Archive BacDiveID:10.13145/bacdive6898.20230509.8.1

Strain identifier

BacDive ID: 6898

Type strain:

Species: Methanothermobacter marburgensis

Strain Designation: Marburg

Strain history: NBRC 100331 <-- DSM 2133 <-- R. K. Thauer strain Marburg.

NCBI tax ID(s): 145263 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 972

BacDive-ID: 6898

DSM-Number: 2133

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic

description: Methanothermobacter marburgensis Marburg is an anaerobe, thermophilic archaeon that was isolated from anaerobic sewage digester.

NCBI tax id

NCBI tax id: 145263
Matching level: species

strain history

@ref	history
972	<- R. K. Thauer, Univ. Marburg; Marburg <- G. Fuchs
67770	NBRC 100331 <-- DSM 2133 <-- R. K. Thauer strain Marburg.

doi: 10.13145/bacdive6898.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/methanobacteriota
domain: Archaea
phylum: Methanobacteriota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanothermobacter
species: Methanothermobacter marburgensis
full scientific name: Methanothermobacter marburgensis Wasserfallen et al. 2000

@ref: 972

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanothermobacter

species: Methanothermobacter marburgensis

full scientific name: Methanothermobacter marburgensis Wasserfallen et al. 2000

strain designation: Marburg

type strain: yes

Culture and growth conditions

culture medium

@ref: 972
name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
growth: yes
link: https://mediadive.dsmz.de/medium/119
composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

@ref	growth	type	temperature	range
972	positive	growth	65	thermophilic
67770	positive	growth	65	thermophilic

Physiology and metabolism

oxygen tolerance

@ref: 972
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
972	anaerobic sewage digester	Marburg	Germany	DEU	Europe
67770	Anaerobic sewage digester	Marburg	Germany	DEU	Europe

isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)

taxonmaps

@ref: 69479
File name: preview.99_187127.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_68809;97_89451;98_121724;99_187127&stattab=map
Last taxonomy: Methanothermobacter marburgensis
16S sequence: X15364
Sequence Identity:
Total samples: 257
soil counts: 16
aquatic counts: 185
animal counts: 22
plant counts: 34

Safety information

risk assessment

@ref: 972
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Methanobacterium thermoautotrophicum str. Marburg 16S small subunit ribosomal RNA gene, partial sequence	AF095262	1432	ena	79929
972	Methanothermobacter marburgensis rRNA operon, type strain Marburg	X15364	8709	ena	145263

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Methanothermobacter marburgensis str. Marburg	GCA_000145295	complete	ncbi	79929
66792	Methanothermobacter marburgensis Marburg DSM 2133	648028041	complete	img	79929
66792	Methanothermobacter marburgensis str. Marburg	79929.8	complete	patric	79929
66792	Methanothermobacter marburgensis str. Marburg	79929.14	plasmid	patric	79929
66792	Methanothermobacter marburgensis Marburg	GCA_027555065	complete	ncbi	145263

GC content

@ref: 972
GC-content: 47.6

Genome-based predictions

predictions

trait	prediction	confidence	training_data
motile	no	84.393	no
flagellated	no	96.472	no
gram-positive	no	96.749	no
anaerobic	yes	98.414	yes
aerobic	no	95.242	yes
halophile	no	84.038	no
spore-forming	no	96.653	no
thermophile	yes	87.519	no
glucose-util	yes	74.721	no
glucose-ferment	no	62.122	no

External links

@ref: 972

culture collection no.: DSM 2133, NBRC 100331, OCM 82, JCM 14651

straininfo link

@ref: 76362
straininfo: 47548

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	10826786	Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov.	Wasserfallen A, Nolling J, Pfister P, Reeve J, Conway de Macario E	Int J Syst Evol Microbiol	10.1099/00207713-50-1-43	2000	Antigens, Archaeal/classification, Bacteriophage Typing, Base Sequence, DNA, Archaeal/genetics, DNA, Ribosomal/genetics, Methanobacteriaceae/classification/genetics/immunology/physiology, Methanobacterium/classification/genetics/immunology/physiology, Molecular Sequence Data, Phenotype, Phylogeny, Plasmids, RNA, Ribosomal, 16S/genetics, Sequence Alignment, Sequence Analysis, DNA, Temperature	Genetics
Enzymology	11081790	Regulation of the synthesis of H2-forming methylenetetrahydromethanopterin dehydrogenase (Hmd) and of HmdII and HmdIII in Methanothermobacter marburgensis.	Afting C, Kremmer E, Brucker C, Hochheimer A, Thauer RK	Arch Microbiol	10.1007/s002030000197	2000	Archaeal Proteins/genetics/metabolism, Blotting, Northern, Blotting, Western, Cloning, Molecular, Culture Media, Gene Expression Regulation, Archaeal, Hydrogen/metabolism, Isoenzymes, Methanobacteriaceae/enzymology/genetics/growth & development, Molecular Sequence Data, Nickel/metabolism, Oxidoreductases Acting on CH-NH Group Donors/biosynthesis/genetics, Pterins/metabolism, Sequence Analysis, DNA, Transcription, Genetic	Metabolism
Genetics	11319928	Comparative sequence analysis of plasmids pME2001 and pME2200 of methanothermobacter marburgensis strains Marburg and ZH3.	Luo Y, Leisinger T, Wasserfallen A	Plasmid	10.1006/plas.2000.1493	2001	Adenosine Triphosphatases/biosynthesis, Amino Acid Sequence, Bacterial Proteins/antagonists & inhibitors/genetics, Base Sequence, DNA Helicases/biosynthesis, DNA Replication, DNA, Archaeal/analysis/biosynthesis/chemistry, Escherichia coli, Escherichia coli Proteins, Methanobacteriaceae/genetics, Molecular Sequence Data, Nucleic Acid Conformation, Plasmids/chemistry/genetics, RNA, Antisense/biosynthesis, Sequence Analysis, DNA, Sequence Homology, Amino Acid	Transcriptome
Genetics	11544247	The genome of archaeal prophage PsiM100 encodes the lytic enzyme responsible for autolysis of Methanothermobacter wolfeii.	Luo Y, Pfister P, Leisinger T, Wasserfallen A	J Bacteriol	10.1128/JB.183.19.5788-5792.2001	2001	Bacteriophages/enzymology/genetics, Base Sequence, Endopeptidases/chemistry/genetics/metabolism, Genome, Viral, Lysogeny, Methanobacteriaceae/physiology/*virology, Molecular Sequence Data	Enzymology
	31896626	Complete Genomic Sequence of the Thermophilic and Hydrogenotrophic Methanogen Methanothermobacter sp. Strain KEPCO-1.	Jeon BS, Kim H, Go YW, Kim YG, Joo JS, Choi O, Sang BI	Microbiol Resour Announc	10.1128/MRA.01034-19	2020
Phylogeny	32504807	Comparative genomic analysis reveals starvation survival systems in Methanothermobacter thermautotrophicus DeltaH.	Prathiviraj R, Chellapandi P	Anaerobe	10.1016/j.anaerobe.2020.102216	2020	Comparative Genomic Hybridization, DNA, Archaeal/genetics, Genome, Archaeal, Metabolic Networks and Pathways/genetics, Methanobacteriaceae/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, Stress, Physiological/*genetics	Genetics

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
972	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2133)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-2133
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
76362	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID47548.1	StrainInfo: A central database for resolving microbial strain identifiers