Strain identifier

BacDive ID: 6948

Type strain: Yes

Species: Methanobacterium aarhusense

Strain Designation: H2-LR

Strain history: <- A. G. Shlimon, University of Aarhus, Department of Microbiology, Institute of Biological Sciences, Denmark; H2-LR <- A. G. Shlimon {2001}

NCBI tax ID(s): 256826 (species)

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General

@ref: 5763

BacDive-ID: 6948

DSM-Number: 15219

keywords: 16S sequence, Archaea, anaerobe, thermophilic

description: Methanobacterium aarhusense H2-LR is an anaerobe, thermophilic archaeon that was isolated from marine sediment.

NCBI tax id

  • NCBI tax id: 256826
  • Matching level: species

strain history

  • @ref: 5763
  • history: <- A. G. Shlimon, University of Aarhus, Department of Microbiology, Institute of Biological Sciences, Denmark; H2-LR <- A. G. Shlimon {2001}

doi: 10.13145/bacdive6948.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanobacterium
  • species: Methanobacterium aarhusense
  • full scientific name: Methanobacterium aarhusense Shlimon et al. 2004

@ref: 5763

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium aarhusense

full scientific name: Methanobacterium aarhusense Shlimon et al. 2004

strain designation: H2-LR

type strain: yes

Morphology

colony morphology

  • @ref: 5763
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 5763
  • name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/141
  • composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

  • @ref: 5763
  • growth: positive
  • type: growth
  • temperature: 40
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5763
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 5763
  • sample type: marine sediment
  • geographic location: Arhus Bay
  • country: Denmark
  • origin.country: DNK
  • continent: Europe
  • latitude: 56.1556
  • longitude: 10.3233

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_190297.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_69725;97_90544;98_123061;99_190297&stattab=map
  • Last taxonomy: Methanobacterium aarhusense subclade
  • 16S sequence: AY386124
  • Sequence Identity:
  • Total samples: 269
  • soil counts: 18
  • aquatic counts: 227
  • animal counts: 13
  • plant counts: 11

Safety information

risk assessment

  • @ref: 5763
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5763
  • description: Methanobacterium aarhusense 16S ribosomal RNA gene, partial sequence
  • accession: AY386124
  • length: 1333
  • database: ena
  • NCBI tax ID: 256826

GC content

  • @ref: 5763
  • GC-content: 34.9

External links

@ref: 5763

culture collection no.: DSM 15219, ATCC BAA 828, NBRC 105225

straininfo link

  • @ref: 76410
  • straininfo: 132021

literature

  • topic: Phylogeny
  • Pubmed-ID: 15143021
  • title: Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark).
  • authors: Shlimon AG, Friedrich MW, Niemann H, Ramsing NB, Finster K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02994-0
  • year: 2004
  • mesh: Base Composition, Base Sequence, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Denmark, Geologic Sediments/microbiology, Methane/metabolism, Methanobacterium/*classification/genetics/*isolation & purification/metabolism, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
5763Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15219)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15219
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76410Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132021.1StrainInfo: A central database for resolving microbial strain identifiers