General

@ref: 2982

BacDive-ID: 6946

DSM-Number: 7095

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobacterium congolense C is an anaerobe, mesophilic archaeon that was isolated from methanogenic digestor fed with cassava peel.

NCBI tax id

• NCBI tax id: 118062
• Matching level: species

strain history

• @ref: 2982
• history: <- J.-L. Garcia; C

doi: 10.13145/bacdive6946.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanobacteria
• order: Methanobacteriales
• family: Methanobacteriaceae
• genus: Methanobacterium
• species: Methanobacterium congolense
• full scientific name: Methanobacterium congolense Cuzin et al. 2001

@ref: 2982

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium congolense

full scientific name: Methanobacterium congolense Cuzin et al. 2001

strain designation: C

type strain: yes

Morphology

colony morphology

• @ref: 2982
• incubation period: 2-3 days

Culture and growth conditions

culture medium

• @ref: 2982
• name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
• growth: yes
• link: https://mediadive.dsmz.de/medium/119
• composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

• @ref: 2982
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 2982
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 2982
• sample type: methanogenic digestor fed with cassava peel
• country: Republic of the Congo
• origin.country: COG
• continent: Africa

isolation source categories

Safety information

risk assessment

• @ref: 2982
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 2982
• description: Methanobacterium congolense 16S ribosomal RNA gene, partial sequence
• accession: AF233586
• length: 1453
• database: ena
• NCBI tax ID: 118062

GC content

• @ref: 2982
• GC-content: 40

External links

@ref: 2982

culture collection no.: DSM 7095, NBRC 105227

straininfo link

• @ref: 76408
• straininfo: 48363

literature

• topic: Phylogeny
• Pubmed-ID: 11321095
• title: Methanobacterium congolense sp. nov., from a methanogenic fermentation of cassava peel.
• authors: Cuzin N, Ouattara AS, Labat M, Garcia JL
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/00207713-51-2-489
• year: 2001
• mesh: Bioreactors, DNA, Ribosomal, *Fermentation, Food Handling, Manihot/*microbiology, Methanobacterium/*classification/isolation & purification, Molecular Sequence Data, RNA, Ribosomal, 16S, Terminology as Topic
• topic2: Genetics

Reference