General

@ref: 16041

BacDive-ID: 6953

DSM-Number: 22026

keywords: 16S sequence, Archaea, anaerobe, thermophilic

description: Methanobacterium kanagiense 169 is an anaerobe, thermophilic archaeon that was isolated from rice field soil.

NCBI tax id

• NCBI tax id: 483896
• Matching level: species

strain history

doi: 10.13145/bacdive6953.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanobacteria
• order: Methanobacteriales
• family: Methanobacteriaceae
• genus: Methanobacterium
• species: Methanobacterium kanagiense
• full scientific name: Methanobacterium kanagiense Kitamura et al. 2011

@ref: 16041

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium kanagiense

full scientific name: Methanobacterium kanagiense Kitamura et al. 2011

strain designation: 169

type strain: yes

Morphology

colony morphology

• @ref: 16041
• incubation period: 2-3 days

Culture and growth conditions

culture medium

• @ref: 16041
• name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
• growth: yes
• link: https://mediadive.dsmz.de/medium/119
• composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

Physiology and metabolism

oxygen tolerance

• @ref: 16041
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

isolation source categories

Safety information

risk assessment

• @ref: 16041
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 16041
• description: Methanobacterium kanagiense gene for 16S ribosomal RNA, partial sequence
• accession: AB368917
• length: 1465
• database: ena
• NCBI tax ID: 483896

GC content

• @ref: 16041
• GC-content: 39.3
• method: high performance liquid chromatography (HPLC)

External links

@ref: 16041

culture collection no.: DSM 22026, JCM 15797, NBRC 107744

straininfo link

• @ref: 76415
• straininfo: 401523

literature

• topic: Phylogeny
• Pubmed-ID: 20639228
• title: Methanobacterium kanagiense sp. nov., a hydrogenotrophic methanogen, isolated from rice-field soil.
• authors: Kitamura K, Fujita T, Akada S, Tonouchi A
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.026013-0
• year: 2010
• mesh: Anaerobiosis, Autotrophic Processes, Base Composition, Carbon Dioxide, Cell Wall/ultrastructure, Cluster Analysis, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Hydrogen/*metabolism, Hydrogen-Ion Concentration, Japan, Methane/metabolism, Methanobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Oryza/growth & development, Phylogeny, Propionates/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature
• topic2: Metabolism

Reference