General

@ref: 3085

BacDive-ID: 6897

DSM-Number: 7268

keywords: 16S sequence, Archaea, anaerobe, thermophilic

description: Methanothermobacter thermoflexus IDZ is an anaerobe, thermophilic archaeon that was isolated from sludge, metacrilic wastes digester.

NCBI tax id

• NCBI tax id: 49340
• Matching level: species

strain history

• @ref: 3085
• history: <- S. V. Kotelnikova and A. Ya. Obraztsova, Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Science, Pushchino, Russia; IDZ

doi: 10.13145/bacdive6897.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanobacteria
• order: Methanobacteriales
• family: Methanobacteriaceae
• genus: Methanothermobacter
• species: Methanothermobacter thermoflexus
• full scientific name: Methanothermobacter thermoflexus (Kotelnikova et al. 1994) Boone 2002

• synonyms

  @ref	synonym
  20215	Methanothermobacter thermoflexus
  20215	Methanobacterium thermoflexum

@ref: 3085

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanothermobacter

species: Methanothermobacter thermoflexus

full scientific name: Methanothermobacter thermoflexus (Kotelnikova et al. 1994) Boone 2002

strain designation: IDZ

type strain: yes

Culture and growth conditions

culture medium

• @ref: 3085
• name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141
• composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

• @ref: 3085
• growth: positive
• type: growth
• temperature: 55
• range: thermophilic

Physiology and metabolism

oxygen tolerance

• @ref: 3085
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 3085
• sample type: sludge, metacrilic wastes digester
• country: Russia
• origin.country: RUS

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_188781.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_68809;97_89451;98_122923;99_188781&stattab=map
• Last taxonomy: Methanothermobacter thermoflexus
• 16S sequence: X99047
• Sequence Identity:
• Total samples: 15
• aquatic counts: 14
• animal counts: 1

Safety information

risk assessment

• @ref: 3085
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 3085
• description: M.thermoflexum 16S rRNA gene
• accession: X99047
• length: 1454
• database: ena
• NCBI tax ID: 49340

GC content

• @ref: 3085
• GC-content: 55.1

External links

@ref: 3085

culture collection no.: DSM 7268, ATCC 51444, VKM B-1963

straininfo link

• @ref: 76361
• straininfo: 46907

Reference