Strain identifier
BacDive ID: 694
Type strain: 
Species: Bacillus licheniformis
Strain Designation: AMNH 723, BU 171, CCDB b-30, Ford 1, Hankey B13
Strain history: <- IMET <- NCTC <- ATCC <- N.R. Smith
NCBI tax ID(s): 1402 (species)
version 8.1 (current version)
General
@ref: 3401
BacDive-ID: 694
DSM-Number: 8785
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, motile, human pathogen
description: Bacillus licheniformis AMNH 723 is a spore-forming, mesophilic, motile human pathogen that was isolated from milk.
NCBI tax id
- NCBI tax id: 1402
- Matching level: species
strain history
- @ref: 3401
- history: <- IMET <- NCTC <- ATCC <- N.R. Smith
doi: 10.13145/bacdive694.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus licheniformis
- full scientific name: Bacillus licheniformis (Weigmann 1898) Chester 1901 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Clostridium licheniforme
@ref: 3401
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Bacillus
species: Bacillus licheniformis
full scientific name: Bacillus licheniformis (Weigmann 1898) Chester 1901
strain designation: AMNH 723, BU 171, CCDB b-30, Ford 1, Hankey B13
type strain: no
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 95.229 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 3401
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
- @ref: 3401
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
compound production
| @ref | compound |
|---|---|
| 3401 | alpha acetolactate decarboxylase |
| 3401 | amylase |
| 3401 | penicillinase |
| 3401 | 2,3 butanediol |
| 3401 | glycerol |
Isolation, sampling and environmental information
isolation
- @ref: 3401
- sample type: milk
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host Body Product | #Fluids | #Milk |
| #Engineered | #Food production | #Dairy product |
taxonmaps
- @ref: 69479
- File name: preview.99_138.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_17;97_17;98_122;99_138&stattab=map
- Last taxonomy: Bacillus licheniformis
- 16S sequence: AB680251
- Sequence Identity:
- Total samples: 2539
- soil counts: 475
- aquatic counts: 736
- animal counts: 1197
- plant counts: 131
Safety information
risk assessment
- @ref: 3401
- pathogenicity human: yes, in single cases
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Bacillus licheniformis gene for 16S rRNA, partial sequence, strain: NBRC 12195
- accession: AB680251
- length: 1476
- database: ena
- NCBI tax ID: 1402
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Bacillus licheniformis ATCC 9789 | GCA_002504325 | complete | ncbi | 1402 |
| 66792 | Bacillus licheniformis strain ATCC 9789 | 1402.174 | complete | patric | 1402 |
| 66792 | Bacillus licheniformis ATCC 9789 | 2877772704 | complete | img | 1402 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | yes | 88.378 | no |
| gram-positive | yes | 90.006 | no |
| anaerobic | no | 98.211 | no |
| aerobic | yes | 82.052 | no |
| halophile | yes | 82.04 | no |
| spore-forming | yes | 96.896 | no |
| thermophile | no | 96.22 | yes |
| glucose-util | yes | 89.226 | no |
| motile | yes | 91.417 | no |
| glucose-ferment | no | 88.978 | no |
External links
@ref: 3401
culture collection no.: DSM 8785, ATCC 102, ATCC 4527, ATCC 8243, ATCC 9789, ATCC 9800, CCEB 631, CCM 2205, CN 1060, DSM 46308, HNCMB 101012, IFO 12195, IFO 12196, IMET 11025, NBRC 12195, NBRC 12196, NCDO 735, NCDO 835, NCIMB 6346, NCIMB 8059, NCTC 2586, NCTC 6346, OUT 8367, OUT 8368, Smith 243, Smith 978, NRS 243
straininfo link
- @ref: 70369
- straininfo: 1132
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Pathogenicity | 2468758 | Functional half-life of the alpha-amylase mRNA of Bacillus licheniformis. | Tonkova A, Pazlarova J, Emanuilova E, Stoeva N | J Basic Microbiol | 10.1002/jobm.3620290114 | 1989 | Bacillus/drug effects/enzymology/*genetics/growth & development, Chloramphenicol/pharmacology, Dactinomycin/pharmacology, Gene Expression Regulation, Half-Life, Protein Biosynthesis, RNA, Bacterial/drug effects/*metabolism, RNA, Messenger/drug effects/*metabolism, Transcription, Genetic, alpha-Amylases/biosynthesis/*genetics | Metabolism |
| Cultivation | 3086493 | Regeneration of protoplasts of Bacillus subtilis 168 and closely related strains. | Bourne N, Dancer BN | J Gen Microbiol | 10.1099/00221287-132-2-251 | 1986 | Bacillus subtilis/classification/*physiology, Culture Media, Muramidase, Protoplasts/*physiology, *Regeneration, Transformation, Bacterial | Phylogeny |
| Metabolism | 4988269 | Characterization of inducible bacteriophages in Bacillus licheniformis. | Huang W, Marmur J | J Virol | 10.1128/JVI.5.2.237-246.1970 | 1970 | Bacillus/*metabolism, Bacillus subtilis/metabolism, *Bacteriophages/isolation & purification/metabolism/pathogenicity, Carbon Isotopes, Centrifugation, Centrifugation, Density Gradient, Cesium, Chlorides, DNA, Viral/biosynthesis, Genetics, Microbial, Glycerol, Hybridization, Genetic, Microscopy, Electron, Mitomycins, Molecular Weight, Mutation, Sucrose, Thymidine/metabolism, Thymine/metabolism, Tritium | Enzymology |
| Metabolism | 5003174 | Role of autolysins in the killing of bacteria by some bactericidal antibiotics. | Rogers HJ, Forsberg CW | J Bacteriol | 10.1128/jb.108.3.1235-1243.1971 | 1971 | Autolysis, Bacillus/*drug effects/growth & development/metabolism, Bacillus subtilis/*drug effects/growth & development/metabolism, Bacterial Proteins/biosynthesis, *Bacteriolysis, Carbon Isotopes, Cell Wall/metabolism, Chloramphenicol/pharmacology, Culture Media, Cycloserine/*pharmacology, Genetics, Microbial, Glucose/metabolism, Methicillin/pharmacology, Mucoproteins/biosynthesis, *Mutation, Penicillin Resistance, Spectrophotometry, Vancomycin/*pharmacology | Pathogenicity |
| Enzymology | 5030622 | N-acetylmuramyl-L-alanine amidase of Bacillus licheniformis and its L-form. | Forsberg CW, Ward JB | J Bacteriol | 10.1128/jb.110.3.878-888.1972 | 1972 | Alanine, Amidohydrolases/*metabolism, Amino Sugars, Aspartic Acid/metabolism, Bacillus/*enzymology/growth & development, Binding Sites, Carbon Isotopes, Carboxypeptidases/metabolism, Cell Fractionation, Cell Membrane/enzymology, Cell Wall/enzymology, Centrifugation, Density Gradient, Chromatography, Thin Layer, Detergents, L Forms/*enzymology/growth & development, Muramidase/metabolism, Protoplasts/enzymology, Spectrophotometry, Succinate Dehydrogenase/metabolism | Metabolism |
| Metabolism | 7076624 | Major sites of metal binding in Bacillus licheniformis walls. | Beveridge TJ, Forsberg CW, Doyle RJ | J Bacteriol | 10.1128/jb.150.3.1438-1448.1982 | 1982 | Bacillus/metabolism/*ultrastructure, Binding Sites, Cell Wall/metabolism/ultrastructure, Metals/*metabolism, Peptidoglycan/metabolism, Teichoic Acids/metabolism, Uronic Acids/metabolism | |
| Genetics | 10616719 | Phage abortive infection of Bacillus licheniformis ATCC 9800; identification of the abiBL11 gene and localisation and sequencing of its promoter region. | Tran LS, Szabo L, Ponyi T, Orosz L, Sik T, Holczinger A | Appl Microbiol Biotechnol | 10.1007/s002530051602 | 1999 | Amino Acid Sequence, Bacillus/*genetics/*virology, Bacterial Proteins/genetics, Bacteriophages/isolation & purification/*pathogenicity, Base Sequence, Cloning, Molecular, DNA Transposable Elements/genetics, DNA, Viral/analysis, Genes, Bacterial/genetics, Immunity, Innate/genetics, Molecular Sequence Data, Mutagenesis, Insertional, Nucleic Acid Hybridization, Potassium Channels/genetics, Promoter Regions, Genetic/genetics, Sequence Homology, Nucleic Acid | Enzymology |
| Metabolism | 12699930 | Effects of temperature, inoculum size and starch hydrolyzate concentration on butanediol production by Bacillus licheniformis. | Perego P, Converti A, Del Borghi M | Bioresour Technol | 10.1016/s0960-8524(03)00063-4 | 2003 | Bacillus/*physiology, Bioreactors, Butylene Glycols/*chemistry, Carbon/metabolism, Fermentation, Starch/metabolism, Temperature | Biotechnology |
| Enzymology | 20133129 | Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste. | Songsiriritthigul C, Lapboonrueng S, Pechsrichuang P, Pesatcha P, Yamabhai M | Bioresour Technol | 10.1016/j.biortech.2010.01.036 | 2010 | Bacillus/*enzymology, Base Sequence, Chitinases/genetics/*metabolism, Chromatography, Thin Layer, DNA Primers, Electrophoresis, Polyacrylamide Gel, Escherichia coli/genetics, Hydrogen-Ion Concentration, Hydrolysis, Kinetics, Polymerase Chain Reaction, Recombinant Proteins/genetics/metabolism, Temperature | Metabolism |
| Transcriptome | 22555872 | Design of multiplex PCR for simultaneous detection of rope-forming Bacillus strains in Iranian bread dough. | Sadeghi A, Mortazavi SA, Bahrami AR, Sadeghi B | J Sci Food Agric | 10.1002/jsfa.5681 | 2012 | Bacillus/*genetics, Bacterial Proteins/genetics, Base Sequence, Bread/*microbiology, *DNA Primers, DNA, Bacterial/*analysis, *Genes, Bacterial, Iran, Magnesium Chloride, Membrane Transport Proteins/genetics, Multiplex Polymerase Chain Reaction/*methods, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, Sequence Homology, Temperature | Phylogeny |
| Metabolism | 23722266 | Enhanced 2,3-butanediol production in fed-batch cultures of free and immobilized Bacillus licheniformis DSM 8785. | Jurchescu IM, Hamann J, Zhou X, Ortmann T, Kuenz A, Prusse U, Lang S | Appl Microbiol Biotechnol | 10.1007/s00253-013-4981-z | 2013 | Bacillus/*metabolism, Butylene Glycols/*metabolism, Culture Media, *Fermentation | Cultivation |
| Metabolism | 26445877 | Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic Bacillus licheniformis NCIMB 8059. | Bialkowska AM, Gromek E, Krysiak J, Sikora B, Kalinowska H, Jedrzejczak-Krzepkowska M, Kubik C, Lang S, Schutt F, Turkiewicz M | J Ind Microbiol Biotechnol | 10.1007/s10295-015-1697-3 | 2015 | Aspergillus niger/*enzymology, Bacillus/*metabolism, Batch Cell Culture Techniques, Bioreactors/microbiology, Butylene Glycols/*metabolism, Fermentation, Glucose/metabolism, Hydrolysis, Malus/*chemistry/*metabolism | Biotechnology |
| Biotechnology | 29796086 | Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789. | Rebecchi S, Pinelli D, Zanaroli G, Fava F, Frascari D | Biotechnol Biofuels | 10.1186/s13068-018-1138-4 | 2018 | ||
| Enzymology | 30237128 | Biochemical characterization of chitinase A from Bacillus licheniformis DSM8785 expressed in Pichia pastoris KM71H. | Menghiu G, Ostafe V, Prodanovic R, Fischer R, Ostafe R | Protein Expr Purif | 10.1016/j.pep.2018.09.007 | 2018 | *Bacillus licheniformis/enzymology/genetics, *Bacterial Proteins/biosynthesis/chemistry/genetics/isolation & purification, *Chitinases/biosynthesis/chemistry/genetics/isolation & purification, Enzyme Stability, Hot Temperature, Hydrogen-Ion Concentration, Pichia/enzymology/genetics, Recombinant Proteins/biosynthesis/chemistry/genetics/isolation & purification | Phylogeny |
| Metabolism | 31053124 | Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production. | Heyman B, Lamm R, Tulke H, Regestein L, Buchs J | Microb Cell Fact | 10.1186/s12934-019-1126-9 | 2019 | Bacillus licheniformis/*growth & development/*metabolism, Batch Cell Culture Techniques/*methods, Bioreactors, Butylene Glycols/*metabolism, Fermentation, Glucose/metabolism, Oxygen/*metabolism | Biotechnology |
| Biotechnology | 32874177 | Online monitoring of the respiratory quotient reveals metabolic phases during microaerobic 2,3-butanediol production with Bacillus licheniformis. | Heyman B, Tulke H, Putri SP, Fukusaki E, Buchs J | Eng Life Sci | 10.1002/elsc.201900121 | 2019 | ||
| Metabolism | 33809788 | A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A. | Menghiu G, Ostafe V, Prodanovic R, Fischer R, Ostafe R | Int J Mol Sci | 10.3390/ijms22063041 | 2021 | Catalytic Domain, Cell Survival, Chitinases/*genetics, Cyclodextrins, *Directed Molecular Evolution, *Flow Cytometry, Fluorescent Dyes/metabolism, Gene Library, *High-Throughput Screening Assays, Models, Molecular, Mutation/genetics, Structural Homology, Protein, Substrate Specificity, Trisaccharides, Umbelliferones |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 3401 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8785) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-8785 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 70369 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID1132.1 | StrainInfo: A central database for resolving microbial strain identifiers |