Strain identifier
BacDive ID: 6954
Type strain:
Species: Methanobacterium flexile
Strain Designation: GH, AS.1.5092
Strain history: X. Dong and J. Zhu strain GH.
NCBI tax ID(s): 490098 (species)
General
@ref: 18131
BacDive-ID: 6954
DSM-Number: 25939
keywords: 16S sequence, Archaea, anaerobe, mesophilic
description: Methanobacterium flexile GH is an anaerobe, mesophilic archaeon that was isolated from sediments.
NCBI tax id
- NCBI tax id: 490098
- Matching level: species
strain history
@ref | history |
---|---|
18131 | <- T. Itoh, JCM <- X. Dong and J. Zhu; GH |
67770 | X. Dong and J. Zhu strain GH. |
doi: 10.13145/bacdive6954.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanobacterium
- species: Methanobacterium flexile
- full scientific name: Methanobacterium flexile Zhu et al. 2011
@ref: 18131
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobacterium
species: Methanobacterium flexile
full scientific name: Methanobacterium flexile Zhu et al. 2011
strain designation: GH, AS.1.5092
type strain: yes
Morphology
colony morphology
- @ref: 18131
- incubation period: 2-3 days
Culture and growth conditions
culture medium
- @ref: 18131
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
18131 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 18131
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
18131 | sediments | Qinghai, Gahai lake in the Qaidam basin | China | CHN | Asia |
67770 | Sediments of Gahai lake in the Qaidam basin | Qinghai | China | CHN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Sediment
Safety information
risk assessment
- @ref: 18131
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 18131
- description: Methanobacterium flexile strain GH 16S ribosomal RNA gene, partial sequence
- accession: EU333914
- length: 1444
- database: ena
- NCBI tax ID: 490098
GC content
@ref | GC-content | method |
---|---|---|
18131 | 36.4 | thermal denaturation, midpoint method (Tm) |
67770 | 37.3 | high performance liquid chromatography (HPLC) |
External links
@ref: 18131
culture collection no.: DSM 25939, AS 1.5092, JCM 15416, CGMCC 1.5092
straininfo link
- @ref: 76416
- straininfo: 407279
literature
- topic: Phylogeny
- Pubmed-ID: 21278411
- title: Methanobacterium movens sp. nov. and Methanobacterium flexile sp. nov., isolated from lake sediment.
- authors: Zhu J, Liu X, Dong X
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.027540-0
- year: 2011
- mesh: Chemoautotrophic Growth, China, DNA, Archaeal/genetics, DNA, Ribosomal/genetics, Geologic Sediments/*microbiology, Hydrogen/metabolism, Lakes/*microbiology, Methane/metabolism, Methanobacterium/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
18131 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25939) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25939 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
76416 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID407279.1 | StrainInfo: A central database for resolving microbial strain identifiers |