

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General

@ref: 4207

BacDive-ID: 6970

DSM-Number: 11111

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobrevibacter curvatus RFM-2 is an anaerobe, mesophilic archaeon that was isolated from hindgut of termite Reticulitermes flavipes.

NCBI tax id

  • NCBI tax id: 49547
  • Matching level: species

strain history

  • @ref: 4207
  • history: <- J. R. Leadbetter and J. A. Breznak, Michigan State University, Center for Microbial Ecology, E. Lansing, USA; RFM-2 <- J. R. Leadbetter and J. A. Breznak {1995}

doi: 10.13145/bacdive6970.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanobrevibacter
  • species: Methanobrevibacter curvatus
  • full scientific name: Methanobrevibacter curvatus Leadbetter and Breznak 1997

@ref: 4207

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobrevibacter

species: Methanobrevibacter curvatus

full scientific name: Methanobrevibacter curvatus Leadbetter and Breznak 1997

strain designation: RFM-2

type strain: yes

Morphology

colony morphology

  • @ref: 4207
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 4207
  • name: METHANOBREVIBACTER CURVATUS MEDIUM (DSMZ Medium 734)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/734
  • composition: Name: METHANOBREVIBACTER CURVATUS MEDIUM (DSMZ Medium 734) Composition: MOPS 2.1 g/l Na2CO3 2.0 g/l Nutrient broth 2.0 g/l NaCl 1.0 g/l Casamino acids 0.5 g/l KCl 0.5 g/l Yeast extract 0.5 g/l MgCl2 x 6 H2O 0.4 g/l NH4Cl 0.3 g/l KH2PO4 0.2 g/l DL-Dithiothreitol 0.16 g/l Na2SO4 0.15 g/l CaCl2 x 2 H2O 0.1 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l NaOH 0.0005 g/l Sodium resazurin 0.0005 g/l Pyridoxine hydrochloride 0.0003 g/l Thiamine-HCl x 2 H2O 0.0002 g/l Nicotinic acid 0.0002 g/l CoCl2 x 6 H2O 0.00019 g/l Vitamin B12 0.0001 g/l Calcium pantothenate 0.0001 g/l MnCl2 x 4 H2O 0.0001 g/l p-Aminobenzoic acid 8e-05 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l D-(+)-biotin 2e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Rumen fluid Distilled water

culture temp

  • @ref: 4207
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4207
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 4207
  • sample type: hindgut of termite Reticulitermes flavipes
  • host species: Reticulitermes flavipes
  • geographic location: Michigan, Dansville
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_190230.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17907;96_70236;97_91207;98_123937;99_190230&stattab=map
  • Last taxonomy: Methanobrevibacter
  • 16S sequence: U62533
  • Sequence Identity:
  • Total samples: 6
  • aquatic counts: 2
  • animal counts: 4

Safety information

risk assessment

  • @ref: 4207
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4207
  • description: Methanobrevibacter curvatus 16S ribosomal RNA gene, partial sequence
  • accession: U62533
  • length: 1364
  • database: ena
  • NCBI tax ID: 49547

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanobrevibacter curvatus DSM 11111GCA_001639295contigncbi49547
66792Methanobrevibacter curvatus DSM111112684622742draftimg49547
66792Methanobrevibacter curvatus strain DSM 1111149547.3wgspatric49547

GC content

  • @ref: 4207
  • GC-content: 25.7
  • method: sequence analysis

External links

@ref: 4207

culture collection no.: DSM 11111

straininfo link

  • @ref: 76432
  • straininfo: 49035

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8837417Physiological ecology of Methanobrevibacter cuticularis sp. nov. and Methanobrevibacter curvatus sp. nov., isolated from the hindgut of the termite Reticulitermes flavipes.Leadbetter JR, Breznak JAAppl Environ Microbiol10.1128/aem.62.10.3620-3631.19961996Animals, Catalase/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Digestive System/microbiology, Ecology, Insecta/*microbiology, Methane/metabolism, Methanobacteriaceae/classification/drug effects/*isolation & purification/*physiology, Molecular Sequence Data, Oxygen/pharmacology, Phylogeny, RNA, Ribosomal/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic AcidMetabolism
Genetics27340077Draft Genome Sequences of Methanobrevibacter curvatus DSM11111, Methanobrevibacter cuticularis DSM11139, Methanobrevibacter filiformis DSM11501, and Methanobrevibacter oralis DSM7256.Poehlein A, Seedorf HGenome Announc10.1128/genomeA.00617-162016

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4207Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11111)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11111
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76432Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49035.1StrainInfo: A central database for resolving microbial strain identifiers