Strain identifier
BacDive ID: 6901
Type strain: 
Species: Methanobacterium alcaliphilum
Strain Designation: WeN2
Strain history: <- D. R. Boone, University of California, School of Public Health, Los Angeles, USA; WeN2
NCBI tax ID(s): 392018 (species)
General
@ref: 1316
BacDive-ID: 6901
DSM-Number: 3458
keywords: 16S sequence, Archaea, anaerobe, mesophilic
description: Methanobacterium alcaliphilum WeN2 is an anaerobe, mesophilic archaeon that was isolated from high-pH lake sediment.
NCBI tax id
- NCBI tax id: 392018
- Matching level: species
strain history
- @ref: 1316
- history: <- D. R. Boone, University of California, School of Public Health, Los Angeles, USA; WeN2
doi: 10.13145/bacdive6901.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanobacterium
- species: Methanobacterium alcaliphilum
- full scientific name: Methanobacterium alcaliphilum Worakit et al. 1986
@ref: 1316
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobacterium
species: Methanobacterium alcaliphilum
full scientific name: Methanobacterium alcaliphilum Worakit et al. 1986
strain designation: WeN2
type strain: no
Culture and growth conditions
culture medium
- @ref: 1316
- name: METHANOBACTERIUM ALCALIPHILUM MEDIUM (DSMZ Medium 342)
- growth: yes
- link: https://mediadive.dsmz.de/medium/342
- composition: Name: METHANOBACTERIUM ALCALIPHILUM MEDIUM (DSMZ Medium 342) Composition: NaHCO3 9.88141 g/l Trypticase peptone 1.97628 g/l Yeast extract 1.97628 g/l NH4Cl 0.988145 g/l L-Cysteine HCl x H2O 0.494071 g/l Na2CO3 0.494071 g/l K2HPO4 0.395257 g/l MgCl2 x 6 H2O 0.0988145 g/l MgSO4 x 7 H2O 0.0296443 g/l Nitrilotriacetic acid 0.0148221 g/l NaCl 0.00988142 g/l MnSO4 x H2O 0.00494071 g/l ZnSO4 x 7 H2O 0.00177866 g/l CoSO4 x 7 H2O 0.00177866 g/l CaCl2 x 2 H2O 0.000988142 g/l FeSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l NiCl2 x 6 H2O 0.000296443 g/l AlK(SO4)2 x 12 H2O 0.000197628 g/l Na2MoO4 x 2 H2O 9.88142e-05 g/l H3BO3 9.88142e-05 g/l CuSO4 x 5 H2O 9.88142e-05 g/l Na2WO4 x 2 H2O 3.95257e-06 g/l Na2SeO3 x 5 H2O 2.96443e-06 g/l Tris-HCl buffer Distilled water
culture temp
- @ref: 1316
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1316
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 1316
- sample type: high-pH lake sediment
- geographic location: Wadi el Natrun
- country: Egypt
- origin.country: EGY
- continent: Africa
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Aquatic | #Sediment |
| #Condition | #Alkaline |
Safety information
risk assessment
- @ref: 1316
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Corynebacterium matruchotii clone OCM009 16S ribosomal RNA gene, partial sequence
- accession: GU420743
- length: 435
- database: ena
- NCBI tax ID: 43768
External links
@ref: 1316
culture collection no.: DSM 3458, ATCC 43377, OCM 9
straininfo link
- @ref: 76365
- straininfo: 41806
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1316 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3458) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3458 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 76365 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41806.1 | StrainInfo: A central database for resolving microbial strain identifiers |