Strain identifier

BacDive ID: 697

Type strain: No

Species: Bacillus licheniformis

Strain Designation: 1.1B, 1.1.B

Strain history: CIP <- 2003, C. Parini, Milan Univ., Milan, Italy: strain 1.1.B

NCBI tax ID(s): 1409 (species)

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General

@ref: 4624

BacDive-ID: 697

DSM-Number: 12369

keywords: genome sequence, Bacteria, facultative anaerobe, spore-forming, thermophilic, motile

description: Bacillus licheniformis 1.1B is a facultative anaerobe, spore-forming, thermophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 1409
  • Matching level: species

strain history

@refhistory
4624<- C. Parini; 1.1B
367022003, C. Parini, Milano, Italy: strain 1.1.B
120594CIP <- 2003, C. Parini, Milan Univ., Milan, Italy: strain 1.1.B

doi: 10.13145/bacdive697.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus licheniformis
  • full scientific name: Bacillus licheniformis (Weigmann 1898) Chester 1901 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Clostridium licheniforme

@ref: 4624

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus licheniformis

full scientific name: Bacillus sp.

strain designation: 1.1B, 1.1.B

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes94.772
69480100positive
120594yespositiverod-shaped

colony morphology

  • @ref: 120594

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4624NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36702MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120594CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4624positivegrowth42thermophilic
36702positivegrowth37mesophilic
120594positivegrowth22-55
120594nogrowth10psychrophilic

culture pH

  • @ref: 120594
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 120594
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100
120594yes

halophily

  • @ref: 120594
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin+builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
120594citrate-carbon source16947
120594esculin+hydrolysis4853
120594hippurate+hydrolysis606565
120594nitrate+reduction17632
120594nitrite+reduction16301
120594nitrate+respiration17632

metabolite production

  • @ref: 120594
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12059415688acetoin+
12059417234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120594oxidase-
120594beta-galactosidase+3.2.1.23
120594alcohol dehydrogenase+1.1.1.1
120594gelatinase+
120594amylase+
120594DNase+
120594caseinase-3.4.21.50
120594catalase+1.11.1.6
120594tween esterase-
120594gamma-glutamyltransferase+2.3.2.2
120594lecithinase-
120594lipase-
120594lysine decarboxylase-4.1.1.18
120594ornithine decarboxylase-4.1.1.17
120594protease-
120594urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120594-+++-+----++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120594+--+++---++++-+--++-+++++++++-+++-+++-++-+----+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120594+++++--+-++++--+++++++++------++-++++----+-++--++++--++++--++------------+-+---++++---+--++++++-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4624soilSouth-East region of PekingChinaCHNAsia
120594Environment, SoilChinaCHNAsia1995

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 120594
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus paralicheniformis DSM 12369GCA_007831315scaffoldncbi1648923
66792Bacillus paralicheniformis strain DSM 123691648923.82wgspatric1648923

GC content

  • @ref: 4624
  • GC-content: 47
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes89.006no
flagellatedyes85.733no
gram-positiveyes88.83no
anaerobicno98.626no
aerobicyes79.498no
halophileyes65.83no
spore-formingyes97.084no
thermophileno97.185no
glucose-utilyes89.433no
glucose-fermentno88.763no

External links

@ref: 4624

culture collection no.: DSM 12369, CIP 108208

straininfo link

  • @ref: 70372
  • straininfo: 49279

Reference

@idauthorscataloguedoi/urltitle
4624Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12369)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12369
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36702Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5805
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70372Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49279.1StrainInfo: A central database for resolving microbial strain identifiers
120594Curators of the CIPCollection of Institut Pasteur (CIP 108208)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108208