General

@ref: 16070

BacDive-ID: 6951

DSM-Number: 21974

keywords: 16S sequence, Archaea, anaerobe, mesophilic, Gram-variable, rod-shaped

description: Methanobacterium ferruginis Mic6c05 is an anaerobe, mesophilic, Gram-variable archaeon that was isolated from iron-corrosive tubercle on an interior pipe transporting natural-gas-containing brine.

NCBI tax id

• NCBI tax id: 710191
• Matching level: species

strain history

• @ref: 16070
• history: <- K. Mori, Natl. Inst. of Technol. and Evaluation (NITE), Japan

doi: 10.13145/bacdive6951.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanobacteria
• order: Methanobacteriales
• family: Methanobacteriaceae
• genus: Methanobacterium
• species: Methanobacterium ferruginis
• full scientific name: Methanobacterium ferruginis Mori and Harayama 2011

@ref: 16070

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium ferruginis

full scientific name: Methanobacterium ferruginis Mori and Harayama 2011

strain designation: Mic6c05

type strain: yes

Morphology

cell morphology

• @ref: 29854
• gram stain: variable
• cell length: 3 µm
• cell width: 0.3 µm
• cell shape: rod-shaped
• motility: no

colony morphology

• @ref: 16070
• incubation period: 3-7 days

Culture and growth conditions

culture medium

• @ref: 16070
• name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141
• composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

halophily

Isolation, sampling and environmental information

isolation

• @ref: 16070
• sample type: iron-corrosive tubercle on an interior pipe transporting natural-gas-containing brine
• geographic location: Chiba prefecture, southern Kanto gas field
• country: Japan
• origin.country: JPN
• continent: Asia

isolation source categories

Safety information

risk assessment

• @ref: 16070
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 16070
• description: Methanobacterium ferruginis gene for 16S ribosomal RNA, partial sequence
• accession: AB542743
• length: 1412
• database: ena
• NCBI tax ID: 710191

GC content

External links

@ref: 16070

culture collection no.: DSM 21974, NBRC 105197

straininfo link

• @ref: 76413
• straininfo: 397101

literature

• topic: Phylogeny
• Pubmed-ID: 20173004
• title: Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., mesophilic methanogens isolated from salty environments.
• authors: Mori K, Harayama S
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.022723-0
• year: 2010
• mesh: Alcohols/metabolism, Archaeal Proteins/genetics, Carbon Dioxide/metabolism, Cluster Analysis, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Hydrogen/metabolism, Hydrogen-Ion Concentration, Methane/metabolism, Methanobacterium/*classification/genetics/*isolation & purification, Molecular Sequence Data, Oxidoreductases/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Sodium Chloride/metabolism, Temperature
• topic2: Metabolism

Reference