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Strain identifier

BacDive ID: 6946

Type strain: Yes

Species: Methanobacterium congolense

Strain Designation: C

Strain history: <- J.-L. Garcia; C

NCBI tax ID(s): 118062 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2982

BacDive-ID: 6946

DSM-Number: 7095

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobacterium congolense C is an anaerobe, mesophilic archaeon that was isolated from methanogenic digestor fed with cassava peel.

NCBI tax id

  • NCBI tax id: 118062
  • Matching level: species

strain history: <- J.-L. Garcia; C

doi: 10.13145/bacdive6946.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/euryarchaeota
  • domain: Archaea
  • phylum: Euryarchaeota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanobacterium
  • species: Methanobacterium congolense
  • full scientific name: Methanobacterium congolense Cuzin et al. 2001

@ref: 2982

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium congolense

full scientific name: Methanobacterium congolense Cuzin et al. 2001

strain designation: C

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 97.263

colony morphology

  • @ref: 2982
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 2982
  • name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/119
  • composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

  • @ref: 2982
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
2982anaerobe
69480anaerobe99.598

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 93.817

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 90.957

Isolation, sampling and environmental information

isolation

  • @ref: 2982
  • sample type: methanogenic digestor fed with cassava peel
  • country: Congo, Republic of the
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Anaerobic digestor
#Host Body-Site#Plant#Fruit (Seed)

Safety information

risk assessment

  • @ref: 2982
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 2982
  • description: Methanobacterium congolense 16S ribosomal RNA gene, partial sequence
  • accession: AF233586
  • length: 1453
  • database: ena
  • NCBI tax ID: 118062

GC content

  • @ref: 2982
  • GC-content: 40

External links

@ref: 2982

culture collection no.: DSM 7095, NBRC 105227

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/160531

literature

  • topic: Phylogeny
  • Pubmed-ID: 11321095
  • title: Methanobacterium congolense sp. nov., from a methanogenic fermentation of cassava peel.
  • authors: Cuzin N, Ouattara AS, Labat M, Garcia JL
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-2-489
  • year: 2001
  • mesh: Bioreactors, DNA, Ribosomal, *Fermentation, Food Handling, Manihot/*microbiology, Methanobacterium/*classification/isolation & purification, Molecular Sequence Data, RNA, Ribosomal, 16S, Terminology as Topic
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2982Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7095)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7095
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)