General

@ref: 1448

BacDive-ID: 6937

DSM-Number: 3664

keywords: 16S sequence, Archaea, anaerobe, thermophilic

description: Methanobacterium sp. DSM 3664 is an anaerobe, thermophilic archaeon that was isolated from mesophilic biogas plant.

NCBI tax id

• NCBI tax id: 2164
• Matching level: species

strain history

• @ref: 1448
• history: <- D.R. Boone, CB12

doi: 10.13145/bacdive6937.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanobacteria
• order: Methanobacteriales
• family: Methanobacteriaceae
• genus: Methanobacterium
• species: Methanobacterium sp.
• full scientific name: Methanobacterium Kluyver and van Niel 1936 (Approved Lists 1980)

@ref: 1448

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium sp.

full scientific name: Methanobacterium sp.

type strain: no

Culture and growth conditions

culture medium

• @ref: 1448
• name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
• growth: yes
• link: https://mediadive.dsmz.de/medium/119
• composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

• @ref: 1448
• growth: positive
• type: growth
• temperature: 55-60
• range: thermophilic

Physiology and metabolism

oxygen tolerance

• @ref: 1448
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 1448
• sample type: mesophilic biogas plant
• geographic location: Chengdu
• country: China
• origin.country: CHN
• continent: Asia

isolation source categories

Sequence information

16S sequences

• @ref: 20218
• description: Enterococcus faecalis clone OCM036 16S ribosomal RNA gene, partial sequence
• accession: GU417224
• length: 456
• database: ena
• NCBI tax ID: 1351

External links

@ref: 1448

culture collection no.: DSM 3664, ATCC 43574, OCM 36, CB 12

straininfo link

• @ref: 76400
• straininfo: 47741

literature

• topic: Metabolism
• Pubmed-ID: 8368842
• title: Effects of hydrogen and formate on the degradation of propionate and butyrate in thermophilic granules from an upflow anaerobic sludge blanket reactor.
• authors: Schmidt JE, Ahring BK
• journal: Appl Environ Microbiol
• DOI: 10.1128/aem.59.8.2546-2551.1993
• year: 1993
• mesh: Anaerobiosis, Bacillaceae/metabolism, Biodegradation, Environmental/drug effects, Butyrates/*metabolism, Butyric Acid, Formates/pharmacology, Hydrogen/pharmacology, Methanobacterium/metabolism, Propionates/*metabolism, *Waste Disposal, Fluid
• topic2: Pathogenicity

Reference