General

@ref: 2409

BacDive-ID: 6942

DSM-Number: 5982

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobacterium espanolae GP9 is an anaerobe, mesophilic archaeon that was isolated from anoxic primary sludge, bleach kraft mill.

NCBI tax id

• NCBI tax id: 83565
• Matching level: species

strain history

• @ref: 2409
• history: <- G.B. Patel, GP9

doi: 10.13145/bacdive6942.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanobacteria
• order: Methanobacteriales
• family: Methanobacteriaceae
• genus: Methanobacterium
• species: Methanobacterium espanolae
• full scientific name: Methanobacterium espanolae Patel et al. 1990

@ref: 2409

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium espanolae

full scientific name: Methanobacterium espanolae Patel et al. 1990

strain designation: GP9

type strain: yes

Morphology

colony morphology

• @ref: 2409
• incubation period: 2-3 days

Culture and growth conditions

culture medium

• @ref: 2409
• name: METHANOBACTERIUM ESPANOLAE MEDIUM (DSMZ Medium 506)
• growth: yes
• link: https://mediadive.dsmz.de/medium/506
• composition: Name: METHANOBACTERIUM ESPANOLAE MEDIUM (DSMZ Medium 506) Composition: Na-acetate x 3 H2O 2.4728 g/l Na2CO3 0.49456 g/l (NH4)2SO4 0.445104 g/l K2HPO4 0.286845 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l KH2PO4 0.178042 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l ZnSO4 x 7 H2O 0.00178042 g/l CoSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Nicotinic acid 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

• @ref: 2409
• growth: positive
• type: growth
• temperature: 35
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 2409
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 2409
• sample type: anoxic primary sludge, bleach kraft mill
• country: Canada
• origin.country: CAN
• continent: North America

isolation source categories

Safety information

risk assessment

• @ref: 2409
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 2409
• description: Methanobacterium espanolae 16S small subunit ribosomal RNA gene, partial sequence
• accession: AF095260
• length: 1428
• database: ena
• NCBI tax ID: 83565

GC content

• @ref: 2409
• GC-content: 34.0

External links

@ref: 2409

culture collection no.: DSM 5982, NBRC 105228, NRC 5912

straininfo link

• @ref: 76404
• straininfo: 48036

Reference