Strain identifier

BacDive ID: 6955

Type strain: Yes

Species: Methanobacterium movens

Strain Designation: TS-2

Strain history: X. Dong and J. Zhu TS-2.

NCBI tax ID(s): 493461 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 18137

BacDive-ID: 6955

DSM-Number: 25945

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobacterium movens TS-2 is an anaerobe, mesophilic archaeon that was isolated from sediments.

NCBI tax id

  • NCBI tax id: 493461
  • Matching level: species

strain history

@refhistory
18137<- T. Itoh, JCM <- X. Dong and J. Zhu; TS-2
67770X. Dong and J. Zhu TS-2.

doi: 10.13145/bacdive6955.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanobacterium
  • species: Methanobacterium movens
  • full scientific name: Methanobacterium movens Zhu et al. 2011

@ref: 18137

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium movens

full scientific name: Methanobacterium movens Zhu et al. 2011

strain designation: TS-2

type strain: yes

Morphology

colony morphology

  • @ref: 18137
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 18137
  • name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/119
  • composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

@refgrowthtypetemperaturerange
18137positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 18137
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
18137sedimentsQinghai, Tuosu lake in the Qaidam basinChinaCHNAsia
67770Sediments of Tuosu lake in the Qaidam basinQinghaiChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Sediment

Safety information

risk assessment

  • @ref: 18137
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18137
  • description: Methanobacterium movens strain TS-2 16S ribosomal RNA gene, partial sequence
  • accession: EU366499
  • length: 1443
  • database: ena
  • NCBI tax ID: 493461

GC content

@refGC-contentmethod
1813739.1thermal denaturation, midpoint method (Tm)
6777039.5high performance liquid chromatography (HPLC)

External links

@ref: 18137

culture collection no.: DSM 25945, AS 1.5093, JCM 15415, CGMCC 1.5093

straininfo link

  • @ref: 76417
  • straininfo: 407785

literature

  • topic: Phylogeny
  • Pubmed-ID: 21278411
  • title: Methanobacterium movens sp. nov. and Methanobacterium flexile sp. nov., isolated from lake sediment.
  • authors: Zhu J, Liu X, Dong X
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.027540-0
  • year: 2011
  • mesh: Chemoautotrophic Growth, China, DNA, Archaeal/genetics, DNA, Ribosomal/genetics, Geologic Sediments/*microbiology, Hydrogen/metabolism, Lakes/*microbiology, Methane/metabolism, Methanobacterium/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
18137Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25945)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25945
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
76417Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID407785.1StrainInfo: A central database for resolving microbial strain identifiers