Strain identifier
BacDive ID: 6947
Type strain:
Species: Methanobacterium beijingense
Strain Designation: 8-2
Strain history: <- X. Dong, State Key Lab. Microbial Resources, Inst. Microbiol., Chinese Academy of Sciences, Beijing, China <- K. Ma {2002}
NCBI tax ID(s): 242129 (species)
General
@ref: 6232
BacDive-ID: 6947
DSM-Number: 15999
keywords: 16S sequence, Archaea, anaerobe, mesophilic
description: Methanobacterium beijingense 8-2 is an anaerobe, mesophilic archaeon that was isolated from anaerobic digester for the treatment of beer-manufacture wastewater.
NCBI tax id
- NCBI tax id: 242129
- Matching level: species
strain history
- @ref: 6232
- history: <- X. Dong, State Key Lab. Microbial Resources, Inst. Microbiol., Chinese Academy of Sciences, Beijing, China <- K. Ma {2002}
doi: 10.13145/bacdive6947.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanobacterium
- species: Methanobacterium beijingense
- full scientific name: Methanobacterium beijingense Ma et al. 2005
@ref: 6232
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobacterium
species: Methanobacterium beijingense
full scientific name: Methanobacterium beijingense Ma et al. 2005
strain designation: 8-2
type strain: yes
Culture and growth conditions
culture medium
- @ref: 6232
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
culture temp
- @ref: 6232
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 6232
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 6232
- sample type: anaerobic digester for the treatment of beer-manufacture wastewater
- geographic location: Beijing
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Biodegradation | #Anaerobic digestor |
#Engineered | #Industrial | |
#Engineered | #Waste | #Industrial wastewater |
#Condition | #Anoxic (anaerobic) |
taxonmaps
- @ref: 69479
- File name: preview.99_190403.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_69752;97_90575;98_123761;99_190403&stattab=map
- Last taxonomy: Methanobacterium beijingense subclade
- 16S sequence: AY350742
- Sequence Identity:
- Total samples: 692
- soil counts: 102
- aquatic counts: 485
- animal counts: 11
- plant counts: 94
Safety information
risk assessment
- @ref: 6232
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6232
- description: Methanobacterium beijingense strain 8-2 16S ribosomal RNA gene, partial sequence
- accession: AY350742
- length: 1416
- database: ena
- NCBI tax ID: 242129
GC content
- @ref: 6232
- GC-content: 38.9
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 6232
culture collection no.: DSM 15999, CGMCC 1.5011
straininfo link
- @ref: 76409
- straininfo: 135122
literature
- topic: Phylogeny
- Pubmed-ID: 15653895
- title: Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters.
- authors: Ma K, Liu X, Dong X
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63254-0
- year: 2005
- mesh: Anaerobiosis, *Bioreactors, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Methane/*metabolism, Methanobacterium/*classification/genetics/isolation & purification/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
6232 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15999) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15999 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
76409 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID135122.1 | StrainInfo: A central database for resolving microbial strain identifiers |