Strain identifier

BacDive ID: 6927

Type strain: No

Species: Methanobacterium formicicum

Strain Designation: MS1

Strain history: <- G.D. Vogels, MS1

NCBI tax ID(s): 2162 (species)

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General

@ref: 1426

BacDive-ID: 6927

DSM-Number: 3636

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobacterium formicicum MS1 is an anaerobe, mesophilic archaeon that was isolated from from ciliate Metopus striatus.

NCBI tax id

  • NCBI tax id: 2162
  • Matching level: species

strain history

  • @ref: 1426
  • history: <- G.D. Vogels, MS1

doi: 10.13145/bacdive6927.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanobacterium
  • species: Methanobacterium formicicum
  • full scientific name: Methanobacterium formicicum Schnellen 1947 (Approved Lists 1980)

@ref: 1426

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium formicicum

full scientific name: Methanobacterium formicicum Schnellen 1947 emend. Judicial Commission 1992

strain designation: MS1

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1426MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523)yeshttps://mediadive.dsmz.de/medium/1523Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na-acetate 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water
1426METHANOBACTERIUM MEDIUM (DSMZ Medium 119)yeshttps://mediadive.dsmz.de/medium/119Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

  • @ref: 1426
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1426
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1426
  • sample type: from ciliate Metopus striatus
  • host species: Metopus striatus
  • country: Netherlands
  • origin.country: NLD
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Protozoa

Safety information

risk assessment

  • @ref: 1426
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: M.formicicum (DSM 3636) DNA for 16S ribosomal RNA (partial)
  • accession: Z29436
  • length: 965
  • database: ena
  • NCBI tax ID: 2162

GC content

  • @ref: 1426
  • GC-content: 38.0

External links

@ref: 1426

culture collection no.: DSM 3636

straininfo link

  • @ref: 76391
  • straininfo: 47735

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1426Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3636)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3636
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
76391Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47735.1StrainInfo: A central database for resolving microbial strain identifiers