Strain identifier

BacDive ID: 6947

Type strain: Yes

Species: Methanobacterium beijingense

Strain Designation: 8-2

Strain history: <- X. Dong, State Key Lab. Microbial Resources, Inst. Microbiol., Chinese Academy of Sciences, Beijing, China <- K. Ma {2002}

NCBI tax ID(s): 242129 (species)

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General

@ref: 6232

BacDive-ID: 6947

DSM-Number: 15999

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobacterium beijingense 8-2 is an anaerobe, mesophilic archaeon that was isolated from anaerobic digester for the treatment of beer-manufacture wastewater.

NCBI tax id

  • NCBI tax id: 242129
  • Matching level: species

strain history

  • @ref: 6232
  • history: <- X. Dong, State Key Lab. Microbial Resources, Inst. Microbiol., Chinese Academy of Sciences, Beijing, China <- K. Ma {2002}

doi: 10.13145/bacdive6947.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanobacterium
  • species: Methanobacterium beijingense
  • full scientific name: Methanobacterium beijingense Ma et al. 2005

@ref: 6232

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium beijingense

full scientific name: Methanobacterium beijingense Ma et al. 2005

strain designation: 8-2

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 6232
  • name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/119
  • composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

  • @ref: 6232
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 6232
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 6232
  • sample type: anaerobic digester for the treatment of beer-manufacture wastewater
  • geographic location: Beijing
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Anaerobic digestor
#Engineered#Industrial
#Engineered#Waste#Industrial wastewater
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_190403.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_69752;97_90575;98_123761;99_190403&stattab=map
  • Last taxonomy: Methanobacterium beijingense subclade
  • 16S sequence: AY350742
  • Sequence Identity:
  • Total samples: 692
  • soil counts: 102
  • aquatic counts: 485
  • animal counts: 11
  • plant counts: 94

Safety information

risk assessment

  • @ref: 6232
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6232
  • description: Methanobacterium beijingense strain 8-2 16S ribosomal RNA gene, partial sequence
  • accession: AY350742
  • length: 1416
  • database: ena
  • NCBI tax ID: 242129

GC content

  • @ref: 6232
  • GC-content: 38.9
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 6232

culture collection no.: DSM 15999, CGMCC 1.5011

straininfo link

  • @ref: 76409
  • straininfo: 135122

literature

  • topic: Phylogeny
  • Pubmed-ID: 15653895
  • title: Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters.
  • authors: Ma K, Liu X, Dong X
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63254-0
  • year: 2005
  • mesh: Anaerobiosis, *Bioreactors, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Methane/*metabolism, Methanobacterium/*classification/genetics/isolation & purification/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
6232Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15999)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15999
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76409Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID135122.1StrainInfo: A central database for resolving microbial strain identifiers